St. Jude Children's Research Hospital
Associate Scientist in Center of Excellence for Structural Cell Biology
St. Jude Children's Research Hospital, Memphis, Tennessee, us, 37544
Associate Scientist in Center of Excellence for Structural Cell Biology
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Associate Scientist in Center of Excellence for Structural Cell Biology
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St. Jude Children's Research Hospital We are seeking a highly motivated and skilled Associate Scientist with expertise in cryo-Electron Tomography (cryo-ET) and/or cryo-Volume EM (cryo-vEM) to join our team. This position will focus on developing and optimizing sample preparation workflows for cells and tissue biopsies with a focus on vitrification via high-pressure freezing (HPF) and cryo-focused ion beam (cryo-FIB) milling. The successful candidate will also be involved in data acquisition, image processing, segmentation and workflow development to advance structural cell biology research. The World’s Brightest Minds Always Innovate At St. Jude Children’s Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That’s why we provide world-class facilities. State-of-the-art technologies. Extraordinary support. And a collaborative, bench-to-bedside environment where you can see, firsthand, how your science translates into survival for the children we serve. Key Responsibilities: Develop and optimize sample preparation workflows for cryo-ET and cryo-vEM, including cell growth on EM grids, tissue sectioning, vitrification, and cryo-FIB milling on gird or with lift-out. Operate cryo-FIBSEM systems (Aquilos, Arctis or Hydra) with integrated fluorescence for lamella preparation and volume imaging. Perform cryo-correlative microscopy workflows and cryo-light microscopy as needed. Support tilt-series data collection using SerielEM or Tomography 5. Process and analyze cryo-ET or volume-EM datasets using tools such as ImageJ, Napari, Amira or Dragonfly. Contribute to workflow automation and hardware/software innovation in collaboration with internal teams and industry partners. Provide training and support to researchers on sample prep, data collection, and image processing techniques. Assist with instrument maintenance and protocol development to support high-throughput imaging pipelines. Minimum Education and/or Training: Bachelor's degree in relevant scientific area is required. Minimum Experience: Seven (7) years of relevant work experience is required with a Bachelor’s degree. Five (5) years of relevant work experience is required with a Master's degree. Two (2) years of relevant work experience is required with a PhD. Preferred Qualifications & Experience: PhD or equivalent degree in Structural Biology, Biophysics, Biochemistry, Molecular Biology, or a related field. Hands-on experience in cryo-ET and/or cryo-vEM workflows, including sample vitrification, HPF and cryo-FIB milling. Proficiency in operation of FIB-SEM microscopes. Experience in cryo-ET data collection using Titan Krios, Glacios, or Arctica microscopes. Familiarity with scripting or programming (e.g., Python, MATLAB) is advantageous. Experience with cryo-correlative workflows and multimodal imaging is a plus. Strong problem-solving skills. Ability to work independently and collaborate within an interdisciplinary team. Benefits Your health and well-being are important to us. That's why St. Jude Children's Research Hospital provides employees with high-quality benefits choices. St. Jude is an Equal Opportunity Employer
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Join to apply for the
Associate Scientist in Center of Excellence for Structural Cell Biology
role at
St. Jude Children's Research Hospital We are seeking a highly motivated and skilled Associate Scientist with expertise in cryo-Electron Tomography (cryo-ET) and/or cryo-Volume EM (cryo-vEM) to join our team. This position will focus on developing and optimizing sample preparation workflows for cells and tissue biopsies with a focus on vitrification via high-pressure freezing (HPF) and cryo-focused ion beam (cryo-FIB) milling. The successful candidate will also be involved in data acquisition, image processing, segmentation and workflow development to advance structural cell biology research. The World’s Brightest Minds Always Innovate At St. Jude Children’s Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That’s why we provide world-class facilities. State-of-the-art technologies. Extraordinary support. And a collaborative, bench-to-bedside environment where you can see, firsthand, how your science translates into survival for the children we serve. Key Responsibilities: Develop and optimize sample preparation workflows for cryo-ET and cryo-vEM, including cell growth on EM grids, tissue sectioning, vitrification, and cryo-FIB milling on gird or with lift-out. Operate cryo-FIBSEM systems (Aquilos, Arctis or Hydra) with integrated fluorescence for lamella preparation and volume imaging. Perform cryo-correlative microscopy workflows and cryo-light microscopy as needed. Support tilt-series data collection using SerielEM or Tomography 5. Process and analyze cryo-ET or volume-EM datasets using tools such as ImageJ, Napari, Amira or Dragonfly. Contribute to workflow automation and hardware/software innovation in collaboration with internal teams and industry partners. Provide training and support to researchers on sample prep, data collection, and image processing techniques. Assist with instrument maintenance and protocol development to support high-throughput imaging pipelines. Minimum Education and/or Training: Bachelor's degree in relevant scientific area is required. Minimum Experience: Seven (7) years of relevant work experience is required with a Bachelor’s degree. Five (5) years of relevant work experience is required with a Master's degree. Two (2) years of relevant work experience is required with a PhD. Preferred Qualifications & Experience: PhD or equivalent degree in Structural Biology, Biophysics, Biochemistry, Molecular Biology, or a related field. Hands-on experience in cryo-ET and/or cryo-vEM workflows, including sample vitrification, HPF and cryo-FIB milling. Proficiency in operation of FIB-SEM microscopes. Experience in cryo-ET data collection using Titan Krios, Glacios, or Arctica microscopes. Familiarity with scripting or programming (e.g., Python, MATLAB) is advantageous. Experience with cryo-correlative workflows and multimodal imaging is a plus. Strong problem-solving skills. Ability to work independently and collaborate within an interdisciplinary team. Benefits Your health and well-being are important to us. That's why St. Jude Children's Research Hospital provides employees with high-quality benefits choices. St. Jude is an Equal Opportunity Employer
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