Case Western Reserve University
Research Assistant 2
– Case Western Reserve University Starting wage rate is $19.41 per hour, depending on qualifications, experience, department budgets, and industry data. Employees receive a comprehensive benefits package that includes excellent healthcare, retirement plans, tuition assistance, paid time off, and a winter recess. Position Objective
Working under general supervision, the Research Assistant 2 will plan and carry out research projects in accordance with general research guidelines. Responsibilities include planning and performing experiments on projects related to cardiac contractile function and molecular biology of cardiac muscle contraction. Essential Functions
Plan and carry out research projects that characterize the roles of myofilament contractile proteins in the regulation of cardiac muscle contraction. (6%) Conduct a series of experiments to supply scientific information as part of a larger project. Plan, carry out, quantify, and analyze data from general molecular biology experiments, including design and implementation of cloning strategies, PCR analysis, mutagenesis, expression levels of cardiac proteins by various gel electrophoresis techniques and western blotting, gel purification, plasmid preparation, bacterial transformation, bacterial culture in order to express highly purified recombinant proteins for biophysical experiments. (35%) Plan, carry out, quantify, collect, and analyze data from mechanical experiments in isolated cardiac and skeletal muscle preparations. (35%) May assist in developing improved techniques, projection methods, or procedures related to immunostaining of whole heart sections, isolated muscle fibers, and myofibrils. (10%) Prepare and present research results in lab meetings. (9%) Nonessential Functions
Maintain equipment, prepare buffer/media/reagents, and dispose biohazardous waste. (2%) Maintain lab records; oversee inventory and standard operating procedures. (2%) Perform other duties as assigned. (1%) Contacts
Department : Weekly contact with investigators to discuss research, education, direction, goals and time management; frequent contact with laboratory employees in collaboration on common research projects. University : Occasional interaction with other faculty in the department and the university to provide updates on common collaborative research projects; utilize core university facilities for experimentation and maintain strong relationships with core managers for research collaborations. External : Occasional communication with research collaborators from other institutions. Students : Occasional contact with students to assist them with their research projects. Supervisory Responsibility
No direct supervisory responsibilities. Trains new staff and directs the work of students. Qualifications
Education : Bachelor's degree in physiology, biochemistry, molecular biology or related scientific field and 1 to 3 years of related experience OR Associate’s degree in an approved biotechnology program and 2 to 4 years of experience. Required Skills
Knowledge of commonly used concepts, practices and procedures in chemistry, physics, biochemistry, and molecular/cellular biology and in vivo cardiovascular function. Experience with computer programming and molecular dynamic simulation is preferred. Previous experience working with animals preferred. Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center's mission; handle animals with care and respect at all times. Able to perform various molecular biology techniques. Prior experience with cell culture and protein expression/purification. Ability to follow instructions and pre-established guidelines to perform the functions of the job with minimal supervision. Strong organization skills and maintain a laboratory notebook, attention to scientific detail. Ability to multi-task, prioritize and give attention to detail to complete tasks and meet deadlines. Ability to effectively operate basic molecular and cellular biology laboratory equipment. Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making. Ability to work effectively, both independently and collaboratively within a team. Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter. Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations. Demonstrated history of successful support, education, and advocacy for all students, aligned with the values, mission, and messaging of the university, while adhering to the staff policy on conflict of commitment and interest. Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues and supervisors face to face. Ability to follow through on assigned projects and provide timely follow-up as needed. Ability to meet consistent attendance. Working Conditions
Working conditions include use of a muscle mechanics setup, bench work with molecular reagents and/or cardiac tissues, animal models and bacterial cells in the laboratory, extensive computer use, and work outside standard working days or hours as required. May have exposure to hazards. May require Chemical Safety Training. Personal protective equipment: lab coat, gloves and safety glasses when appropriate. EEO Statement
Case Western Reserve University is an equal opportunity employer. All applicants are protected under federal and state laws and university policy from discrimination based on race, color, religion, sex, sexual orientation, gender identity or expression, national or ethnic origin, protected veteran status, disability, age and genetic information. Case Western Reserve University complies with the Americans with Disabilities Act regarding reasonable accommodations for applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the CWRU Office of Equity to request an accommodation.
