Bayside Solutions
Research Associate III, Strain Engineering
Base pay range : $45.00/hr - $55.00/hr
Salary Range:
$93,600 - $114,400 per year
Employment type:
Contract
Seniority level:
Mid-Senior level
Job Summary:
The purpose of this position is to engineer and optimize yeast to produce valuable plant natural products for the pharmaceutical industry. The employee will be part of an interdisciplinary, dynamic, and enthusiastic team and must demonstrate a high degree of flexibility, creativity, and initiative. The employee should have a strong desire and ability to learn and contribute as both a leader and team player within a cross‑functional project team.
Duties and Responsibilities:
Formulating scientific hypotheses, constructing new strains, and testing modifications to increase the production of plant natural products in yeast
Applying a range of molecular biology techniques for the design, construction, transformation, and screening of genetically engineered microbial strains and enzyme libraries in bacteria and yeast
Designing and constructing custom DNA sequences (plasmids, linear DNA, gene libraries) for the above via standard molecular biology workflows in E. coli and yeast.
Developing and executing cell‑based assays for strain assessment and functional characterization of various novel proteins according to internal procedures
Facilitating the scheduling and coordination of complex multi‑day experimental schedules and workflows
Analyzing data, documenting, and presenting results and ideas clearly.
Perform other duties as assigned.
Requirements and Qualifications:
B.A./B.S. or M.S. in molecular biology, microbiology, biology, chemistry, engineering, or other related disciplines with five or more years of relevant experience working in a laboratory environment
Proficiency with molecular biology and genetic engineering methods, including PCR, primer design, genomic DNA/plasmid DNA isolation, gel electrophoresis, restriction digest, cloning, DNA sequencing, strain verification by PCR analysis, and experimental troubleshooting strategies for the above
Proficiency with techniques used for DNA construction and assembly, DNA transformation into E. coli and S. cerevisiae
Experience with culturing microbial organisms in microtiter plates and shake flasks
Familiarity with microbial metabolism and/or metabolic pathway databases (e.g., KEGG)
Experience with yeast cultivation and genetic manipulation (transformations, genomic modifications) is required; experience with CRISPR/Cas systems for genetic engineering is a plus.
Ability to work under deadlines and good time management skills
Capability to manage and organize multiple ongoing multi‑day workflows, including scheduling instrumentation, preparing reagents and materials, and interfacing and communicating within and across our interdisciplinary teams
Well‑organized and detail‑oriented, but also flexible enough to work and communicate effectively in a fast‑paced environment
Desired Skills and Experience: Strain engineering, Yeast engineering, Plant natural products, Pharmaceutical industry, Molecular biology, Genetic engineering, PCR, Primer design, Genomic DNA isolation, Plasmid DNA isolation, Gel electrophoresis, Restriction digest, Cloning, DNA sequencing, Strain verification, DNA construction, DNA assembly, E. coli transformation, S. cerevisiae transformation, Microbial culturing, Microtiter plates, Shake flasks, Microbial metabolism, Metabolic pathway databases, KEGG, Yeast cultivation, Yeast genetic manipulation, Genomic modifications, CRISPR/Cas systems, Cell‑based assays, Enzyme libraries, Protein characterization, Laboratory workflows, Experimental design, Data analysis, Cross‑functional collaboration, Project coordination, Time management, Multi‑day experimental scheduling
Bayside Solutions, Inc. is not able to sponsor any candidates at this time. Additionally, candidates for this position must qualify as a W2 candidate.
Bayside Solutions, Inc. may collect your personal information during the position application process. Please reference Bayside Solutions, Inc.'s CCPA Privacy Policy at www.baysidesolutions.com.
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Salary Range:
$93,600 - $114,400 per year
Employment type:
Contract
Seniority level:
Mid-Senior level
Job Summary:
The purpose of this position is to engineer and optimize yeast to produce valuable plant natural products for the pharmaceutical industry. The employee will be part of an interdisciplinary, dynamic, and enthusiastic team and must demonstrate a high degree of flexibility, creativity, and initiative. The employee should have a strong desire and ability to learn and contribute as both a leader and team player within a cross‑functional project team.
Duties and Responsibilities:
Formulating scientific hypotheses, constructing new strains, and testing modifications to increase the production of plant natural products in yeast
Applying a range of molecular biology techniques for the design, construction, transformation, and screening of genetically engineered microbial strains and enzyme libraries in bacteria and yeast
Designing and constructing custom DNA sequences (plasmids, linear DNA, gene libraries) for the above via standard molecular biology workflows in E. coli and yeast.
Developing and executing cell‑based assays for strain assessment and functional characterization of various novel proteins according to internal procedures
Facilitating the scheduling and coordination of complex multi‑day experimental schedules and workflows
Analyzing data, documenting, and presenting results and ideas clearly.
Perform other duties as assigned.
Requirements and Qualifications:
B.A./B.S. or M.S. in molecular biology, microbiology, biology, chemistry, engineering, or other related disciplines with five or more years of relevant experience working in a laboratory environment
Proficiency with molecular biology and genetic engineering methods, including PCR, primer design, genomic DNA/plasmid DNA isolation, gel electrophoresis, restriction digest, cloning, DNA sequencing, strain verification by PCR analysis, and experimental troubleshooting strategies for the above
Proficiency with techniques used for DNA construction and assembly, DNA transformation into E. coli and S. cerevisiae
Experience with culturing microbial organisms in microtiter plates and shake flasks
Familiarity with microbial metabolism and/or metabolic pathway databases (e.g., KEGG)
Experience with yeast cultivation and genetic manipulation (transformations, genomic modifications) is required; experience with CRISPR/Cas systems for genetic engineering is a plus.
Ability to work under deadlines and good time management skills
Capability to manage and organize multiple ongoing multi‑day workflows, including scheduling instrumentation, preparing reagents and materials, and interfacing and communicating within and across our interdisciplinary teams
Well‑organized and detail‑oriented, but also flexible enough to work and communicate effectively in a fast‑paced environment
Desired Skills and Experience: Strain engineering, Yeast engineering, Plant natural products, Pharmaceutical industry, Molecular biology, Genetic engineering, PCR, Primer design, Genomic DNA isolation, Plasmid DNA isolation, Gel electrophoresis, Restriction digest, Cloning, DNA sequencing, Strain verification, DNA construction, DNA assembly, E. coli transformation, S. cerevisiae transformation, Microbial culturing, Microtiter plates, Shake flasks, Microbial metabolism, Metabolic pathway databases, KEGG, Yeast cultivation, Yeast genetic manipulation, Genomic modifications, CRISPR/Cas systems, Cell‑based assays, Enzyme libraries, Protein characterization, Laboratory workflows, Experimental design, Data analysis, Cross‑functional collaboration, Project coordination, Time management, Multi‑day experimental scheduling
Bayside Solutions, Inc. is not able to sponsor any candidates at this time. Additionally, candidates for this position must qualify as a W2 candidate.
Bayside Solutions, Inc. may collect your personal information during the position application process. Please reference Bayside Solutions, Inc.'s CCPA Privacy Policy at www.baysidesolutions.com.
#J-18808-Ljbffr