Takeda
Overview
Associate Scientist role at Takeda in Cambridge, MA. Base pay range
$99,500.00/yr - $156,420.00/yr Job Description
Pursuant to Section 20 C.F.R. 656.10, Takeda Development Center Americas, Inc. files for Alien Employment Certification for the following job opportunity. Responsibilities
Spearhead the development and deployment of high-throughput platforms to accelerate early discovery of engineered γδT cell therapies. Establish scalable workflows using automated liquid handling and high-content imaging platforms to streamline construct screening across primary donor sources. Implement robotic protocols for cell isolation, transduction, and expansion in 96 & 384 well formats to enable parallel testing of CAR variants with consistent throughput and reproducibility. Execute high-throughput screening of antibody discovery campaigns to identify lead candidates for CAR binder design and optimization. Perform high-throughput array-based pooled screening to evaluate novel CAR signaling domain constructs for functional activity. Design, execute, and optimize high-throughput in vitro assays for lead hit identification, including cytokine profiling, 2D/3D cytotoxicity, and phenotypic flow cytometry. Collaborate with automation engineers and data scientists to troubleshoot workflow issues, validate assay performance, and drive integration of outputs into global systems. Develop standardized SOPs, defined QC metrics, and contribute to global harmonization to support assay transferability across biologics and cell therapy pipelines. Mentor junior scientists, manage laboratory resources, and coordinate cross-functional project timelines within a matrixed team. Present key findings to cross-functional stakeholders to support early go/no-go decisions and alignment with pipeline strategy and IND-enabling milestones. Qualifications
Master’s in Biology, Biotechnology, or related field with 3 years of related experience OR Bachelor’s in Biology, Biotechnology, or related field with 5 years of related experience. Experience designing and executing experiments to assess anti-tumor functionality of allogeneic innate CAR cell therapies (e.g., cord blood derived CAR-NK, CAR-gdT), including analysis of cytokine and cytotoxicity assays and repeated antigen stimulation. Experience with cell isolation, activation, transduction, expansion, and cryopreservation of PBMC-derived CAR-T cells for functional characterization; automated or semi-automated liquid handling for in vitro assay setup. Experience with cord-blood derived NK cells and gamma retroviral vector production and transduction of innate immune cells; multiparameter flow cytometry (8+ color). Cell culture, immunofluorescence imaging, and plasmid cloning. Details
Location: Cambridge, MA Worker Type: Employee Worker Sub-Type: Regular Time Type: Full time Seniority level: Entry level Employment type: Full-time Job function: Research, Analyst, and Information Technology Industries: Pharmaceutical Manufacturing EOE. Qualified applicants can apply at https://jobs.takeda.com. Please reference job #R0163160.
#J-18808-Ljbffr
Associate Scientist role at Takeda in Cambridge, MA. Base pay range
$99,500.00/yr - $156,420.00/yr Job Description
Pursuant to Section 20 C.F.R. 656.10, Takeda Development Center Americas, Inc. files for Alien Employment Certification for the following job opportunity. Responsibilities
Spearhead the development and deployment of high-throughput platforms to accelerate early discovery of engineered γδT cell therapies. Establish scalable workflows using automated liquid handling and high-content imaging platforms to streamline construct screening across primary donor sources. Implement robotic protocols for cell isolation, transduction, and expansion in 96 & 384 well formats to enable parallel testing of CAR variants with consistent throughput and reproducibility. Execute high-throughput screening of antibody discovery campaigns to identify lead candidates for CAR binder design and optimization. Perform high-throughput array-based pooled screening to evaluate novel CAR signaling domain constructs for functional activity. Design, execute, and optimize high-throughput in vitro assays for lead hit identification, including cytokine profiling, 2D/3D cytotoxicity, and phenotypic flow cytometry. Collaborate with automation engineers and data scientists to troubleshoot workflow issues, validate assay performance, and drive integration of outputs into global systems. Develop standardized SOPs, defined QC metrics, and contribute to global harmonization to support assay transferability across biologics and cell therapy pipelines. Mentor junior scientists, manage laboratory resources, and coordinate cross-functional project timelines within a matrixed team. Present key findings to cross-functional stakeholders to support early go/no-go decisions and alignment with pipeline strategy and IND-enabling milestones. Qualifications
Master’s in Biology, Biotechnology, or related field with 3 years of related experience OR Bachelor’s in Biology, Biotechnology, or related field with 5 years of related experience. Experience designing and executing experiments to assess anti-tumor functionality of allogeneic innate CAR cell therapies (e.g., cord blood derived CAR-NK, CAR-gdT), including analysis of cytokine and cytotoxicity assays and repeated antigen stimulation. Experience with cell isolation, activation, transduction, expansion, and cryopreservation of PBMC-derived CAR-T cells for functional characterization; automated or semi-automated liquid handling for in vitro assay setup. Experience with cord-blood derived NK cells and gamma retroviral vector production and transduction of innate immune cells; multiparameter flow cytometry (8+ color). Cell culture, immunofluorescence imaging, and plasmid cloning. Details
Location: Cambridge, MA Worker Type: Employee Worker Sub-Type: Regular Time Type: Full time Seniority level: Entry level Employment type: Full-time Job function: Research, Analyst, and Information Technology Industries: Pharmaceutical Manufacturing EOE. Qualified applicants can apply at https://jobs.takeda.com. Please reference job #R0163160.
#J-18808-Ljbffr