Kcas, Llc
Overview
KCAS Bio is a recognized leader in global spectral flow cytometry and conventional flow cytometry and cell sorting services to support non-clinical and clinical studies. With validated methods, flow cytometry enables powerful biomarker screening and multi-parameter cellular profiling. Validated Assays
Established protocols for biomarker discovery and immunogenicity assays that ensure reproducible results across preclinical and clinical studies, giving sponsors confidence in data quality while accelerating decision-making. Fast Turnaround
Streamlined workflows for rapid sample processing and analysis to keep studies on schedule. Regulatory Expertise
GxP-compliant services fully aligned with FDA and EMA guidelines to support navigation through regulatory requirements. State-of-the-Art Technology
High-dimensional cellular profiling and precise cell sorting using platforms including Cytek Aurora, BD Fortessa, and BD FACSymphony to provide deeper insights and robust, actionable data. What is flow cytometry?
Flow cytometry is a laser-based technique that uses a fluidics-based system and fluorochrome-labeled samples to detect and measure the physical and physiological characteristics of a population of cells or particles. Tens of thousands of live cells or particles can be examined and analyzed for multiple parameters in a single test sample. Applications include PK/PD assessments, cellular phenotyping and functional assays, biomarker detection, proliferation, and toxicology. We can tailor a flow cytometry method to your research needs. Advance your program with our global flow cytometry expertise. Brian Wile, Ph.D. and Adam Cotty, Ph.D. from the KCAS Bio – Philadelphia site share insights about our labs, scientists, projects and use-cases for flow cytometry. “We are expanding investigators’ ability to explore the immune system and how it reacts to disease indications and new drugs during drug development.” Applications of flow cytometry in drug development
Oncology:
Immune profiling (T cells, NK cells, myeloid subsets), monitoring response to checkpoint inhibitors and CAR-T therapies, minimal residual disease detection. Infectious Diseases:
Immune monitoring, vaccine response assessment (B and T cells), and pathogen-host interaction studies. Autoimmune & Inflammatory Diseases:
Characterization of dysregulated immune subsets, biomarker discovery for diseases like rheumatoid arthritis, lupus, IBD, MS, and monitoring treatment efficacy in immunomodulatory therapies. Metabolic & Cardiovascular:
Role of immune cells in atherosclerosis, obesity, diabetes and biomarkers of inflammation and vascular damage. Discovery:
Exploratory flow panels to assess target engagement, immune cell characterization, biomarker discovery, functional screening assays and cytotoxicity compound screening. Preclinical Research:
GLP-validated flow cytometry assays to support toxicology, PK/PD, and immunophenotyping studies. Clinical Sample Analysis:
Central lab enabled flow services including validated panels, immunogenicity assessment, and longitudinal biomarker monitoring across global trials. Capabilities and assays for flow cytometry
KCAS Bio offers high-quality flow cytometry services tailored to deliver precise, reproducible insights. With optimized protocols and expert analysis, we help you characterize complex cell populations and generate reliable data that enables decision making. Immunophenotyping
Immunophenotyping by flow cytometry analyzes cell surface and intracellular markers to characterize cells within a heterogeneous population. We offer pre-validated panels, custom method development, and fit-for-purpose qualification and validation for studies across pre-clinical and clinical stages. Method Development and Qualification
At KCAS Bio, method development begins with the assay’s research application, guiding panel design, gating strategies, and sample preparation while ensuring GLP and GCLP compliance. Assays undergo fit-for-purpose qualification or validation following CLSI H62 guidelines to confirm robustness and reliability for pre-clinical and clinical studies. PBMC Isolation and Cryopreservation
PBMC isolation separates lymphocytes and monocytes from whole blood. High-quality methods preserve cell viability and function for reliable downstream analysis. We have trained staff, established SOPs, and global locations (US, Europe, APAC) to process clinical samples with precision and care. Cell Sorting
Fluorescence-activated cell sorting (FACS) enables physical separation of single or bulk cells from a heterogenous population. We optimize methods and staining panels to ensure successful sorting for various target populations and downstream applications. Magnetic Bead-Based Enrichment
Standard protocols for magnetic bead separation allow positive or negative selection for a variety of cell populations, enabling downstream applications including activation and expansion in culture. Globally Harmonized Sample Processing and Analysis
KCAS Bio has expanded spectral flow cytometry capabilities with Cytek Aurora 5-laser instruments deployed across multiple sites. Instruments are factory-harmonized to ensure consistent results across locations. Supported by a global quality system and multi-site training, we maintain aligned procedures worldwide for reliable data in global trials. Data Analysis
We offer customized data analysis workflows and deliverables with FDA, EMA, and regulatory-compliant reporting. Talk to us about custom packages and the advantages KCAS Bio can offer for your data analysis needs. Tailored Functional Assays
We develop and optimize functional assays that deliver precise, high-quality, regulatory-compliant data to support complex immunology programs. Examples include ICS, apoptosis, cell proliferation, phospho-flow, ex vivo stimulation and suppression assays. Click to learn about all functional assays including ICS, apoptosis, cell proliferation, phospho-flow, ex vivo stimulation and suppression assays. Technology and instrumentation for flow cytometry
Our laboratories are equipped with a broad range of flow cytometers optimized for analytical and cell-sorting applications, including high-throughput systems and flexible benchtop analyzers. Our conventional and spectral instruments are globally harmonized for consistency across processing sites. The 5-laser Cytek Aurora identifies up to 40 markers in a single experiment for expansive panels or autofluorescence reduction. BD LSRFortessa X-20 and BD LSR Fortessa platforms offer high signal resolution and multi-color capability with global harmonization across sites. BD FACSymphony S6 Cell Sorter is deployed to enable advanced sorting capabilities. Why work with KCAS Bio?
