Biologist (Boston)

We are seeking a highly skilled, passionate, and motivated scientist to join our diabetes research team. This position will play a key lab-based role in advancing iPSC stem cell engineering and contribute to the development of the next generation of islet cell therapies. Responsibilities: Enable and advance iPSC gene editing, cell line development, and evaluation of differentiated cells. Perform routine iPSC cell culture, passaging, and media preparation. Optimize workflows for iPSC CRISPR-based gene editing, gRNA screening, and delivery. Develop and perform assays for on-/off-target analysis using PCR and NGS workflows. Generate clonal gene-edited iPSC lines and characterize them via flow cytometry, ddPCR, dPCR, and qPCR. Culture and execute routine directed differentiation of human iPSCs to pancreatic islets, performing assessments such as cell counts, viability, and metabolite analysis. Assist scientists in evaluating and designing mitigation strategies to ensure genomic stability of iPSC lines and drug substances. Collaborate cross-functionally to support the transfer of R&D assays to GMP environments. Maintain accurate and detailed experimental records, synthesize results, prepare reports, and present findings in meetings. Contribute to the development and refinement of SOPs. Execute work plans efficiently and meet established deadlines. Qualifications: BS or MS in Stem Cell Biology, Cell Biology, Bioengineering, or a related field. 3+ years of relevant industry experience for MS candidates, or 5+ years for BS candidates. Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques. Proficiency in gene editing and cell line development, including protocol optimization and flow-based sorting. Hands-on experience with delivery methods for gene editing tools. Strong communication and interpersonal skills, with the ability to work effectively in a collaborative team environment. Excellent organizational skills and attention to detail in record keeping. Experience in pancreatic islet directed differentiation is highly desirable. Experience in transitioning research-grade processes to GMP-grade is highly desirable.