St. Jude Children's Research Hospital
Cell Assay Lead/Senior Scientist - Blue Sky Kinases
St. Jude Children's Research Hospital, Memphis, Tennessee, us, 37544
We are looking for an experienced cell biologist to participate in our exciting studies of the human protein kinome. This Lead/Senior Scientist position is part of the Blue Sky Initiative “Seeing the Invisible in Protein Kinases” at St. Jude Children’s Research Hospital in Memphis, TN.
Overview As a member of the Cell-Based Assays team, the candidate will use their molecular biology, biochemistry, and cell culture skills to perform research that translates novel insights regarding kinase structure and regulation in vitro into functional effects in cells. Additionally, the candidate will assist in performing cell-based analysis in high-, medium-, and low-throughput platforms of candidate inhibitors of kinases. The work is diverse providing an opportunity to study multiple kinases, perform appropriate background research, optimize cell-based assays of various types using many different cell lines or primary cells. Not only are the cell-based studies used to validate the structural findings, but they are also used to discover new kinase-mediated biology or new mechanisms of kinase regulation.
Blue Sky Initiative “Seeing the Invisible in Protein Kinases” is an inter‑disciplinary project in the Department of Structural Biology and is supported by US $50 million in institutional funding. This large‑scale project leverages ultra‑high field nuclear magnetic resonance (NMR), complemented with cryoEM, X‑ray crystallography, data‑science and computational approaches, to characterize the conformational landscape of the human kinome. Knowledge of the conformations of kinases is then used to discover new biology regarding kinase function and regulation in cells, understand the structural and functional consequences of disease‑associated mutations, and identify novel therapeutic opportunities.
Responsibilities
Research kinase‑mediated signaling pathways, kinase targets or kinase‑dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects.
Identify appropriate cellular assays and cells to perform structure‑guided analysis of kinase signaling output and cellular phenotypes related to structure‑guided perturbations in kinase regulation or function.
Develop strategies and plans for implementing cell‑based assays, including but not limited to evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT‑PCR), protein ubiquitination, and protein abundance.
Develop strategies and plans for implementing cell‑based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation.
Perform optimization and troubleshooting of cell‑based assays.
Perform statistical analysis using graphing software or R scripts.
Interpret, analyze, and communicate cell‑based assay results within the Cell‑Based Assays team and with collaborators on specific kinase projects.
Experience and Expertise
Experience in analysis of proteinkinases and phosphorylation‑dependent signaling events or pathways.
Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells.
Expertise in multiple methods for exogenous protein expression.
Proficiency in presentation of experimental design and results using PowerPoint or similar software.
Experience with mouse models, preferred but not essential.
Technical Capabilities
Cloning, PCR, and qRT‑PCR
Sterile technique
Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation
Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery
Immunoprecipitation and Western blotting
Protein‑protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays
Flow cytometry and fluorescence‑activated cell sorting (FACS)
Reporter gene assays
Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony‑forming, autophagy, endocytosis, secretion, or differentiation assays
Microscopy, such as immunofluorescence, live‑cell imaging, subcellular localization, colocalization analysis
Cellular fractionation
Competencies
Creative and results‑driven with strong critical thinking and the ability to learn quickly
Hardworking, well‑organized, flexible, and capable of multitasking efficiently in a fast‑paced environment
Collaborative and efficient member of teams with diverse backgrounds and opinions
Ability to prioritize work and complete assignments promptly
Minimum Education and Experience for either level
Bachelor's degree in relevant scientific area is required.
Minimum Experience for Senior Scientist
Ten (10) years relevant work experience is required with a Bachelor's degree.
Eight (8) years of relevant work experience is required with a Master's degree.
Five (5) years of relevant work experience is required with a PhD.
Minimum Experience for Lead Scientist
Twelve (12) years of relevant work experience is required with a Bachelor's degree.
Ten (10) years of relevant experience is required with a Master's degree.
Seven (7) years of relevant work experience is required with a PhD.
A reasonable estimate of the current salary range is $104,000 - $186,160 per year for the role of Senior Scientist, $125,840 - $238,160 per year for the role of Lead Scientist.
Benefits
World‑class facilities
State‑of‑the‑art technologies
Extraordinary support
A collaborative, bench‑to‑bedside environment
You will see, firsthand, how your science translates into survival for the children that we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.
Better at Work. Better at Life. When you work at St. Jude, you’ll join a highly collaborative work culture that inspires you every day to be your best. With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world‑class institution. This includes work‑life balance with generous paid time‑off and on‑campus conveniences that make life a little easier. Join us and you’ll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a “Best Place to Work.”
Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $125,840 - $238,160 per year for the role of Cell Assay Lead/Senior Scientist - Blue Sky Kinases.
Explore our exceptional benefits!
St. Jude is an Equal Opportunity Employer.
No Search Firms St. Jude Children’s Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.
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Overview As a member of the Cell-Based Assays team, the candidate will use their molecular biology, biochemistry, and cell culture skills to perform research that translates novel insights regarding kinase structure and regulation in vitro into functional effects in cells. Additionally, the candidate will assist in performing cell-based analysis in high-, medium-, and low-throughput platforms of candidate inhibitors of kinases. The work is diverse providing an opportunity to study multiple kinases, perform appropriate background research, optimize cell-based assays of various types using many different cell lines or primary cells. Not only are the cell-based studies used to validate the structural findings, but they are also used to discover new kinase-mediated biology or new mechanisms of kinase regulation.
Blue Sky Initiative “Seeing the Invisible in Protein Kinases” is an inter‑disciplinary project in the Department of Structural Biology and is supported by US $50 million in institutional funding. This large‑scale project leverages ultra‑high field nuclear magnetic resonance (NMR), complemented with cryoEM, X‑ray crystallography, data‑science and computational approaches, to characterize the conformational landscape of the human kinome. Knowledge of the conformations of kinases is then used to discover new biology regarding kinase function and regulation in cells, understand the structural and functional consequences of disease‑associated mutations, and identify novel therapeutic opportunities.
Responsibilities
Research kinase‑mediated signaling pathways, kinase targets or kinase‑dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects.
Identify appropriate cellular assays and cells to perform structure‑guided analysis of kinase signaling output and cellular phenotypes related to structure‑guided perturbations in kinase regulation or function.
Develop strategies and plans for implementing cell‑based assays, including but not limited to evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT‑PCR), protein ubiquitination, and protein abundance.
Develop strategies and plans for implementing cell‑based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation.
Perform optimization and troubleshooting of cell‑based assays.
Perform statistical analysis using graphing software or R scripts.
Interpret, analyze, and communicate cell‑based assay results within the Cell‑Based Assays team and with collaborators on specific kinase projects.
Experience and Expertise
Experience in analysis of proteinkinases and phosphorylation‑dependent signaling events or pathways.
Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells.
Expertise in multiple methods for exogenous protein expression.
Proficiency in presentation of experimental design and results using PowerPoint or similar software.
Experience with mouse models, preferred but not essential.
Technical Capabilities
Cloning, PCR, and qRT‑PCR
Sterile technique
Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation
Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery
Immunoprecipitation and Western blotting
Protein‑protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays
Flow cytometry and fluorescence‑activated cell sorting (FACS)
Reporter gene assays
Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony‑forming, autophagy, endocytosis, secretion, or differentiation assays
Microscopy, such as immunofluorescence, live‑cell imaging, subcellular localization, colocalization analysis
Cellular fractionation
Competencies
Creative and results‑driven with strong critical thinking and the ability to learn quickly
Hardworking, well‑organized, flexible, and capable of multitasking efficiently in a fast‑paced environment
Collaborative and efficient member of teams with diverse backgrounds and opinions
Ability to prioritize work and complete assignments promptly
Minimum Education and Experience for either level
Bachelor's degree in relevant scientific area is required.
Minimum Experience for Senior Scientist
Ten (10) years relevant work experience is required with a Bachelor's degree.
Eight (8) years of relevant work experience is required with a Master's degree.
Five (5) years of relevant work experience is required with a PhD.
Minimum Experience for Lead Scientist
Twelve (12) years of relevant work experience is required with a Bachelor's degree.
Ten (10) years of relevant experience is required with a Master's degree.
Seven (7) years of relevant work experience is required with a PhD.
A reasonable estimate of the current salary range is $104,000 - $186,160 per year for the role of Senior Scientist, $125,840 - $238,160 per year for the role of Lead Scientist.
Benefits
World‑class facilities
State‑of‑the‑art technologies
Extraordinary support
A collaborative, bench‑to‑bedside environment
You will see, firsthand, how your science translates into survival for the children that we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.
Better at Work. Better at Life. When you work at St. Jude, you’ll join a highly collaborative work culture that inspires you every day to be your best. With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world‑class institution. This includes work‑life balance with generous paid time‑off and on‑campus conveniences that make life a little easier. Join us and you’ll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a “Best Place to Work.”
Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $125,840 - $238,160 per year for the role of Cell Assay Lead/Senior Scientist - Blue Sky Kinases.
Explore our exceptional benefits!
St. Jude is an Equal Opportunity Employer.
No Search Firms St. Jude Children’s Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.
#J-18808-Ljbffr