TechDigital Group
Sr. Associate Scientist or Scientist, RNA Process Development
TechDigital Group, Seattle, Washington, us, 98127
Sr. Associate Scientist or Scientist, RNA Process Development
Job Title– Sr. Associate Scientist or Scientist, RNA Process Development Location: Seattle, WA * 100% Onsite Top Skills: Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE) Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity Experience working with, storing, and analyzing purified nucleic acids and proteins Experience with optimizing molecular cloning reactions Experience with gene editing primary human cells or immortalized cell-lines Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt). A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment. Key Responsibilities: Design and execute nucleic acid ligation reactions using "splint ligation" methodology Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods Perform purification of oligonucleotides using chromatography and/or preparative PAGE Design and evaluate the performance of new biophysical assays to identify and assess CQA's Manage samples and data related to gene editing platform process development Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.) Actively participate and support lab maintenance and continuous improvement initiatives Maintain accurate and detailed laboratory notebook and documentation Summarize and present data in group meetings and contribute to group discussions Author technical reports, study memos, and protocols Basic Qualifications: STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE) Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity Experience working with, storing, and analyzing purified nucleic acids and proteins Independently motivated with the ability to work in teams Ability to communicate clearly and concisely through oral presentation and technical writing Ability to leverage literature, internal and external resources to solve problems and develop new technologies Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment Preferred Qualifications: Degree in biological or chemical engineering, biochemistry, or bio-related field Experience with optimizing molecular cloning reactions Experience with gene editing primary human cells or immortalized cell-lines Experience with solid-phase oligonucleotide synthesis Experience with bioinformatic tools used to predict RNA secondary structure Experience with JMP for DoE design and analysis
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Job Title– Sr. Associate Scientist or Scientist, RNA Process Development Location: Seattle, WA * 100% Onsite Top Skills: Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE) Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity Experience working with, storing, and analyzing purified nucleic acids and proteins Experience with optimizing molecular cloning reactions Experience with gene editing primary human cells or immortalized cell-lines Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt). A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment. Key Responsibilities: Design and execute nucleic acid ligation reactions using "splint ligation" methodology Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods Perform purification of oligonucleotides using chromatography and/or preparative PAGE Design and evaluate the performance of new biophysical assays to identify and assess CQA's Manage samples and data related to gene editing platform process development Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.) Actively participate and support lab maintenance and continuous improvement initiatives Maintain accurate and detailed laboratory notebook and documentation Summarize and present data in group meetings and contribute to group discussions Author technical reports, study memos, and protocols Basic Qualifications: STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE) Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity Experience working with, storing, and analyzing purified nucleic acids and proteins Independently motivated with the ability to work in teams Ability to communicate clearly and concisely through oral presentation and technical writing Ability to leverage literature, internal and external resources to solve problems and develop new technologies Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment Preferred Qualifications: Degree in biological or chemical engineering, biochemistry, or bio-related field Experience with optimizing molecular cloning reactions Experience with gene editing primary human cells or immortalized cell-lines Experience with solid-phase oligonucleotide synthesis Experience with bioinformatic tools used to predict RNA secondary structure Experience with JMP for DoE design and analysis
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