Full-Service Bioanalysis
From method development through final reporting for GLP/GCP-compliant flow cytometry, with expert teams based in the US, Europe and APAC region. Regulatory Expertise
Comprehensive, compliant data reporting that supports global regulatory submissions. Cutting-Edge Technology
Best-in-class conventional and spectral flow cytometers, cell sorters and analyzers. Flexible, Customized Workflows
Custom immunophenotyping, data analysis, PBMC isolation, and enrichment protocols to meet complex research needs. Broad Sample Type and Matrix Expertise
Plasma, serum, urine, CSF, and tissues plus unstable/rare matrices. Global Presence with Harmonized Quality
Consistent technical standards and harmonized operations worldwide in the US, Europe and APAC region. Tell us how we can help with your project
We’ve earned our reputation for delivering reliable, error-free data. We understand the importance of speed, flexibility, and consistency and only make promises we can keep.
#J-18808-Ljbffr
KCAS Bio is a recognized leader in global spectral flow cytometry and conventional flow cytometry and cell sorting services to support non-clinical and clinical studies. With validated methods, flow cytometry enables powerful biomarker screening and multi-parameter cellular profiling. Validated Assays
Established protocols for biomarker discovery and immunogenicity assays that ensure reproducible results across preclinical and clinical studies, giving sponsors confidence in data quality while accelerating decision-making. Fast Turnaround
Streamlined workflows for rapid sample processing and analysis to keep studies on schedule. Regulatory Expertise
GxP-compliant services fully aligned with FDA and EMA guidelines to support navigation through regulatory requirements. State-of-the-Art Technology
High-dimensional cellular profiling and precise cell sorting using platforms including Cytek Aurora, BD Fortessa, and BD FACSymphony to provide deeper insights and robust, actionable data. What is flow cytometry?
Flow cytometry is a laser-based technique that uses a fluidics-based system and fluorochrome-labeled samples to detect and measure the physical and physiological characteristics of a population of cells or particles. Tens of thousands of live cells or particles can be examined and analyzed for multiple parameters in a single test sample. Applications include PK/PD assessments, cellular phenotyping and functional assays, biomarker detection, proliferation, and toxicology. We can tailor a flow cytometry method to your research needs. Advance your program with our global flow cytometry expertise. Brian Wile, Ph.D. and Adam Cotty, Ph.D. from the KCAS Bio – Philadelphia site share insights about our labs, scientists, projects and use-cases for flow cytometry. “We are expanding investigators’ ability to explore the immune system and how it reacts to disease indications and new drugs during drug development.” Applications of flow cytometry in drug development
Oncology:
Immune profiling (T cells, NK cells, myeloid subsets), monitoring response to checkpoint inhibitors and CAR-T therapies, minimal residual disease detection. Infectious Diseases:
Immune monitoring, vaccine response assessment (B and T cells), and pathogen-host interaction studies. Autoimmune & Inflammatory Diseases:
Characterization of dysregulated immune subsets, biomarker discovery for diseases like rheumatoid arthritis, lupus, IBD, MS, and monitoring treatment efficacy in immunomodulatory therapies. Metabolic & Cardiovascular:
Role of immune cells in atherosclerosis, obesity, diabetes and biomarkers of inflammation and vascular damage. Discovery:
Exploratory flow panels to assess target engagement, immune cell characterization, biomarker discovery, functional screening assays and cytotoxicity compound screening. Preclinical Research:
GLP-validated flow cytometry assays to support toxicology, PK/PD, and immunophenotyping studies. Clinical Sample Analysis:
Central lab enabled flow services including validated panels, immunogenicity assessment, and longitudinal biomarker monitoring across global trials. Capabilities and assays for flow cytometry
KCAS Bio offers high-quality flow cytometry services tailored to deliver precise, reproducible insights. With optimized protocols and expert analysis, we help you characterize complex cell populations and generate reliable data that enables decision making. Immunophenotyping
