Eli Lilly
Advisor - Scientific Lead, Cell Line Generation
Eli Lilly, Boulder, Colorado, United States, 80302
Laboratory Scientist
At Lilly, we unite caring with discovery to make life better for people around the world. We are a global healthcare leader headquartered in Indianapolis, Indiana. Our employees around the world work to discover and bring life-changing medicines to those who need them, improve the understanding and management of disease, and give back to our communities through philanthropy and volunteerism. We give our best effort to our work, and we put people first. We're looking for people who are determined to make life better for people around the world. At Lilly, we serve an extraordinary purpose. We make a difference for people around the globe by discovering, developing and delivering medicines that help them live longer, healthier, more active lives. Not only do we deliver breakthrough medications, but you also can count on us to develop creative solutions to support communities through philanthropy and volunteerism. Position Summary: We are looking for a highly capable and motivated laboratory-based scientist experienced in cell line generation and characterization. This individual will be a part of the cell line engineering/cell biology team that generates engineered cell lines for our preclinical programs in oncology. The primary responsibilities will include engineering cell lines for use in in vitro mechanistic assays and studies. The right candidate is expected to work within a collaborative team environment, while being able to work independently to design, execute, and optimize experiments. Beyond performing experiments, the scientist will analyze the data from experiments, present the data in meetings and work with the rest of the team to determine the next steps. Position level will be commensurate with applicant's experience. Roles and Responsibilities of the Position: Engineer various cell lines for pre-clinical studies such as over-expression, genome-editing, reporter lines etc. Maintain cultures of frequently used suspension and adherent cell lines. Deposit and archive cell lines in the company's repository cell line bank. Keep detailed and accurate laboratory records, present results at group meetings and project team meetings. Viral construct cloning, viral titer measurement, and viral stock maintenance. Perform routine molecular and cell biology techniques like transfection/electroporation, DNA/RNA/protein extraction, qPCR analysis, western blot analysis, flow cytometry, fluorescent microscopy and so on. Basic Requirements: PhD in biology, cell biology, molecular biology or a related field and 0-5 years experiences; or MS in biology, cell biology, molecular biology or a related field and 5-8 years experiences. Prior cell culture experience with immortalized cell lines, cancer cell lines and/or primary cells. Must be organized and efficient and able to work according to timelines. Additional Preferences: Experience creating stable cell lines, transient transfections, viral transductions. Genome editing experience would be a plus. Experience with performing flow cytometry, cell sorting, qPCR, ELISA, western blot, and fluorescent microscopy, all in the context of mammalian cells. A high level of proficiency with many kinds of scientific software used to operate lab equipment and/or the ability to quickly master new software. Experience and proficiency with data analysis using statistical software such as Excel, Prism, Spotfire or others. Pharma/biotech drug discovery experience a plus. Physical Demands/ Travel: The physical demands of this job are consistent with laboratory bench setting and light office duties. Work Environment: This position's work environment is in a laboratory-based setting.
At Lilly, we unite caring with discovery to make life better for people around the world. We are a global healthcare leader headquartered in Indianapolis, Indiana. Our employees around the world work to discover and bring life-changing medicines to those who need them, improve the understanding and management of disease, and give back to our communities through philanthropy and volunteerism. We give our best effort to our work, and we put people first. We're looking for people who are determined to make life better for people around the world. At Lilly, we serve an extraordinary purpose. We make a difference for people around the globe by discovering, developing and delivering medicines that help them live longer, healthier, more active lives. Not only do we deliver breakthrough medications, but you also can count on us to develop creative solutions to support communities through philanthropy and volunteerism. Position Summary: We are looking for a highly capable and motivated laboratory-based scientist experienced in cell line generation and characterization. This individual will be a part of the cell line engineering/cell biology team that generates engineered cell lines for our preclinical programs in oncology. The primary responsibilities will include engineering cell lines for use in in vitro mechanistic assays and studies. The right candidate is expected to work within a collaborative team environment, while being able to work independently to design, execute, and optimize experiments. Beyond performing experiments, the scientist will analyze the data from experiments, present the data in meetings and work with the rest of the team to determine the next steps. Position level will be commensurate with applicant's experience. Roles and Responsibilities of the Position: Engineer various cell lines for pre-clinical studies such as over-expression, genome-editing, reporter lines etc. Maintain cultures of frequently used suspension and adherent cell lines. Deposit and archive cell lines in the company's repository cell line bank. Keep detailed and accurate laboratory records, present results at group meetings and project team meetings. Viral construct cloning, viral titer measurement, and viral stock maintenance. Perform routine molecular and cell biology techniques like transfection/electroporation, DNA/RNA/protein extraction, qPCR analysis, western blot analysis, flow cytometry, fluorescent microscopy and so on. Basic Requirements: PhD in biology, cell biology, molecular biology or a related field and 0-5 years experiences; or MS in biology, cell biology, molecular biology or a related field and 5-8 years experiences. Prior cell culture experience with immortalized cell lines, cancer cell lines and/or primary cells. Must be organized and efficient and able to work according to timelines. Additional Preferences: Experience creating stable cell lines, transient transfections, viral transductions. Genome editing experience would be a plus. Experience with performing flow cytometry, cell sorting, qPCR, ELISA, western blot, and fluorescent microscopy, all in the context of mammalian cells. A high level of proficiency with many kinds of scientific software used to operate lab equipment and/or the ability to quickly master new software. Experience and proficiency with data analysis using statistical software such as Excel, Prism, Spotfire or others. Pharma/biotech drug discovery experience a plus. Physical Demands/ Travel: The physical demands of this job are consistent with laboratory bench setting and light office duties. Work Environment: This position's work environment is in a laboratory-based setting.