Revvity
Biochemist I - Recombinant Protein Manufacturing
Revvity, San Diego, California, United States, 92189
Job Summary
BioLegend is seeking a Biochemist I join our recombinant protein manufacturing team. The Biochemist I is responsible for producing recombinant protein products using bacterial cell culture systems. This role involves multiple stages of production, including protein expression, purification, and quality support. Key responsibilities include preparing buffer solutions, culture media, and dilution reagents; performing bacterial cell cultures; purifying recombinant proteins via affinity methods; and conducting DNA isolations following standard operating procedures.
Additional duties may involve transferring products for downstream testing, maintaining production records and inventory, and preparing documentation related to products and projects. The ideal candidate is an organized, collaborative team player with strong verbal and written communication skills and a solid understanding of recombinant protein manufacturing methodologies.
Essential Functions
Perform DNA isolation, prepare various buffers and reagents for experiment of recombinant protein projects.
Perform transformation, culture and harvest culture of bacterial cells.
Clarify and process the culture supernatant/cell pellet/inclusion body to capture, extract, or denature/refold recombinant proteins.
Purify of recombinant proteins & antibodies by affinity chromatography.
Monitor protein processing by SDS-PAGE for various protein production stages.
Perform protein bulk processing (concentration, dilution, buffer change), purity analysis and endotoxin removal.
Support inventory management of raw material and plasmid constructions for protein production.
Assist in transferring products across different departments for QC or other inventory-related activities.
Maintain batch records and other documentations that are essential to be in compliance with ISO13485:2016.
Support the preparation of material and checking for documents or historical records are available for incoming tissue and insect culture work orders.
Support bottling of recombinant protein products.
Maintain general upkeep of the lab.
Other projects or responsibilities may be required.
Minimum Qualifications – Education And Experience
Bachelor of Science in Life Science or related area with 6 months of working experience in related biotech manufacturing environment.
Preferred Qualifications – Education And Experience
Bachelor of Science in Life Science or related area with at least 12 months of working experience in related biotech manufacturing environment.
Experience in DNA isolation and bacterial culture.
Experienced in purification and formulation of recombinant protein.
Work Environment & Physical Demands Lab. While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.
Physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.
Compensation The base salary range for this full-time position is $22.80 - $27.00/hr. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training.
Benefits
Medical, Dental, and Vision Insurance Options
Life and Disability Insurance
Paid Time-Off
Parental Benefits
Compassionate Care Leave
401k with Company Match
Employee Stock Purchase Plan
EEO & Eligibility Applicants must be currently authorized to work in the United States on a full-time basis for any employer.
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Additional duties may involve transferring products for downstream testing, maintaining production records and inventory, and preparing documentation related to products and projects. The ideal candidate is an organized, collaborative team player with strong verbal and written communication skills and a solid understanding of recombinant protein manufacturing methodologies.
Essential Functions
Perform DNA isolation, prepare various buffers and reagents for experiment of recombinant protein projects.
Perform transformation, culture and harvest culture of bacterial cells.
Clarify and process the culture supernatant/cell pellet/inclusion body to capture, extract, or denature/refold recombinant proteins.
Purify of recombinant proteins & antibodies by affinity chromatography.
Monitor protein processing by SDS-PAGE for various protein production stages.
Perform protein bulk processing (concentration, dilution, buffer change), purity analysis and endotoxin removal.
Support inventory management of raw material and plasmid constructions for protein production.
Assist in transferring products across different departments for QC or other inventory-related activities.
Maintain batch records and other documentations that are essential to be in compliance with ISO13485:2016.
Support the preparation of material and checking for documents or historical records are available for incoming tissue and insect culture work orders.
Support bottling of recombinant protein products.
Maintain general upkeep of the lab.
Other projects or responsibilities may be required.
Minimum Qualifications – Education And Experience
Bachelor of Science in Life Science or related area with 6 months of working experience in related biotech manufacturing environment.
Preferred Qualifications – Education And Experience
Bachelor of Science in Life Science or related area with at least 12 months of working experience in related biotech manufacturing environment.
Experience in DNA isolation and bacterial culture.
Experienced in purification and formulation of recombinant protein.
Work Environment & Physical Demands Lab. While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.
Physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.
Compensation The base salary range for this full-time position is $22.80 - $27.00/hr. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training.
Benefits
Medical, Dental, and Vision Insurance Options
Life and Disability Insurance
Paid Time-Off
Parental Benefits
Compassionate Care Leave
401k with Company Match
Employee Stock Purchase Plan
EEO & Eligibility Applicants must be currently authorized to work in the United States on a full-time basis for any employer.
#J-18808-Ljbffr