Lead Synthetic Biologist

Develop expertise with multiple gene editing tools allowing knock-in, knock-out, silencing and other genetic manipulation activities Perform wet lab activities related to engineering of mammalian and non-mammalian animal cells Perform flow cytometry Design, produce, and test novel gene editing constructs using molecular cloning and other techniques based on robust, well-controlled scientific experiments (including plasmid digestion, purification, ligation, transformation, and sequencing) Perform In vitro transcription to produce RNA from DNA templates Perform DNA/RNA extraction and downstream analytical assays such as qPCR and RT-qPCR Assist in performance of primary and iPSC-derived cell culture, transfection, and cell-based assays Execute and troubleshoot standard protocols, develop and adapt new protocols, and query literature to incorporate additional assays as needed Independently plan and execute experiments Document all experimental planning, progress, experimental data and genetic designs in electronic lab notebook, present research design and results to multi-disciplinary teams and leadership Create experimental workflows and collect quantitative measurements for engineered cells including flow cytometry and multi-omics techniques Analyze and distill insight from complex molecular data streams Collaborate frequently with computational biologists, protein engineers and cell biologists to incorporate biological design principles and experimental datasets into the synthetic biology product development pipeline Test and characterize molecular and cellular system targets using biochemistry, molecular biology and/or genetic approaches Leverage knowledge of gene editing technologies to contribute scientifically to goals and strategy The ideal candidate will possess the following qualities & qualifications: Ph.D. in Biological Engineering, Synthetic Biology, or a related field + 3 years of experience doing research in an academic or industry lab setting, M.S. + 10 years of experience in related field , or B.S. + 13 years of experience in related field Skilled in molecular biology and cell culture techniques Ability to work both independently and in a collaborative team environment Quantitative data analysis expertise Demonstrated experience in design, analysis and interpretation of in vitro and cell- based assays to assess on and off-target gene editing events using NGS methods (short and long-read) and ddPCR Track record of scientific innovation and thinking outside-of-the-box Ability to communicate complex scientific issues in a logical and coherent manner Experience in flow cytometry, -omics, and analytical techniques a plus Experience with bioproduction and GMP/GLP regulation a plus Experience with gene editing techniques a plus

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