CEDARS-SINAI
Biomedical Specialist - Applied Genomics, Computation & Translational Core
CEDARS-SINAI, Beverly Hills, California, United States, 90211
Overview
The Genomics Core Biomedical Specialist
is responsible for wet lab procedures involving DNA/RNA isolation from cells, tissue, FFPE curls and scrapes, and blood. They possess expertise in the complete Next-Generation Sequencing (NGS) workflow, including DNA/RNA quantification and QC, manual library preparation, library quantification and QC, sequencing on the Illumina platform, and analysis of sequencing metrics. They ensure accurate and up-to-date electronic documentation using an electronic lab notebook, project management software, and laboratory information management system. They manually prepare libraries for bulk cell, single cell, and spatial omics, covering transcriptomics (RNA-Seq), genomics (WGS/WES), epigenomics (WGBS, ATAC-Seq), and metagenomics (16S/ITS amplicon sequencing, shotgun). Overall, this role involves hands-on work in a wet lab environment, focusing on DNA/RNA isolation and various aspects of the NGS workflow, as well as documentation and library preparation for different omics applications. Primary Duties and Responsibilities
Train, guide, and supervise a team of junior associates. Train and supervise junior associates on Next-Generation Sequencing experiments, procedures, and day-to-day service request fulfillment and lab operations. Effectively communicate needs and requirements to junior associates for management of service requests and day-to-day lab operations. Manage junior associate performance, mentor and coach, and seek opportunities to help them grow. Assign service requests to junior associates and ensure their proper execution and successful completion. Ensure the availability of all required resources for the execution of service requests. Liaise with investigators regarding service requests. Participate in the planning, execution, and troubleshooting of service requests. Provide research input on new technologies and assays for testing to possibly develop and implement new service offerings. Execute or manage the execution of projects testing new technologies, assays, or kits. Analyze, summarize, and present findings at monthly R&D meetings. Assist with the implementation of new technologies, assays, or kits into new service offerings by writing and reviewing standard operating procedures, service guidelines, and staff training guides, and training junior associates. Receive, store, and document samples. Attend meetings and seminars/webinars as assigned. Qualifications
Education: Bachelor of Sciences in Genetics, Genomics, Molecular Biology, or Biological Sciences is required. Master’s degree is preferred. Experience & Skills: Two (2) years of laboratory experience are required. Five (5) years is preferred. Minimum 3 years of experience performing NGS library preparation in bulk cell genomics, transcriptomics, and metagenomics. Minimum 2 years of experience in the operation of Illumina sequencers and Agilent Technologies fragment analyzers. Minimum 1 year of supervisory and training experience. Experience in single cell NGS assays and cell biology is preferred. Critical analysis, problem-solving, troubleshooting, multitasking, time management, attention to detail, and ability to work independently with minimal supervision. Professional interaction with teammates, researchers, investigators, and support staff of diverse backgrounds. Ability to adapt to rapidly changing priorities in a fast-paced laboratory environment. Self-motivated, takes initiative, enjoys learning, and willing to take on new challenges while executing complex procedures. Works cooperatively with others to achieve goals. Proficient in PC or Mac and Microsoft Word, Excel, and PowerPoint.
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The Genomics Core Biomedical Specialist
is responsible for wet lab procedures involving DNA/RNA isolation from cells, tissue, FFPE curls and scrapes, and blood. They possess expertise in the complete Next-Generation Sequencing (NGS) workflow, including DNA/RNA quantification and QC, manual library preparation, library quantification and QC, sequencing on the Illumina platform, and analysis of sequencing metrics. They ensure accurate and up-to-date electronic documentation using an electronic lab notebook, project management software, and laboratory information management system. They manually prepare libraries for bulk cell, single cell, and spatial omics, covering transcriptomics (RNA-Seq), genomics (WGS/WES), epigenomics (WGBS, ATAC-Seq), and metagenomics (16S/ITS amplicon sequencing, shotgun). Overall, this role involves hands-on work in a wet lab environment, focusing on DNA/RNA isolation and various aspects of the NGS workflow, as well as documentation and library preparation for different omics applications. Primary Duties and Responsibilities
Train, guide, and supervise a team of junior associates. Train and supervise junior associates on Next-Generation Sequencing experiments, procedures, and day-to-day service request fulfillment and lab operations. Effectively communicate needs and requirements to junior associates for management of service requests and day-to-day lab operations. Manage junior associate performance, mentor and coach, and seek opportunities to help them grow. Assign service requests to junior associates and ensure their proper execution and successful completion. Ensure the availability of all required resources for the execution of service requests. Liaise with investigators regarding service requests. Participate in the planning, execution, and troubleshooting of service requests. Provide research input on new technologies and assays for testing to possibly develop and implement new service offerings. Execute or manage the execution of projects testing new technologies, assays, or kits. Analyze, summarize, and present findings at monthly R&D meetings. Assist with the implementation of new technologies, assays, or kits into new service offerings by writing and reviewing standard operating procedures, service guidelines, and staff training guides, and training junior associates. Receive, store, and document samples. Attend meetings and seminars/webinars as assigned. Qualifications
Education: Bachelor of Sciences in Genetics, Genomics, Molecular Biology, or Biological Sciences is required. Master’s degree is preferred. Experience & Skills: Two (2) years of laboratory experience are required. Five (5) years is preferred. Minimum 3 years of experience performing NGS library preparation in bulk cell genomics, transcriptomics, and metagenomics. Minimum 2 years of experience in the operation of Illumina sequencers and Agilent Technologies fragment analyzers. Minimum 1 year of supervisory and training experience. Experience in single cell NGS assays and cell biology is preferred. Critical analysis, problem-solving, troubleshooting, multitasking, time management, attention to detail, and ability to work independently with minimal supervision. Professional interaction with teammates, researchers, investigators, and support staff of diverse backgrounds. Ability to adapt to rapidly changing priorities in a fast-paced laboratory environment. Self-motivated, takes initiative, enjoys learning, and willing to take on new challenges while executing complex procedures. Works cooperatively with others to achieve goals. Proficient in PC or Mac and Microsoft Word, Excel, and PowerPoint.
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