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– Case Western Reserve University Starting wage rate is $19.41 per hour, depending on qualifications, experience, department budgets, and industry data. Employees receive a comprehensive benefits package that includes excellent healthcare, retirement plans, tuition assistance, paid time off, and a winter recess. Position Objective
Working under general supervision, the Research Assistant 2 will plan and carry out research projects in accordance with general research guidelines. Responsibilities include planning and performing experiments on projects related to cardiac contractile function and molecular biology of cardiac muscle contraction. Essential Functions
Plan and carry out research projects that characterize the roles of myofilament contractile proteins in the regulation of cardiac muscle contraction. (6%) Conduct a series of experiments to supply scientific information as part of a larger project. Plan, carry out, quantify, and analyze data from general molecular biology experiments, including design and implementation of cloning strategies, PCR analysis, mutagenesis, expression levels of cardiac proteins by various gel electrophoresis techniques and western blotting, gel purification, plasmid preparation, bacterial transformation, bacterial culture in order to express highly purified recombinant proteins for biophysical experiments. (35%) Plan, carry out, quantify, collect, and analyze data from mechanical experiments in isolated cardiac and skeletal muscle preparations. (35%) May assist in developing improved techniques, projection methods, or procedures related to immunostaining of whole heart sections, isolated muscle fibers, and myofibrils. (10%) Prepare and present research results in lab meetings. (9%) Nonessential Functions
Maintain equipment, prepare buffer/media/reagents, and dispose biohazardous waste. (2%) Maintain lab records; oversee inventory and standard operating procedures. (2%) Perform other duties as assigned. (1%) Contacts
Department : Weekly contact with investigators to discuss research, education, direction, goals and time management; frequent contact with laboratory employees in collaboration on common research projects. University : Occasional interaction with other faculty in the department and the university to provide updates on common collaborative research projects; utilize core university facilities for experimentation and maintain strong relationships with core managers for research collaborations. External : Occasional communication with research collaborators from other institutions. Students : Occasional contact with students to assist them with their research projects. Supervisory Responsibility
No direct supervisory responsibilities. Trains new staff and directs the work of students. Qualifications
Education : Bachelor's degree in physiology, biochemistry, molecular biology or related scientific field and 1 to 3 years of related experience OR Associate’s degree in an approved biotechnology program and 2 to 4 years of experience. Required Skills
Knowledge of commonly used concepts, practices and procedures in chemistry, physics, biochemistry, and molecular/cellular biology and in vivo cardiovascular function. Experience with computer programming and molecular dynamic simulation is preferred. Previous experience working with animals preferred. Must demonstrate compliance with Case Western Reserve University animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines. Must demonstrate compassion for animals within Case Western Reserve University facilities and dedication to the Animal Resource Center's mission; handle animals with care and respect at all times. Able to perform various molecular biology techniques. Prior experience with cell culture and protein expression/purification. Ability to follow instructions and pre-established guidelines to perform the functions of the job with minimal supervision. Strong organization skills and maintain a laboratory notebook, attention to scientific detail. Ability to multi-task, prioritize and give attention to detail to complete tasks and meet deadlines. Ability to effectively operate basic molecular and cellular biology laboratory equipment. Ability to problem-solve effectively and assist in the development and implementation of alternative solutions as needed. Must demonstrate good judgment and logical decision-making. Ability to work effectively, both independently and collaboratively within a team. Ability to learn and apply new techniques, ideas, processes, policies, functions, etc. Must be highly motivated and a self-starter. Ability to conform to shifting priorities, demands and timelines. Must be flexible in order to respond to project adjustments and alterations promptly and efficiently. Must be able to adapt to varying workloads and respond appropriately to stressful situations. Demonstrated history of successful support, education, and advocacy for all students, aligned with the values, mission, and messaging of the university, while adhering to the staff policy on conflict of commitment and interest. Excellent verbal and written communication skills; good interpersonal skills with the ability to interact with colleagues and supervisors face to face. Ability to follow through on assigned projects and provide timely follow-up as needed. Ability to meet consistent attendance. Working Conditions
Working conditions include use of a muscle mechanics setup, bench work with molecular reagents and/or cardiac tissues, animal models and bacterial cells in the laboratory, extensive computer use, and work outside standard working days or hours as required. May have exposure to hazards. May require Chemical Safety Training. Personal protective equipment: lab coat, gloves and safety glasses when appropriate. EEO Statement
Case Western Reserve University is an equal opportunity employer. All applicants are protected under federal and state laws and university policy from discrimination based on race, color, religion, sex, sexual orientation, gender identity or expression, national or ethnic origin, protected veteran status, disability, age and genetic information. Case Western Reserve University complies with the Americans with Disabilities Act regarding reasonable accommodations for applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the CWRU Office of Equity to request an accommodation.
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