NCBiotech
Associate Scientist, Process Development
NCBiotech, Durham, North Carolina, United States, 27703
ABOUT GREENLIGHT
GreenLight Biosciences is using RNA to create a world where plants, people, and the planet can thrive together. The company is developing highly effective agricultural solutions for farmers and beekeepers that are targeted to specific pests and degrade quickly in the environment. Our pipeline includes products to protect honeybees and a range of fruits and vegetables. The GreenLight platform allows us to research, design, and manufacture across multiple product categories including insecticides, fungicides, and herbicides.
For more information, visit www.greenlightbio.com.
Responsibilities
Support early‑stage product development by performing laboratory scale fermentation, RNA production and downstream processing.
Run 10 L fermentation experiment to produce plasmid DNA using strains developed by the strain engineering team; collect samples and assess process metrics such as plasmid DNA titer, yield and quality.
Perform downstream unit operations to recover plasmid DNA; run 10 L RNA synthesis experiment using the plasmid DNA and assess process metrics such as RNA titer, yield and purity.
Troubleshoot and identify opportunities for improvements in RNA production process by doing DoE‑based optimization experiments; design, plan, execute the experiments, collect samples, and run analytical methods.
Analyze data, fit curves, build models, use statistical data analysis tools to validate model predictions, test reproducibility and robustness of the optimized RNA synthesis process conditions.
Lead development of next‑generation processes to improve RNA production metrics; identify limiting parameters, formulate strategies, develop new processes or find better raw materials.
Create tasks, delegate and supervise team members toward completion of project goals.
Develop, author and implement Standard Operating Procedures (SOPs) for process development activities; write protocols and development reports; document in the Quality Management System; train team members on new protocols.
Scale‑up and tech transfer bench‑scale processes to pilot production scale; demonstrate scale‑up by evaluating the new process in 10 L scale; train production team members.
Perform and report on quantitative measurement procedures for analytes (DNA, RNA, proteins, ions, etc.) in a variety of matrices for analytical testing support.
Run RNA analysis methods such as RNA concentration and purity; run DNA analysis methods such as restriction digestion and agarose gel electrophoresis; run HPLC for small molecules, DNA, RNA.
Make data‑driven decisions, identify root causes, and address problems and opportunities.
Test assays on different lots of DNA and enzyme samples to evaluate batch‑to‑batch variability; identify causes for variability using the assay data.
Collaborate with operations and quality management team members; communicate effectively to accomplish project goals and milestones.
Work with cross‑functional teams such as Strain Engineering, Manufacturing, QA/QC; plan data review meetings, present results, suggest directions and next steps.
Requirements
B.S. in Chemical Engineering, Biochemistry or related field.
At least 24 months of experience (or equivalent role) in strain engineering, aseptic techniques, bacterial culture, extraction and characterization of DNA, UV/VIS absorbance, gel electrophoresis, sequencing data analysis.
12 months of experience as a research associate or similar role handling lab‑scale bioreactors for running fermentation and downstream processing equipment such as cell harvest by centrifugation, TFF, DNA column chromatography purification.
12 months of experience as a research associate or similar role handling analytical equipment such as HPLC to quantify DNA, RNA, organic acids.
12 months of experience working with electronic lab notebook, laboratory information management system and statistical software such as GMP.
Equal Employment Opportunity Statement Greenlight Biosciences Inc. provides equal employment opportunities to all employees and applicants for employment and prohibits discrimination and harassment of any type without regard to race, color, religion, age, sex, national origin, disability status, genetics, protected veteran status, sexual orientation, gender identity or expression, or any other characteristic protected by federal, state or local laws. This policy applies to all terms and conditions of employment, including recruiting, hiring, placement, promotion, termination, layoff, recall, transfer, leaves of absence, compensation, and training.
#J-18808-Ljbffr
For more information, visit www.greenlightbio.com.
Responsibilities
Support early‑stage product development by performing laboratory scale fermentation, RNA production and downstream processing.
Run 10 L fermentation experiment to produce plasmid DNA using strains developed by the strain engineering team; collect samples and assess process metrics such as plasmid DNA titer, yield and quality.
Perform downstream unit operations to recover plasmid DNA; run 10 L RNA synthesis experiment using the plasmid DNA and assess process metrics such as RNA titer, yield and purity.
Troubleshoot and identify opportunities for improvements in RNA production process by doing DoE‑based optimization experiments; design, plan, execute the experiments, collect samples, and run analytical methods.
Analyze data, fit curves, build models, use statistical data analysis tools to validate model predictions, test reproducibility and robustness of the optimized RNA synthesis process conditions.
Lead development of next‑generation processes to improve RNA production metrics; identify limiting parameters, formulate strategies, develop new processes or find better raw materials.
Create tasks, delegate and supervise team members toward completion of project goals.
Develop, author and implement Standard Operating Procedures (SOPs) for process development activities; write protocols and development reports; document in the Quality Management System; train team members on new protocols.
Scale‑up and tech transfer bench‑scale processes to pilot production scale; demonstrate scale‑up by evaluating the new process in 10 L scale; train production team members.
Perform and report on quantitative measurement procedures for analytes (DNA, RNA, proteins, ions, etc.) in a variety of matrices for analytical testing support.
Run RNA analysis methods such as RNA concentration and purity; run DNA analysis methods such as restriction digestion and agarose gel electrophoresis; run HPLC for small molecules, DNA, RNA.
Make data‑driven decisions, identify root causes, and address problems and opportunities.
Test assays on different lots of DNA and enzyme samples to evaluate batch‑to‑batch variability; identify causes for variability using the assay data.
Collaborate with operations and quality management team members; communicate effectively to accomplish project goals and milestones.
Work with cross‑functional teams such as Strain Engineering, Manufacturing, QA/QC; plan data review meetings, present results, suggest directions and next steps.
Requirements
B.S. in Chemical Engineering, Biochemistry or related field.
At least 24 months of experience (or equivalent role) in strain engineering, aseptic techniques, bacterial culture, extraction and characterization of DNA, UV/VIS absorbance, gel electrophoresis, sequencing data analysis.
12 months of experience as a research associate or similar role handling lab‑scale bioreactors for running fermentation and downstream processing equipment such as cell harvest by centrifugation, TFF, DNA column chromatography purification.
12 months of experience as a research associate or similar role handling analytical equipment such as HPLC to quantify DNA, RNA, organic acids.
12 months of experience working with electronic lab notebook, laboratory information management system and statistical software such as GMP.
Equal Employment Opportunity Statement Greenlight Biosciences Inc. provides equal employment opportunities to all employees and applicants for employment and prohibits discrimination and harassment of any type without regard to race, color, religion, age, sex, national origin, disability status, genetics, protected veteran status, sexual orientation, gender identity or expression, or any other characteristic protected by federal, state or local laws. This policy applies to all terms and conditions of employment, including recruiting, hiring, placement, promotion, termination, layoff, recall, transfer, leaves of absence, compensation, and training.
#J-18808-Ljbffr