Biologist

Job Title Biologist Location: Boston, MA Work schedule: Mon- Fri 8am- 5pm Type: Contract Requirements B.S. and/or M.S. in biology or a closely related field with 3+ years of laboratory experience. A background in the biology and physiology of energy balance regulation and metabolic disease is a plus. Familiar with Sanger sequencing analysis (ICE) Experience with immunohistochemistry, in situ hybridization (RNAscope), various assay detection technologies (fluorescence, luminescence, assays) and ability to implement and validate new assay technologies or technologies. Experience with imaging techniques (confocal microscopy) Brain tissue sectioning and histology techniques Demonstrated ability to work on cross-functional teams and projects. Strong communication skills and experience presenting data in a team environment Responsibilities Aseptic cell culture techniques with mammalian cells. Transient transfections and maintain cell lines Development of cell-based functional assays Perform molecular and biochemical assessments, including protein and RNA detection and Sanger sequencing, from ex vivo samples collected from bulk tissues or cells Gene expression studies by qPCR, protein analysis by western blot, and ELISA Knowledge of basic pharmacology (e.g., concentration/response curves, determination of binding affinity, potency, Hill’s slope, and in vitro efficacy). Data analysis skills, including PRISM Benefits System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan. Ref: #558-Scientific