Washington University in St. Louis
Staff Scientist (Computational – Genomics) - Genetics
Washington University in St. Louis, Saint Louis, Missouri, United States, 63146
Staff Scientist (Computational – Genomics) - Genetics
Position Summary
The Cremins lab works at the spatial biology-technology interface to understand chromatin-to-synapse communication during neural circuit activation in the mammalian brain. We aim to understand how chromatin works through long-range physical folding mechanisms to encode neuronal specification and long-term synaptic plasticity in healthy and diseased neural circuits. We pursue a multi-disciplinary approach integrating data across biological scales in the brain, including molecular Chromosome-Conformation-Capture sequencing technologies, single-cell imaging, optogenetics, genome engineering, induced pluripotent stem cell differentiation to neurons/organoids, and in vitro and in vivo electrophysiological measurements. Our long-term scientific goal is to dissect the fundamental mechanisms by which chromatin architecture causally governs genome function and, ultimately, long-term synaptic plasticity and neural circuit features in healthy mammalian brains as well as during the onset and progression of neurodegenerative and neurodevelopmental disease states. Our long-term mentorship goal is to develop a diverse cohort of next-generation scientists cross-trained in molecular and computational approaches. We seek to create a positive, high-energy environment with open and honest communication to empower individuals to discover and refine their purpose and grow into the best versions of themselves.
Position Summary and Responsibilities
The candidate will organize, manage, and curate big data on the cluster and find biological patterns in a wide range of genetic and epigenetic sequencing and imaging data to facilitate the paper figures of lab members. Candidate will train on the lab’s fundamental algorithms and run them in a collaborative manner with other team members to generate paper figures and make discoveries. Collaborative code building for team member papers will involve mastery and application of existing algorithms and tools for understanding and detecting TADs, subTADs, loops, and compartments in Hi-C and MicroC data and integrating the 3D genome features with underlying chromatin marks. As the candidate advances in training, they will develop new algorithms to find TADs and loops in single cell genomics data, as well as to analyze in situ DNA FISH and merFISH data.
This role is ideal for you if you have a strong interest and motivation toward publishing results and will contribute to the preparation of multiple manuscripts, including making figures and writing methods and results. This role is ideal for you if you enjoy the excitement of a bleeding-edge scientific laboratory and welcome the challenge of a fast-paced environment and working collaboratively according to a positive, warm, and community-focused scientific culture. Ideal for post-baccalaureate students eager to do PhD level research.
Primary Duties & Responsibilities:
Following instructions and discussions with the principal investigator, designs research protocols, including developing procedures for the collection, verification and management of data.
Performs computational tasks under direction and collaboration with other graduate students, postdocs, and scientists in the laboratory (given concepts/ ideas, and/ or data from individual lab members, creates algorithms to analyze data generated by their projects to create paper figures and make discoveries).
Trains to achieve mastery and excellence in code to call compartments, TADs, subTADs, loops in Hi-C/Micro-C data, as well as in peak calling of Cut&Run and Cut&Tag data.
In collaboration with lab members, writes methods sections and figures for research papers where the analysis is used in the manuscripts.
Performs complex statistical analysis of data collected and writes interpretative reports. Verifies the correctness of the data submitted and makes recommendations based on these analyses.
Documents research topics and prepares and submits papers based on research work to publications and committees for publication or presentation to peers.
Organizes the lab cluster according to lab protocols and standards every week.
Leads the movement of data from base space to the cluster and the keeping of careful records for sequencing runs and sample names for each project.
Runs established codes for read mapping and peak calling for lab’s foundational data across projects.
Performs other duties as assigned.
Working Conditions:
Works in a laboratory environment with potential exposure to biological and chemical hazards.
Must be physically able to wear protective equipment and to provide standard care to research animals.
Education:
Required Qualifications
Master’s degree or combination of education and/or experience may substitute for minimum education.
Certifications:
No specific certification is required for this position.
Work Experience:
Laboratory Operations (3 Years)
Skills:
Not Applicable
Driver's License:
A driver's license is not required for this position.
Preferred Qualifications:
Ph.D. - Doctor of Philosophy
More About This Job
Exceptional commitment to upholding the collaborative ethos of the Cremins laboratory.
Strong skills in verbal and written communication with a dedication to open transparent communication with mentors and peers.
Demonstrated ability to handle daily laboratory challenges with a dedication to the values of reconciliation, unity, mutual honor, and respect.
Benefits Statement
Personal: Up to 22 days of vacation, 10 recognized holidays, and sick time.
Health: Competitive health insurance packages with priority appointments and lower copays/coinsurance.
Transit: Free Metro transit U-Pass for eligible employees.
Retirement: WashU 403(b) plan with employer contributions starting at 7%.
EEO Statement
Washington University in St. Louis is committed to the principles and practices of equal employment opportunity and especially encourages applications by those from underrepresented groups. It is the University’s policy to provide equal opportunity and access to persons in all job titles without regard to race, ethnicity, color, national origin, age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. Washington University is dedicated to building a community of individuals who are committed to contributing to an inclusive environment – fostering respect for all and welcoming individuals from diverse backgrounds, experiences and perspectives. Individuals with a commitment to these values are encouraged to apply.
