University of Washington
Research Scientist/Engineer 3 (Temporary)
University of Washington, Seattle, Washington, us, 98127
Temporary Research Scientist/Engineer 3 (UW School of Medicine)
Location: Seattle, WA
As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. The Department of Psychiatry & Behavioral Sciences within the UW School of Medicine currently has an outstanding opportunity for a Temporary Research Scientist/Engineer 3. The Pravetoni lab seeks a scientist to support the development of vaccines & antibody-based therapeutics to counteract substance use disorders and overdoses. This multidisciplinary research program integrates vaccine design, immunomodulators, formulation/delivery platforms, pre‑clinical models, analysis of B and T cell lymphocyte repertoire, innate immunity, GLP/GMP studies and clinical evaluation, and industry collaborations. The position will support activities in vitro & in vivo in a variety of models ranging from cell lines to pre‑clinical studies in small and large animals. The team is currently undertaking Phase I clinical trials of vaccines targeting various opioids and planning additional Phase I trials. (Other threats include SARS‑CoV‑2, cancer, antibiotic bacterial infections.)
Responsibilities Research Operations and Procedures (70%)
Solve complex and varied problems related to ongoing research projects.
Independently perform experimental procedures including hybridoma campaigns, sequencing & cloning of antibody binding regions into suitable vectors for expression, handle cell lines & tissue culture, and analysis of innate immunity in pre‑clinical & clinical samples.
Perform immunological assays such as ELISA, Flow cytometry, aRT-PCR, cytokine multiplex screening.
Execute other experimental tools including sequencing of innate immune cells, B cells, and T cells, and analysis of such data.
Other research aims will involve testing of particles, adjuvants, and other vaccine components.
Immunization and In Vivo Studies (15%)
Exclusively responsible for immunizing mice and rats and collecting tissue.
Assist with in vivo assays to test vaccine and mAb efficacy against target.
Research Support and Documentation (15%)
Support PI and other staff in PowerPoints, posters, peer‑review publications, and patents.
Minimum Requirements
Bachelor’s degree in a related field and four years of relevant experience, OR equivalent combination of education/experience.
Additional Requirements
Relevant experience directly conducting research activities, including analysis of innate and adaptive immunity in samples derived from cell culture, human samples (whole blood), and animal samples (spleen).
Experience using multi‑parameter flow cytometry, ELIspot, ELISA, sequencing workflows, cytokine multiplex assays, qRT-PCR.
Experience in formulation and characterization of adjuvants, particles, delivery systems via biophysical and biological assays.
Desired Qualifications
Knowledge of innate immunity and analysis of dendritic cells & macrophages in vitro and in vivo is strongly preferred.
PhD degree in Pharmacology, Immunology, Biomedical Engineering, or related biomedical fields.
Compensation, Benefits and Position Details Pay Range Minimum
$72,288.00 annual
Pay Range Maximum
$132,612.00 annual
Benefit Information
For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-temporary-per-diem-and-less-than-half-time/
Shift
First Shift (United States of America)
Temporary or Regular?
This is a temporary position
FTE (Full-Time Equivalent)
100.00%
Union/Bargaining Unit
Not Applicable
About The UW Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world. UW employees bring their boundless energy, creative problem‑solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty.
Our Commitment The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81.
To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or dso@uw.edu.
Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law.
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As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. The Department of Psychiatry & Behavioral Sciences within the UW School of Medicine currently has an outstanding opportunity for a Temporary Research Scientist/Engineer 3. The Pravetoni lab seeks a scientist to support the development of vaccines & antibody-based therapeutics to counteract substance use disorders and overdoses. This multidisciplinary research program integrates vaccine design, immunomodulators, formulation/delivery platforms, pre‑clinical models, analysis of B and T cell lymphocyte repertoire, innate immunity, GLP/GMP studies and clinical evaluation, and industry collaborations. The position will support activities in vitro & in vivo in a variety of models ranging from cell lines to pre‑clinical studies in small and large animals. The team is currently undertaking Phase I clinical trials of vaccines targeting various opioids and planning additional Phase I trials. (Other threats include SARS‑CoV‑2, cancer, antibiotic bacterial infections.)
Responsibilities Research Operations and Procedures (70%)
Solve complex and varied problems related to ongoing research projects.
Independently perform experimental procedures including hybridoma campaigns, sequencing & cloning of antibody binding regions into suitable vectors for expression, handle cell lines & tissue culture, and analysis of innate immunity in pre‑clinical & clinical samples.
Perform immunological assays such as ELISA, Flow cytometry, aRT-PCR, cytokine multiplex screening.
Execute other experimental tools including sequencing of innate immune cells, B cells, and T cells, and analysis of such data.
Other research aims will involve testing of particles, adjuvants, and other vaccine components.
Immunization and In Vivo Studies (15%)
Exclusively responsible for immunizing mice and rats and collecting tissue.
Assist with in vivo assays to test vaccine and mAb efficacy against target.
Research Support and Documentation (15%)
Support PI and other staff in PowerPoints, posters, peer‑review publications, and patents.
Minimum Requirements
Bachelor’s degree in a related field and four years of relevant experience, OR equivalent combination of education/experience.
Additional Requirements
Relevant experience directly conducting research activities, including analysis of innate and adaptive immunity in samples derived from cell culture, human samples (whole blood), and animal samples (spleen).
Experience using multi‑parameter flow cytometry, ELIspot, ELISA, sequencing workflows, cytokine multiplex assays, qRT-PCR.
Experience in formulation and characterization of adjuvants, particles, delivery systems via biophysical and biological assays.
Desired Qualifications
Knowledge of innate immunity and analysis of dendritic cells & macrophages in vitro and in vivo is strongly preferred.
PhD degree in Pharmacology, Immunology, Biomedical Engineering, or related biomedical fields.
Compensation, Benefits and Position Details Pay Range Minimum
$72,288.00 annual
Pay Range Maximum
$132,612.00 annual
Benefit Information
For information about benefits for this position, visit https://www.washington.edu/jobs/benefits-for-temporary-per-diem-and-less-than-half-time/
Shift
First Shift (United States of America)
Temporary or Regular?
This is a temporary position
FTE (Full-Time Equivalent)
100.00%
Union/Bargaining Unit
Not Applicable
About The UW Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world. UW employees bring their boundless energy, creative problem‑solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty.
Our Commitment The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81.
To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or dso@uw.edu.
Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law.
#J-18808-Ljbffr