Immunophenotyping by flow cytometry analyzes cell surface and intracellular markers to characterize cells within a heterogeneous population. We offer pre-validated panels, custom method development, and fit-for-purpose qualification and validation for studies across pre-clinical and clinical stages. Method Development and Qualification
At KCAS Bio, method development begins with the assay’s research application, guiding panel design, gating strategies, and sample preparation while ensuring GLP and GCLP compliance. Assays undergo fit-for-purpose qualification or validation following CLSI H62 guidelines to confirm robustness and reliability for pre-clinical and clinical studies. PBMC Isolation and Cryopreservation
PBMC isolation separates lymphocytes and monocytes from whole blood. High-quality methods preserve cell viability and function for reliable downstream analysis. We have trained staff, established SOPs, and global locations (US, Europe, APAC) to process clinical samples with precision and care. Cell Sorting
Fluorescence-activated cell sorting (FACS) enables physical separation of single or bulk cells from a heterogenous population. We optimize methods and staining panels to ensure successful sorting for various target populations and downstream applications. Magnetic Bead-Based Enrichment
Standard protocols for magnetic bead separation allow positive or negative selection for a variety of cell populations, enabling downstream applications including activation and expansion in culture. Globally Harmonized Sample Processing and Analysis
KCAS Bio has expanded spectral flow cytometry capabilities with Cytek Aurora 5-laser instruments deployed across multiple sites. Instruments are factory-harmonized to ensure consistent results across locations. Supported by a global quality system and multi-site training, we maintain aligned procedures worldwide for reliable data in global trials. Data Analysis
We offer customized data analysis workflows and deliverables with FDA, EMA, and regulatory-compliant reporting. Talk to us about custom packages and the advantages KCAS Bio can offer for your data analysis needs. Tailored Functional Assays
We develop and optimize functional assays that deliver precise, high-quality, regulatory-compliant data to support complex immunology programs. Examples include ICS, apoptosis, cell proliferation, phospho-flow, ex vivo stimulation and suppression assays. Click to learn about all functional assays including ICS, apoptosis, cell proliferation, phospho-flow, ex vivo stimulation and suppression assays. Technology and instrumentation for flow cytometry
Our laboratories are equipped with a broad range of flow cytometers optimized for analytical and cell-sorting applications, including high-throughput systems and flexible benchtop analyzers. Our conventional and spectral instruments are globally harmonized for consistency across processing sites. The 5-laser Cytek Aurora identifies up to 40 markers in a single experiment for expansive panels or autofluorescence reduction. BD LSRFortessa X-20 and BD LSR Fortessa platforms offer high signal resolution and multi-color capability with global harmonization across sites. BD FACSymphony S6 Cell Sorter is deployed to enable advanced sorting capabilities. Why work with KCAS Bio?
Full-Service Bioanalysis
From method development through final reporting for GLP/GCP-compliant flow cytometry, with expert teams based in the US, Europe and APAC region. Regulatory Expertise
Comprehensive, compliant data reporting that supports global regulatory submissions. Cutting-Edge Technology
Best-in-class conventional and spectral flow cytometers, cell sorters and analyzers. Flexible, Customized Workflows
Custom immunophenotyping, data analysis, PBMC isolation, and enrichment protocols to meet complex research needs. Broad Sample Type and Matrix Expertise
Plasma, serum, urine, CSF, and tissues plus unstable/rare matrices. Global Presence with Harmonized Quality
Consistent technical standards and harmonized operations worldwide in the US, Europe and APAC region. Tell us how we can help with your project
We’ve earned our reputation for delivering reliable, error-free data. We understand the importance of speed, flexibility, and consistency and only make promises we can keep.
#J-18808-Ljbffr