Net2Source (N2S)
Biologist (3-5 years) Biologist (3-5 years)
Net2Source (N2S), Boston, Massachusetts, us, 02298
Overview
Biologist (3-5 years). Duration: 18 months. Location: Boston, MA 02210. Pay: $50.75/hr on W2. Title: Biologist. Client: Net2Source is seeking a Sr. Scientist in Immunology to support the development of allogeneic cell therapies by developing immune cell-based assays, executing in vitro experiments, and assessing immune responses to different gene-edited cells. You will contribute to the early-stage validation of gene edits and work with cross-functional teams to advance cell therapy programs to preclinical development. Responsibilities
Develop and optimize cell-based assays using primary human immune cells, such as mixed lymphocyte reaction, T-cell and NK-cell cytotoxicity assays, phagocytosis assays, etc. Isolate and culture different immune cells from PBMCs or fresh whole blood using immunomagnetic isolation techniques Differentiate monocytes to macrophages or dendritic cells as needed Assess immune checkpoint receptor expression using multi-parameter flow cytometry Utilize gene editing techniques, including CRISPR to create knockouts or transgenic cell lines Gene-edit different cell types including iPSCs, primary cells, and immortalized cell lines Use methods such as cell sorting and single-cell cloning to create gene-edited clonal cell lines Design and execute experiments and analyze data to evaluate the efficacy of gene-edited cells in in vitro assays Communicate experimental data effectively through written reports and oral presentations Demonstrate flexibility and motivation to learn and apply new technologies for biological assays Qualifications
Bachelor's degree in Immunology, Cell Biology, or related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in immunology Master's degree in Immunology, Cell Biology, or related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in immunology Additional Skills/Preferences
Experience with live cell analysis platforms (e.g., Incucyte, Xcelligence) is a plus Proficient in developing in vitro assays with human cells (PBMCs, whole blood) Experience with immune cell and cytokine analytical techniques (multiplex ELISA, MSD, flow cytometry) Prior work with CRISPR/Cas9 gene editing Experience with cell culture of primary and mammalian cell lines Strong analytical, problem-solving, and decision-making skills Excellent interpersonal and communication skills Ability to work independently and as part of a team Why Work With Us?
We believe in more than just jobs—we build careers. Net2Source champions leadership at all levels, celebrates diverse perspectives, and empowers you to make an impact. Expect work-life balance, professional growth, and a collaborative culture where your ideas matter. Our Commitment to Inclusion & Equity
Net2Source is an equal opportunity employer, dedicated to fostering a workplace where diverse talents and perspectives are valued. We make all employment decisions based on merit, ensuring a culture of respect, fairness, and opportunity for all, regardless of age, gender, ethnicity, disability, or other protected characteristics. Awards & Recognition
America’s Most Honored Businesses (Top 10%), Fastest-Growing Staffing Firm by Staffing Industry Analysts, INC 5000 List for Eight Consecutive Years, Top 100 by Dallas Business Journal, Spirit of Alliance Award by Agile1. Ready to Level Up Your Career? Click Apply Now and let’s make it happen.
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Biologist (3-5 years). Duration: 18 months. Location: Boston, MA 02210. Pay: $50.75/hr on W2. Title: Biologist. Client: Net2Source is seeking a Sr. Scientist in Immunology to support the development of allogeneic cell therapies by developing immune cell-based assays, executing in vitro experiments, and assessing immune responses to different gene-edited cells. You will contribute to the early-stage validation of gene edits and work with cross-functional teams to advance cell therapy programs to preclinical development. Responsibilities
Develop and optimize cell-based assays using primary human immune cells, such as mixed lymphocyte reaction, T-cell and NK-cell cytotoxicity assays, phagocytosis assays, etc. Isolate and culture different immune cells from PBMCs or fresh whole blood using immunomagnetic isolation techniques Differentiate monocytes to macrophages or dendritic cells as needed Assess immune checkpoint receptor expression using multi-parameter flow cytometry Utilize gene editing techniques, including CRISPR to create knockouts or transgenic cell lines Gene-edit different cell types including iPSCs, primary cells, and immortalized cell lines Use methods such as cell sorting and single-cell cloning to create gene-edited clonal cell lines Design and execute experiments and analyze data to evaluate the efficacy of gene-edited cells in in vitro assays Communicate experimental data effectively through written reports and oral presentations Demonstrate flexibility and motivation to learn and apply new technologies for biological assays Qualifications
Bachelor's degree in Immunology, Cell Biology, or related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in immunology Master's degree in Immunology, Cell Biology, or related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in immunology Additional Skills/Preferences
Experience with live cell analysis platforms (e.g., Incucyte, Xcelligence) is a plus Proficient in developing in vitro assays with human cells (PBMCs, whole blood) Experience with immune cell and cytokine analytical techniques (multiplex ELISA, MSD, flow cytometry) Prior work with CRISPR/Cas9 gene editing Experience with cell culture of primary and mammalian cell lines Strong analytical, problem-solving, and decision-making skills Excellent interpersonal and communication skills Ability to work independently and as part of a team Why Work With Us?
We believe in more than just jobs—we build careers. Net2Source champions leadership at all levels, celebrates diverse perspectives, and empowers you to make an impact. Expect work-life balance, professional growth, and a collaborative culture where your ideas matter. Our Commitment to Inclusion & Equity
Net2Source is an equal opportunity employer, dedicated to fostering a workplace where diverse talents and perspectives are valued. We make all employment decisions based on merit, ensuring a culture of respect, fairness, and opportunity for all, regardless of age, gender, ethnicity, disability, or other protected characteristics. Awards & Recognition
America’s Most Honored Businesses (Top 10%), Fastest-Growing Staffing Firm by Staffing Industry Analysts, INC 5000 List for Eight Consecutive Years, Top 100 by Dallas Business Journal, Spirit of Alliance Award by Agile1. Ready to Level Up Your Career? Click Apply Now and let’s make it happen.
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