Oregon Health & Science University (OHSU)
Research Assistant 2 -OR-Senior Research Assistant
Oregon Health & Science University (OHSU), Portland, Oregon, United States, 97201
Research Assistant Position
Sarah Andres' Lab is located within the Department of Pediatrics. The Andres Lab investigates intestinal epithelial differentiation, nutrient handling, and barrier function with a focus on the neonatal inflammatory disease necrotizing enterocolitis (NEC) and the use of human milk extracellular vesicles as a potential therapeutic. By using rare human patient samples, genetic mouse models, and cutting-edge patient-derived organoids, the Andres Lab is at the forefront of research in these fields evidenced by high impact publications and multiple funding sources. We are currently recruiting for a Research Assistant 2 -OR- Senior Research Assistant depending on qualifications. Function/Duties of Position
The Research Assistant is responsible for executing complex organoid experiments and helping to develop functional assays to delineate mechanisms of human milk action on intestinal development and maturation. This involves generating neonatal patient-derived intestinal organoids (enteroids) from surgical tissue samples. Cultivating enteroids requires keen attention to detail and impeccable sterile technique. The Research Assistant is also responsible for isolating human milk extracellular vesicles via density-gradient ultracentrifugation, quantifying by Nanoparticle Tracking analysis, and running various downstream analyses or experiments including, qPCR, western blotting, immunostaining, imaging and cell uptake studies, microRNA sequencing, proteomics, and oral gavage into rodent models. Experiments are currently focused on nutrient uptake, lipid handling, and metabolism. The ability to manage multiple co-occurring experiments involving enteroids and/or mice is important. In addition to primary project responsibilities, this position will assist lab team members with experiments involving neonatal mouse pups, oral gavage, and use of a hypoxic chamber. This also includes intestinal tissue harvest and processing, enteroid generation and propagation, immune cell isolation and culture, immunostaining and microscopy, and molecular biology. In addition to research skills training, our team prioritizes mentorship and training in career development. Required Qualifications
Required qualifications for Research Assistant 2: Bachelor's in relevant field OR Associate's AND 3 years of relevant experience OR 4 years of relevant experience OR Equivalent combination of training and experience. 1+ years in a wet bench laboratory. Required qualifications for Senior Research Assistant: Bachelor's in relevant field AND 1 years of relevant experience OR Equivalent combination of training and experience. Wet bench laboratory experience. Job Related Knowledge, Skills and Abilities: Working as part of a team Working independently Working in dynamic, fast paced environment Ability to juggle multiple tasks Ability to master detail-oriented techniques and procedures Computer skills Must have excellent communication, analytical and organizational skills: both written and verbal Experience in cell culture Experience in tissue handling Experience in molecular biology techniques Ability to multi-task Flexible Strong problem-solving skills Preferred Qualifications
Bachelor's degree in biologic sciences 3+ years of Wet bench laboratory experience Basic laboratory experience Animal handling experience Laboratory/cell/organoid culture experience Cell culture techniques Molecular biology skills Bioinformatic experience Extracellular vesicle biology Metabolism or metabolic studies experience Microscopy Immunostaining Mouse handling experience Additional Details
***Upload cover letter and resume. Please be sure to include months and years (MM/YYYY format) to the resume for jobs/experience. All Are Welcome
Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.
Sarah Andres' Lab is located within the Department of Pediatrics. The Andres Lab investigates intestinal epithelial differentiation, nutrient handling, and barrier function with a focus on the neonatal inflammatory disease necrotizing enterocolitis (NEC) and the use of human milk extracellular vesicles as a potential therapeutic. By using rare human patient samples, genetic mouse models, and cutting-edge patient-derived organoids, the Andres Lab is at the forefront of research in these fields evidenced by high impact publications and multiple funding sources. We are currently recruiting for a Research Assistant 2 -OR- Senior Research Assistant depending on qualifications. Function/Duties of Position
The Research Assistant is responsible for executing complex organoid experiments and helping to develop functional assays to delineate mechanisms of human milk action on intestinal development and maturation. This involves generating neonatal patient-derived intestinal organoids (enteroids) from surgical tissue samples. Cultivating enteroids requires keen attention to detail and impeccable sterile technique. The Research Assistant is also responsible for isolating human milk extracellular vesicles via density-gradient ultracentrifugation, quantifying by Nanoparticle Tracking analysis, and running various downstream analyses or experiments including, qPCR, western blotting, immunostaining, imaging and cell uptake studies, microRNA sequencing, proteomics, and oral gavage into rodent models. Experiments are currently focused on nutrient uptake, lipid handling, and metabolism. The ability to manage multiple co-occurring experiments involving enteroids and/or mice is important. In addition to primary project responsibilities, this position will assist lab team members with experiments involving neonatal mouse pups, oral gavage, and use of a hypoxic chamber. This also includes intestinal tissue harvest and processing, enteroid generation and propagation, immune cell isolation and culture, immunostaining and microscopy, and molecular biology. In addition to research skills training, our team prioritizes mentorship and training in career development. Required Qualifications
Required qualifications for Research Assistant 2: Bachelor's in relevant field OR Associate's AND 3 years of relevant experience OR 4 years of relevant experience OR Equivalent combination of training and experience. 1+ years in a wet bench laboratory. Required qualifications for Senior Research Assistant: Bachelor's in relevant field AND 1 years of relevant experience OR Equivalent combination of training and experience. Wet bench laboratory experience. Job Related Knowledge, Skills and Abilities: Working as part of a team Working independently Working in dynamic, fast paced environment Ability to juggle multiple tasks Ability to master detail-oriented techniques and procedures Computer skills Must have excellent communication, analytical and organizational skills: both written and verbal Experience in cell culture Experience in tissue handling Experience in molecular biology techniques Ability to multi-task Flexible Strong problem-solving skills Preferred Qualifications
Bachelor's degree in biologic sciences 3+ years of Wet bench laboratory experience Basic laboratory experience Animal handling experience Laboratory/cell/organoid culture experience Cell culture techniques Molecular biology skills Bioinformatic experience Extracellular vesicle biology Metabolism or metabolic studies experience Microscopy Immunostaining Mouse handling experience Additional Details
***Upload cover letter and resume. Please be sure to include months and years (MM/YYYY format) to the resume for jobs/experience. All Are Welcome
Oregon Health & Science University values a diverse and culturally competent workforce. We are proud of our commitment to being an equal opportunity, affirmative action organization that does not discriminate against applicants on the basis of any protected class status, including disability status and protected veteran status. Individuals with diverse backgrounds and those who promote diversity and a culture of inclusion are encouraged to apply. To request reasonable accommodation contact the Affirmative Action and Equal Opportunity Department at 503-494-5148 or aaeo@ohsu.edu.