Northwestern University
Research Technologist 3, Hepatology
Northwestern University, Chicago, Illinois, United States, 60290
Job Summary
Department:
MED-Hepatology;
Salary/Grade:
NEX/13.
Primary job responsibilities will entail using cell culture systems, including the development of stably transfected cell lines, to analyze protein and gene expression, transcriptional gene regulation and metabolic studies. In vivo murine investigations will involve library preparation, RNA‑seq, ChIP‑seq and ATAC‑seq using established dietary and genetically modified mouse models. Data analysis of experiments and a limited amount of laboratory management responsibilities will also be required.
Job Summary Participates in design and execution of research lab projects. Conducts experimental tests and procedures, processes and analyzes data, reviews and revises existing research methodologies, and may develop new methodologies, mathematical and/or computer models for predicting data. May ensure overall lab compliance with all associated rules and regulations.
Specific Responsibilities Molecular Biology
DNA, RNA, Protein isolation, purification and expression
Southern, Northern, Western blotting
PCR, RT‑PCR
Plasmid preparation
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Cellular Biology
Cell culture, cell isolation
Functional studies
Transfection
Histology
Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Immunohistochemistry
Microscopy
Immunology
Flow cytometry
ELISA, ELispot assay
Cytokine assay
Antibody production and purification
Animal
Husbandry
Breeding and screening transgenic mice
Dissections
Blood collection
Surgical procedures
Database management of colony
Miscellaneous Performs other duties as assigned.
Minimum Qualifications Successful completion of a full 4‑year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years' lab/bench or other relevant experience required; OR successful completion of a full course of study in an accredited college or university leading to a master's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 1 year lab/bench or other relevant experience.
Target hiring range for this position will be between $23.20 – 32.62 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.
Benefits At Northwestern, we are proud to provide meaningful, competitive, high‑quality health care plans, retirement benefits, tuition discounts and more! Visit us at
https://www.northwestern.edu/hr/benefits/index.html
to learn more.
Work‑Life and Wellness Northwestern offers comprehensive programs and services to help you and your family navigate life's challenges and opportunities, and adopt and maintain healthy lifestyles.
We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at
https://www.northwestern.edu/hr/benefits/work-life/index.html
to learn more.
Professional Growth & Development Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you're interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at
https://www.northwestern.edu/hr/learning/index.html
to learn more.
Equal Opportunity Employer Northwestern University is an Equal Opportunity Employer and does not discriminate on the basis of protected characteristics, including disability and veteran status. View Northwestern's non‑discrimination statement. Job applicants who wish to request an accommodation in the application or hiring process should contact the Office of Civil Rights and Title IX Compliance. View additional information on the accommodations process.
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MED-Hepatology;
Salary/Grade:
NEX/13.
Primary job responsibilities will entail using cell culture systems, including the development of stably transfected cell lines, to analyze protein and gene expression, transcriptional gene regulation and metabolic studies. In vivo murine investigations will involve library preparation, RNA‑seq, ChIP‑seq and ATAC‑seq using established dietary and genetically modified mouse models. Data analysis of experiments and a limited amount of laboratory management responsibilities will also be required.
Job Summary Participates in design and execution of research lab projects. Conducts experimental tests and procedures, processes and analyzes data, reviews and revises existing research methodologies, and may develop new methodologies, mathematical and/or computer models for predicting data. May ensure overall lab compliance with all associated rules and regulations.
Specific Responsibilities Molecular Biology
DNA, RNA, Protein isolation, purification and expression
Southern, Northern, Western blotting
PCR, RT‑PCR
Plasmid preparation
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Cellular Biology
Cell culture, cell isolation
Functional studies
Transfection
Histology
Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Immunohistochemistry
Microscopy
Immunology
Flow cytometry
ELISA, ELispot assay
Cytokine assay
Antibody production and purification
Animal
Husbandry
Breeding and screening transgenic mice
Dissections
Blood collection
Surgical procedures
Database management of colony
Miscellaneous Performs other duties as assigned.
Minimum Qualifications Successful completion of a full 4‑year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 3 years' lab/bench or other relevant experience required; OR successful completion of a full course of study in an accredited college or university leading to a master's or higher degree in a scientific major or related; OR appropriate combination of education and experience and 1 year lab/bench or other relevant experience.
Target hiring range for this position will be between $23.20 – 32.62 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.
Benefits At Northwestern, we are proud to provide meaningful, competitive, high‑quality health care plans, retirement benefits, tuition discounts and more! Visit us at
https://www.northwestern.edu/hr/benefits/index.html
to learn more.
Work‑Life and Wellness Northwestern offers comprehensive programs and services to help you and your family navigate life's challenges and opportunities, and adopt and maintain healthy lifestyles.
We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at
https://www.northwestern.edu/hr/benefits/work-life/index.html
to learn more.
Professional Growth & Development Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you're interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at
https://www.northwestern.edu/hr/learning/index.html
to learn more.
Equal Opportunity Employer Northwestern University is an Equal Opportunity Employer and does not discriminate on the basis of protected characteristics, including disability and veteran status. View Northwestern's non‑discrimination statement. Job applicants who wish to request an accommodation in the application or hiring process should contact the Office of Civil Rights and Title IX Compliance. View additional information on the accommodations process.
#J-18808-Ljbffr