Allen Institute
Scientist III – Immunologist, iPSC-derived allogeneic T-cell engineering
Allen Institute, Seattle, Washington, us, 98127
Scientist III – Immunologist, iPSC-derived allogeneic T-cell engineering
Join Allen Institute as Scientist III – Immunologist, iPSC‑derived allogeneic T‑cell engineering. Allen Institute’s mission is to unlock the complexities of bioscience and advance human health through open science, multi‑scale, team‑oriented research.
Essential Functions
Play a founding role in creating an incubator designed to turn discoveries into cures
Shape and lead a cutting‑edge allogeneic (off‑the‑shelf) CAR‑T cell therapy program
Drive innovation at the intersection of iPSCs, CRISPR, and translational science
Develop robust protocols for deriving T‑cell lines from iPSCs and for applying state‑of‑the‑art CRISPR/Cas9 gene editing technologies to enhance their therapeutic properties
Design, execute, and analyze gene editing experiments using CRISPR/Cas9 and other technologies to modify iPSC‑derived T‑cells
Maintain and expand high‑quality human iPSC lines in a cGMP‑compliant manner
Engineer T‑cells to express chimeric antigen receptors (CARs) for targeted cancer therapy
Apply genome editing to remove or insert genes to enhance T‑cell potency, reduce T‑cell exhaustion, or mitigate off‑target toxicity (e.g., knocking out TCR to prevent Graft‑vs‑Host Disease)
Use multi‑color flow cytometry, cell sorting, and functional assays to characterize iPSC‑derived and engineered T‑cells
Assess T‑cell functionality, such as proliferation, cytokine production, and antigen‑specific cytotoxicity
Conduct molecular characterization using techniques like ddPCR, qPCR, Sanger sequencing, and next‑generation sequencing to confirm successful gene editing
Work effectively in a cross‑functional team, interacting with process development, analytical development, and regulatory affairs
Document all experimental procedures and results in electronic lab notebooks and technical reports
Present data and findings at internal meetings to advance CAR‑T pipeline programs
Required Experience and Education
Ph.D. in Immunology, Molecular Biology, Cell Biology, Bioengineering, or a related field; or equivalent combination of education and experience
5 years of relevant postdoctoral or industry experience or equivalent experience
Preferred Education and Experience
5 – 8 years relevant post‑doctoral experience
Strong hands‑on experience with human iPSC culture, maintenance, and differentiation protocols
Extensive practical experience with CRISPR/Cas9 or other gene editing technologies in mammalian cells
Proficiency in primary human T‑cell culture and engineering is a strong plus
Expertise in multi‑color flow cytometry and cell sorting for cell characterization
Experience with viral (lentivirus, AAV) and non‑viral gene delivery methods
Excellent communication and interpersonal skills
Ability to work independently and as part of a collaborative, fast‑paced team
Strong problem‑solving and troubleshooting abilities
Demonstrated team management
Demonstrated ability to build and lead a program
Physical Demands
Fine motor movements in fingers/hands to operate computers and other office equipment
Work Environment
Frequently required to sit, stand, walk, stoop, kneel or reach
Possible exposure and handling of laboratory animals
Laboratory atmosphere – possible exposure to chemical, biological or other hazardous substances
Position Type / Expected Hours of Work
This role is currently working onsite and is expected to work onsite for the majority of the working hours. We are a Washington State employer, and the primary work location for Allen Institute employees is 700 Dexter Ave N.; any remote work must be performed in Washington State.
Travel
The successful candidate may be invited to attend occasional national and international conferences
Additional Comments
Relocation assistance
Work visa sponsorship
Annualized Salary Range
$128,050 - $160,050 *
Final salary depends on required education for the role, experience, and level of skills relevant to the role, along with work location, where applicable
Benefits
Employees (and their families) are eligible to enroll in benefits per eligibility rules outlined in the Allen Institute’s Benefits Guide. These benefits include medical, dental, vision, and basic life insurance. Employees are also eligible to enroll in the Allen Institute’s 401k plan. Paid time off is also available as outlined in the Allen Institute’s Benefits Guide.