#J-18808-Ljbffr
The Cremins lab works at the spatial biology-technology interface to understand chromatin-to-synapse communication during neural circuit activation in the mammalian brain. We aim to understand how chromatin works through long-range physical folding mechanisms to encode neuronal specification and long-term synaptic plasticity in healthy and diseased neural circuits. We pursue a multi-disciplinary approach integrating data across biological scales in the brain, including molecular Chromosome-Conformation-Capture sequencing technologies, single-cell imaging, optogenetics, genome engineering, induced pluripotent stem cell differentiation to neurons/organoids, and in vitro and in vivo electrophysiological measurements. Our long-term scientific goal is to dissect the fundamental mechanisms by which chromatin architecture causally governs genome function and, ultimately, long-term synaptic plasticity and neural circuit features in healthy mammalian brains as well as during the onset and progression of neurodegenerative and neurodevelopmental disease states. Our long-term mentorship goal is to develop a diverse cohort of next-generation scientists cross-trained in molecular and computational approaches. We seek to create a positive, high-energy environment with open and honest communication to empower individuals to discover and refine their purpose and grow into the best versions of themselves.
Position Summary and Responsibilities
The candidate will organize, manage, and curate big data on the cluster and find biological patterns in a wide range of genetic and epigenetic sequencing and imaging data to facilitate the paper figures of lab members. Candidate will train on the lab’s fundamental algorithms and run them in a collaborative manner with other team members to generate paper figures and make discoveries. Collaborative code building for team member papers will involve mastery and application of existing algorithms and tools for understanding and detecting TADs, subTADs, loops, and compartments in Hi-C and MicroC data and integrating the 3D genome features with underlying chromatin marks. As the candidate advances in training, they will develop new algorithms to find TADs and loops in single cell genomics data, as well as to analyze in situ DNA FISH and merFISH data.
This role is ideal for you if you have a strong interest and motivation toward publishing results and will contribute to the preparation of multiple manuscripts, including making figures and writing methods and results. This role is ideal for you if you enjoy the excitement of a bleeding-edge scientific laboratory and welcome the challenge of a fast-paced environment and working collaboratively according to a positive, warm, and community-focused scientific culture. Ideal for post-baccalaureate students eager to do PhD level research.
Primary Duties & Responsibilities:
Following instructions and discussions with the principal investigator, designs research protocols, including developing procedures for the collection, verification and management of data.
Performs computational tasks under direction and collaboration with other graduate students, postdocs, and scientists in the laboratory (given concepts/ ideas, and/ or data from individual lab members, creates algorithms to analyze data generated by their projects to create paper figures and make discoveries).
Trains to achieve mastery and excellence in code to call compartments, TADs, subTADs, loops in Hi-C/Micro-C data, as well as in peak calling of Cut&Run and Cut&Tag data.
In collaboration with lab members, writes methods sections and figures for research papers where the analysis is used in the manuscripts.
Performs complex statistical analysis of data collected and writes interpretative reports. Verifies the correctness of the data submitted and makes recommendations based on these analyses.
Documents research topics and prepares and submits papers based on research work to publications and committees for publication or presentation to peers.
Organizes the lab cluster according to lab protocols and standards every week.
Leads the movement of data from base space to the cluster and the keeping of careful records for sequencing runs and sample names for each project.
Runs established codes for read mapping and peak calling for lab’s foundational data across projects.
Performs other duties as assigned.
Working Conditions:
Works in a laboratory environment with potential exposure to biological and chemical hazards.
Must be physically able to wear protective equipment and to provide standard care to research animals.
Education:
Required Qualifications
Master’s degree or combination of education and/or experience may substitute for minimum education.
Certifications:
No specific certification is required for this position.
Work Experience:
Laboratory Operations (3 Years)
Skills:
Not Applicable
Driver's License:
A driver's license is not required for this position.
Preferred Qualifications:
Ph.D. - Doctor of Philosophy
More About This Job
Exceptional commitment to upholding the collaborative ethos of the Cremins laboratory.
Strong skills in verbal and written communication with a dedication to open transparent communication with mentors and peers.
Demonstrated ability to handle daily laboratory challenges with a dedication to the values of reconciliation, unity, mutual honor, and respect.
Benefits Statement
Personal: Up to 22 days of vacation, 10 recognized holidays, and sick time.
Health: Competitive health insurance packages with priority appointments and lower copays/coinsurance.
Transit: Free Metro transit U-Pass for eligible employees.
Retirement: WashU 403(b) plan with employer contributions starting at 7%.
EEO Statement
Washington University in St. Louis is committed to the principles and practices of equal employment opportunity and especially encourages applications by those from underrepresented groups. It is the University’s policy to provide equal opportunity and access to persons in all job titles without regard to race, ethnicity, color, national origin, age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. Washington University is dedicated to building a community of individuals who are committed to contributing to an inclusive environment – fostering respect for all and welcoming individuals from diverse backgrounds, experiences and perspectives. Individuals with a commitment to these values are encouraged to apply.
#J-18808-Ljbffr