Integrated Resources, Inc ( IRI )
Overview
This range is provided by Integrated Resources, Inc (IRI). Your actual pay will be based on your skills and experience — talk with your recruiter to learn more. Base pay range $50.00/hr - $55.00/hr Direct message the job poster from Integrated Resources, Inc (IRI) Job title
Recruitment Manager | Driving Pharma & Biotech Hiring Success | Talent Strategy & Leadership. About the Company : We are seeking a highly skilled, passionate and motivated scientist to join our diabetes team. This position will play a key lab-based role in advancing iPSC stem cell engineering at Client and will contribute to the development of the next generation of islet cell therapies. About the Role : Successful candidates will have experience with human iPSC cell culture, differentiation, banking, gene editing, molecular biology techniques and assay development. This position will report to Director – Islet Cell Therapy, Stem Cell Biology and Cell Engineering. Responsibilities
Enabling and advancing iPSC gene editing, cell line development and evaluation of differentiated cells. Routine iPSC cell culture, passaging and media making. Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening and delivery. Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows. Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR. Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, metabolite assessments. Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance. Collaborate cross-functionally to support transfer of R&D assays to GMP. Maintain accurate and detailed records of all experiments, synthesize results, prepare reports and present in meetings. Contribute to SOP development. Work seamlessly in executing workplans and meeting deadlines. Qualifications
BS/MS in Stem Cell Biology, Cell Biology, Bioengineering or a related field of relevant industry experience in gene editing and cell therapy. 3+ years for MS 5+ years for BS Required Skills
Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques. Proficiency in gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting. Hands-on experience with cell delivery methods of gene editing tools. Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment. Strong organizational skills and diligent record keeping. Preferred Skills
Experience in pancreatic islet directed differentiation is highly desirable. Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable. Seniority level
Mid-Senior level Employment type
Contract Job function
Science Industries
Pharmaceutical Manufacturing and Biotechnology Research
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This range is provided by Integrated Resources, Inc (IRI). Your actual pay will be based on your skills and experience — talk with your recruiter to learn more. Base pay range $50.00/hr - $55.00/hr Direct message the job poster from Integrated Resources, Inc (IRI) Job title
Recruitment Manager | Driving Pharma & Biotech Hiring Success | Talent Strategy & Leadership. About the Company : We are seeking a highly skilled, passionate and motivated scientist to join our diabetes team. This position will play a key lab-based role in advancing iPSC stem cell engineering at Client and will contribute to the development of the next generation of islet cell therapies. About the Role : Successful candidates will have experience with human iPSC cell culture, differentiation, banking, gene editing, molecular biology techniques and assay development. This position will report to Director – Islet Cell Therapy, Stem Cell Biology and Cell Engineering. Responsibilities
Enabling and advancing iPSC gene editing, cell line development and evaluation of differentiated cells. Routine iPSC cell culture, passaging and media making. Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening and delivery. Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows. Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR. Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, metabolite assessments. Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance. Collaborate cross-functionally to support transfer of R&D assays to GMP. Maintain accurate and detailed records of all experiments, synthesize results, prepare reports and present in meetings. Contribute to SOP development. Work seamlessly in executing workplans and meeting deadlines. Qualifications
BS/MS in Stem Cell Biology, Cell Biology, Bioengineering or a related field of relevant industry experience in gene editing and cell therapy. 3+ years for MS 5+ years for BS Required Skills
Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques. Proficiency in gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting. Hands-on experience with cell delivery methods of gene editing tools. Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment. Strong organizational skills and diligent record keeping. Preferred Skills
Experience in pancreatic islet directed differentiation is highly desirable. Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable. Seniority level
Mid-Senior level Employment type
Contract Job function
Science Industries
Pharmaceutical Manufacturing and Biotechnology Research
#J-18808-Ljbffr