Washington University in St. Louis
Senior Scientist - Genetics
Washington University in St. Louis, Northfield, Vermont, us, 05663
Scheduled Hours
40 Position Summary
The Senior Scientist in the Cremins Lab will work with a dynamic team to conduct research in the generation and analysis of new multimodal imaging data for mapping the neural connectome at synapse resolution using RNA barcodes. The candidate will work as a sub team leader over connectome projects and be responsible for maintaining and organizing lab records, training junior scientists on technical assays, assisting the PI with manuscript preparation and review, and helping with grant content. The role includes designing, optimizing, and standardizing experimental and analysis pipelines to accelerate high-quality publications, conducting in situ sequencing experiments with custom microscopy equipment to image RNA barcodes, standardizing imaging pipelines, collecting data, and mentoring trainees on the microscopes. The candidate will participate in protein and barcode design teams to adjust constructs; ensure imaging infrastructure supports trainees; oversee image acquisition and develop pipelines for image analysis; lead data analysis once acquired to ensure high-quality data; participate in developing new code and statistical methods for in situ sequencing and spatial transcriptomics to map the neural connectome; and integrate data to assess synapse connections and RNA distribution within neurons. Primary Duties & Responsibilities
Collaborates on designing, conducting and reporting research projects and assists with grant preparation. Supervises and conducts Illumina library preparation for genomics experiments, quantification, library pooling, and sequencing runs for the lab. Generates novel in situ sequencing imaging datasets to understand structure-function relationships of neuronal connectomes using confocal microscopy and microfluidics. Processes and analyzes imaging and sequencing data (image registration, segmentation, feature detection, clustering, anchoring to single cell datasets). Oversees and writes established SOPs for molecular sequencing technologies and computational algorithms. Prepares and reviews papers on research. Develops new algorithms for analysis of RNA FISH, DNA FISH, and neural connectome in situ sequencing technologies. Collaborates with computational experts to conduct efficient analyses leading to discoveries in mammalian neuron genome structure-function data. Maintains safety procedures and required lab documentation. Performs other duties as assigned. Working Conditions
Works in a laboratory environment with potential exposure to biological, chemical and radioactive hazards. Must be physically able to wear protective equipment and provide standard care to research animals. The above statements describe the general nature and level of work and are not exhaustive. Management reserves the right to revise or amend duties at any time. Required Qualifications
Education:
PhD or terminal degree; combination of education and experience may substitute for minimum education. Certifications:
No specific certification required. Work Experience:
Postdoctoral (3 years) Skills:
Not Applicable Driver's License:
A driver’s license is not required. More About This Job
Preferred Qualifications: PhD in cell biology, imaging, computational biology or neuroscience. 3+ years of postdoctoral experience is highly preferred. Experience in imaging and technology development is highly preferred. Experience in multi-modal genomics, spatial transcriptomics, computational biology, in vivo AAV or lentivirus injections, behavioral neuroscience, or electrophysiology is a plus. Experience with Illumina library preparation and handling NextSeq/NovaSeq instruments is a plus for mentoring genomics sequencing. Experience in Python/R, Linux, and cluster computing is strongly preferred. Experience in statistical analysis of imaging data is strongly preferred. Ability to work independently with critical thinking and sound judgment. Preferred Qualifications (cont.)
No additional education, certifications, or work experience beyond what is stated elsewhere in the posting. Skills:
Analytical Problem Solving, Cell Cultures, Cluster Computing, Computational Biology, Fluorescence Microscopy, Genomics, Histology, Imaging Techniques, Laboratory Instrumentation, Linux, Python, R, Statistical Analysis, Teamwork, Written Communication Grade:
R12 Salary Range:
$63,400.00 - $115,000.00 / Annually. The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates are determined by qualifications, performance, equity, market rates, and department budget. Questions
For frequently asked questions about the application process, please refer to our External Applicant FAQ. Accommodation
If you are unable to use our online application system or need accommodations, email CandidateQuestions@wustl.edu or call the accommodation number provided. All qualified individuals must be able to perform the essential functions of the position, and reasonable accommodations will be made to enable performance of essential duties, absent undue hardship. Pre-Employment Screening
External candidates offered employment will undergo pre-employment screening as applicable, including criminal background checks and other verification. Offers are contingent upon successful completion of required screening. Benefits Statement
Personal Up to 22 days of vacation, 10 holidays, and sick time. Competitive health insurance with priority appointments and lower copays/coinsurance. Metro transit U-Pass for eligible employees. Retirement plan (403(b)) with university contributions starting at 7%. Wellness Wellness programs, health screenings, mental health resources, EAP, and more. Family 4 weeks caregiver leave; family care resources; tuition for you and family up to 100% undergraduate at WashU after seven years (40% elsewhere). For policies and benefits, visit: https://hr.wustl.edu/benefits/ EEO Statement
WashU is an equal opportunity employer and encourages applications from underrepresented groups. It provides equal opportunity regardless of race, ethnicity, color, national origin, age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. We value an inclusive environment.
