Johnson Service Group
Johnson Service Group (JSG) is currently looking for a Scientistwho has knowledge of cell culture methods and sterile technique. This is a contract hire opportunity with one of the world’s premier research-intensive biopharmaceutical companies and is located in Cambridge MA. Pay: $44/hour Primary responsibilities include culturing of stable cell lines, primary and iPS-derived microglia to support the study of disease associated functions and phenotypes that will support identification and validation of novel therapeutic targets to treat neurodegenerative diseases. Education and Experience: BS/BA degree in Biology, Neuroscience, Molecular Biology Bioengineering, Biotechnology or equivalent degree with a at least 2+ year of industry or academia experience Required skills and competencies: Strong general knowledge of cell culture methods and sterile technique. Experience working immortalized cells lines, primary rodent neurons and glia (e.g. microglia). human iPS-derived CNS cell models including microglia and neurons. Understanding of standard cell transfection techniques using lipid-based and electroporation methodology Cell staining methods including Immunofluorescence and cell masks or cell structural staining methods. Some experience with method optimization, troubleshooting and strong critical thinking skills Proven experience in image acquisition and strong microscopy skills and strong working knowledge of mouse neuroanatomy Ability to work independently on data analysis including a practical knowledge of statistics. Preferred skills: Experience with plate based high content imaging microscopy live cell imaging Demonstrated experience with quantitative image analysis. •Working understanding of biochemical and molecular biology techniques including protein and RNA extraction, quantitation and detection methods such as western-blot, ELISA and qPCR Experience with cellular signaling and functional studies including phagocytosis and/or cell motility measures Johnson Service Group (JSG) is an Equal Opportunity Employer. JSG provides equal employment opportunities to all applicants and employees without regard to race, color, religion, sex, age, sexual orientation, gender identity, national origin, disability, marital status, protected veteran status, or any other characteristic protected by law. Allen Mudalel (610) 828-7356 amudalel@jsginc.com 515 Grove St. Suite 3G • Haddon Heights, NJ 08035 • jsginc.com