BioLegend
Biochemist I - Recombinant Protein Manufacturing
BioLegend, San Diego, California, United States, 92189
Overview
BioLegend is seeking a Biochemist I to join our recombinant protein manufacturing team. The Biochemist I is responsible for producing recombinant protein products using bacterial cell culture systems, involving protein expression, purification, and quality support. Responsibilities include preparing buffer solutions, culture media, and dilution reagents; performing bacterial cell cultures; purifying recombinant proteins via affinity methods; and conducting DNA isolations following standard operating procedures. Additional duties may involve transferring products for downstream testing, maintaining production records and inventory, and preparing documentation related to products and projects. The ideal candidate is organized, collaborative, and possesses strong verbal and written communication skills and a solid understanding of recombinant protein manufacturing methodologies. Responsibilities
Perform DNA isolation, prepare various buffers and reagents for recombinant protein projects. Perform transformation, culture and harvest culture of bacterial cells. Clarify and process the culture supernatant/cell pellet/inclusion body to capture, extract, or denature/refold recombinant proteins. Purify recombinant proteins and antibodies by affinity chromatography. Monitor protein processing by SDS-PAGE for various production stages. Perform protein bulk processing (concentration, dilution, buffer change), purity analysis and endotoxin removal. Support inventory management of raw materials and plasmid constructions for protein production. Assist in transferring products across departments for QC or other inventory-related activities. Maintain batch records and documentation to comply with ISO13485:2016. Support the preparation of materials and verify that documents or historical records are available for incoming tissue and insect culture work orders. Support bottling of recombinant protein products. Maintain general upkeep of the lab. Other projects or responsibilities may be required. Minimum Qualifications – Education and Experience
Bachelor of Science in Life Science or related area with 6 months of working experience in a related biotech manufacturing environment. Preferred Qualifications – Education and Experience
Bachelor of Science in Life Science or related area with at least 12 months of working experience in a related biotech manufacturing environment. Experience in DNA isolation and bacterial culture. Experience in purification and formulation of recombinant protein. Applicants must be currently authorized to work in the United States on a full-time basis for any employer. Work Environment & Physical Demands
Lab. While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer, and occasional exposure to a warehouse environment. The physical demands described here are representative of those that must be met to perform the essential functions of this job. The employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear. The base salary range for this full-time position is $22.80 - $27.00/hr. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training. Your recruiter can share more about the specific salary range for your preferred location during the hiring process. Please note that base pay is only one part of our total compensation package and is determined within a range. This base pay range does not take into account bonuses, equity, or other benefits which may be applicable and are dependent on the level and position offered. What do we offer?
We provide competitive and comprehensive benefits to our employees. Highlights include: Medical, Dental, and Vision Insurance Options Life and Disability Insurance Paid Time-Off Parental Benefits Compassionate Care Leave 401k with Company Match Employee Stock Purchase Plan Learn more about Revvity’s benefits on our page. Log-in instructions are provided towards the bottom of the Bswift page. For benefit-eligible roles only. Part-time and temporary roles may not be eligible for all benefits listed. Please reach out to your recruiter for more information.
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BioLegend is seeking a Biochemist I to join our recombinant protein manufacturing team. The Biochemist I is responsible for producing recombinant protein products using bacterial cell culture systems, involving protein expression, purification, and quality support. Responsibilities include preparing buffer solutions, culture media, and dilution reagents; performing bacterial cell cultures; purifying recombinant proteins via affinity methods; and conducting DNA isolations following standard operating procedures. Additional duties may involve transferring products for downstream testing, maintaining production records and inventory, and preparing documentation related to products and projects. The ideal candidate is organized, collaborative, and possesses strong verbal and written communication skills and a solid understanding of recombinant protein manufacturing methodologies. Responsibilities
Perform DNA isolation, prepare various buffers and reagents for recombinant protein projects. Perform transformation, culture and harvest culture of bacterial cells. Clarify and process the culture supernatant/cell pellet/inclusion body to capture, extract, or denature/refold recombinant proteins. Purify recombinant proteins and antibodies by affinity chromatography. Monitor protein processing by SDS-PAGE for various production stages. Perform protein bulk processing (concentration, dilution, buffer change), purity analysis and endotoxin removal. Support inventory management of raw materials and plasmid constructions for protein production. Assist in transferring products across departments for QC or other inventory-related activities. Maintain batch records and documentation to comply with ISO13485:2016. Support the preparation of materials and verify that documents or historical records are available for incoming tissue and insect culture work orders. Support bottling of recombinant protein products. Maintain general upkeep of the lab. Other projects or responsibilities may be required. Minimum Qualifications – Education and Experience
Bachelor of Science in Life Science or related area with 6 months of working experience in a related biotech manufacturing environment. Preferred Qualifications – Education and Experience
Bachelor of Science in Life Science or related area with at least 12 months of working experience in a related biotech manufacturing environment. Experience in DNA isolation and bacterial culture. Experience in purification and formulation of recombinant protein. Applicants must be currently authorized to work in the United States on a full-time basis for any employer. Work Environment & Physical Demands
Lab. While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer, and occasional exposure to a warehouse environment. The physical demands described here are representative of those that must be met to perform the essential functions of this job. The employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear. The base salary range for this full-time position is $22.80 - $27.00/hr. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training. Your recruiter can share more about the specific salary range for your preferred location during the hiring process. Please note that base pay is only one part of our total compensation package and is determined within a range. This base pay range does not take into account bonuses, equity, or other benefits which may be applicable and are dependent on the level and position offered. What do we offer?
We provide competitive and comprehensive benefits to our employees. Highlights include: Medical, Dental, and Vision Insurance Options Life and Disability Insurance Paid Time-Off Parental Benefits Compassionate Care Leave 401k with Company Match Employee Stock Purchase Plan Learn more about Revvity’s benefits on our page. Log-in instructions are provided towards the bottom of the Bswift page. For benefit-eligible roles only. Part-time and temporary roles may not be eligible for all benefits listed. Please reach out to your recruiter for more information.
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