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40 Position Summary
The Senior Scientist in the Cremins Lab will work with a dynamic team to conduct research in the generation and analysis of new multimodal imaging data for mapping the neural connectome at synapse resolution using RNA barcodes. The candidate will work as a sub team leader over connectome projects and be responsible for maintaining and organizing lab records, training junior scientists on technical assays, assisting the PI with manuscript preparation and review, and helping with grant content. The role includes designing, optimizing, and standardizing experimental and analysis pipelines to accelerate high-quality publications, conducting in situ sequencing experiments with custom microscopy equipment to image RNA barcodes, standardizing imaging pipelines, collecting data, and mentoring trainees on the microscopes. The candidate will participate in protein and barcode design teams to adjust constructs; ensure imaging infrastructure supports trainees; oversee image acquisition and develop pipelines for image analysis; lead data analysis once acquired to ensure high-quality data; participate in developing new code and statistical methods for in situ sequencing and spatial transcriptomics to map the neural connectome; and integrate data to assess synapse connections and RNA distribution within neurons. Primary Duties & Responsibilities
Collaborates on designing, conducting and reporting research projects and assists with grant preparation. Supervises and conducts Illumina library preparation for genomics experiments, quantification, library pooling, and sequencing runs for the lab. Generates novel in situ sequencing imaging datasets to understand structure-function relationships of neuronal connectomes using confocal microscopy and microfluidics. Processes and analyzes imaging and sequencing data (image registration, segmentation, feature detection, clustering, anchoring to single cell datasets). Oversees and writes established SOPs for molecular sequencing technologies and computational algorithms. Prepares and reviews papers on research. Develops new algorithms for analysis of RNA FISH, DNA FISH, and neural connectome in situ sequencing technologies. Collaborates with computational experts to conduct efficient analyses leading to discoveries in mammalian neuron genome structure-function data. Maintains safety procedures and required lab documentation. Performs other duties as assigned. Working Conditions
Works in a laboratory environment with potential exposure to biological, chemical and radioactive hazards. Must be physically able to wear protective equipment and provide standard care to research animals. The above statements describe the general nature and level of work and are not exhaustive. Management reserves the right to revise or amend duties at any time. Required Qualifications
Education:
PhD or terminal degree; combination of education and experience may substitute for minimum education. Certifications:
No specific certification required. Work Experience:
Postdoctoral (3 years) Skills:
Not Applicable Driver's License:
A driver’s license is not required. More About This Job
Preferred Qualifications: PhD in cell biology, imaging, computational biology or neuroscience. 3+ years of postdoctoral experience is highly preferred. Experience in imaging and technology development is highly preferred. Experience in multi-modal genomics, spatial transcriptomics, computational biology, in vivo AAV or lentivirus injections, behavioral neuroscience, or electrophysiology is a plus. Experience with Illumina library preparation and handling NextSeq/NovaSeq instruments is a plus for mentoring genomics sequencing. Experience in Python/R, Linux, and cluster computing is strongly preferred. Experience in statistical analysis of imaging data is strongly preferred. Ability to work independently with critical thinking and sound judgment. Preferred Qualifications (cont.)
No additional education, certifications, or work experience beyond what is stated elsewhere in the posting. Skills:
Analytical Problem Solving, Cell Cultures, Cluster Computing, Computational Biology, Fluorescence Microscopy, Genomics, Histology, Imaging Techniques, Laboratory Instrumentation, Linux, Python, R, Statistical Analysis, Teamwork, Written Communication Grade:
R12 Salary Range:
$63,400.00 - $115,000.00 / Annually. The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates are determined by qualifications, performance, equity, market rates, and department budget. Questions
For frequently asked questions about the application process, please refer to our External Applicant FAQ. Accommodation
If you are unable to use our online application system or need accommodations, email CandidateQuestions@wustl.edu or call the accommodation number provided. All qualified individuals must be able to perform the essential functions of the position, and reasonable accommodations will be made to enable performance of essential duties, absent undue hardship. Pre-Employment Screening
External candidates offered employment will undergo pre-employment screening as applicable, including criminal background checks and other verification. Offers are contingent upon successful completion of required screening. Benefits Statement
Personal Up to 22 days of vacation, 10 holidays, and sick time. Competitive health insurance with priority appointments and lower copays/coinsurance. Metro transit U-Pass for eligible employees. Retirement plan (403(b)) with university contributions starting at 7%. Wellness Wellness programs, health screenings, mental health resources, EAP, and more. Family 4 weeks caregiver leave; family care resources; tuition for you and family up to 100% undergraduate at WashU after seven years (40% elsewhere). For policies and benefits, visit: https://hr.wustl.edu/benefits/ EEO Statement
WashU is an equal opportunity employer and encourages applications from underrepresented groups. It provides equal opportunity regardless of race, ethnicity, color, national origin, age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. We value an inclusive environment.
#J-18808-Ljbffr