It is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodation for qualified individuals with disabilities.
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Essential Functions
Play a founding role in creating an incubator designed to turn discoveries into cures
Shape and lead a cutting‑edge allogeneic (off‑the‑shelf) CAR‑T cell therapy program
Drive innovation at the intersection of iPSCs, CRISPR, and translational science
Develop robust protocols for deriving T‑cell lines from iPSCs and for applying state‑of‑the‑art CRISPR/Cas9 gene editing technologies to enhance their therapeutic properties
Design, execute, and analyze gene editing experiments using CRISPR/Cas9 and other technologies to modify iPSC‑derived T‑cells
Maintain and expand high‑quality human iPSC lines in a cGMP‑compliant manner
Engineer T‑cells to express chimeric antigen receptors (CARs) for targeted cancer therapy
Apply genome editing to remove or insert genes to enhance T‑cell potency, reduce T‑cell exhaustion, or mitigate off‑target toxicity (e.g., knocking out TCR to prevent Graft‑vs‑Host Disease)
Use multi‑color flow cytometry, cell sorting, and functional assays to characterize iPSC‑derived and engineered T‑cells
Assess T‑cell functionality, such as proliferation, cytokine production, and antigen‑specific cytotoxicity
Conduct molecular characterization using techniques like ddPCR, qPCR, Sanger sequencing, and next‑generation sequencing to confirm successful gene editing
Work effectively in a cross‑functional team, interacting with process development, analytical development, and regulatory affairs
Document all experimental procedures and results in electronic lab notebooks and technical reports
Present data and findings at internal meetings to advance CAR‑T pipeline programs
Required Experience and Education
Ph.D. in Immunology, Molecular Biology, Cell Biology, Bioengineering, or a related field; or equivalent combination of education and experience
5 years of relevant postdoctoral or industry experience or equivalent experience
Preferred Education and Experience
5 – 8 years relevant post‑doctoral experience
Strong hands‑on experience with human iPSC culture, maintenance, and differentiation protocols
Extensive practical experience with CRISPR/Cas9 or other gene editing technologies in mammalian cells
Proficiency in primary human T‑cell culture and engineering is a strong plus
Expertise in multi‑color flow cytometry and cell sorting for cell characterization
Experience with viral (lentivirus, AAV) and non‑viral gene delivery methods
Excellent communication and interpersonal skills
Ability to work independently and as part of a collaborative, fast‑paced team
Strong problem‑solving and troubleshooting abilities
Demonstrated team management
Demonstrated ability to build and lead a program
Physical Demands
Fine motor movements in fingers/hands to operate computers and other office equipment
Work Environment
Frequently required to sit, stand, walk, stoop, kneel or reach
Possible exposure and handling of laboratory animals
Laboratory atmosphere – possible exposure to chemical, biological or other hazardous substances
Position Type / Expected Hours of Work
This role is currently working onsite and is expected to work onsite for the majority of the working hours. We are a Washington State employer, and the primary work location for Allen Institute employees is 700 Dexter Ave N.; any remote work must be performed in Washington State.
Travel
The successful candidate may be invited to attend occasional national and international conferences
Additional Comments
Relocation assistance
Work visa sponsorship
Annualized Salary Range
$128,050 - $160,050 *
Final salary depends on required education for the role, experience, and level of skills relevant to the role, along with work location, where applicable
Benefits
Employees (and their families) are eligible to enroll in benefits per eligibility rules outlined in the Allen Institute’s Benefits Guide. These benefits include medical, dental, vision, and basic life insurance. Employees are also eligible to enroll in the Allen Institute’s 401k plan. Paid time off is also available as outlined in the Allen Institute’s Benefits Guide.
It is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodation for qualified individuals with disabilities.
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