Ohio State University Wexner Medical Center
Position Summary
The Researcher will be a core technical member of the lab, supporting all aspects of translational research with a focus on CRISPR screening platforms, drug dosing studies in both in vitro and in vivo settings, and clinical sample processing, including development of patient‑derived xenograft (PDX) models.
Responsibilities
Design and execute CRISPR‑Cas9‑based genetic screens in lung cancer cell models.
Culture and manipulate human lung cancer cell lines and organoids, including transfections and viral transductions.
Perform drug sensitivity assays in vitro and conduct dosing studies in mouse xenograft models.
Establish and maintain PDX and CDX models using immunocompromised mouse strains (NSG, NRG, athymic nude).
Process human lung tissue specimens for downstream molecular and cellular analyses, including RNA/protein extraction, flow cytometry and cell sorting, and single‑cell suspensions for sequencing or culture.
Operate lab equipment including GentleMACS Cell Dissociator, flow cytometers, biosafety cabinets, and centrifuges.
Coordinate mouse studies: monitor health, measure tumor burden, maintain accurate animal records in compliance with IACUC protocols.
Prepare experimental reagents, solutions, and maintain lab stocks.
Organize and update biobank/sample databases and maintain meticulous experimental records in ELN or lab notebooks.
Assist in procurement of lab reagents and equipment; coordinate with administrative staff for ordering and service contracts.
Required Qualifications
Master of Science Degree in Life Sciences or a related field.
5 years of relevant lab experience, preferably in cancer biology.
Proficiency in cell culture and standard molecular biology techniques.
Experience handling mice, including tumor implantation and drug administration is a requirement for this position.
Excellent interpersonal skills, problem‑solving flexibility, and adaptability to interface with lab staff.
Adept at planning and prioritizing work to meet commitments with a keen eye for detail.
Action‑oriented and eager to take on new opportunities and tough challenges with a sense of urgency, high energy, and enthusiasm.
Application Requirements
Cover letter (maximum 2 pages) indicating research background, interest in the position, and expected start date.
Curriculum Vitae (CV).
Reprints or PDFs of up to two relevant publications (if available).
Names and contact information of three professional references.
Principal Investigator Dr. Triparna Sen
Professor, Department of Internal Medicine
Associate Director of Research, Division of Medical Oncology
Director, Preclinical Therapeutics Platform
The Ohio State University, The Arthur G. James Comprehensive Cancer Center
508 Biomedical Research Tower
460 W 12th Ave, Columbus, OH 43210
#J-18808-Ljbffr
Responsibilities
Design and execute CRISPR‑Cas9‑based genetic screens in lung cancer cell models.
Culture and manipulate human lung cancer cell lines and organoids, including transfections and viral transductions.
Perform drug sensitivity assays in vitro and conduct dosing studies in mouse xenograft models.
Establish and maintain PDX and CDX models using immunocompromised mouse strains (NSG, NRG, athymic nude).
Process human lung tissue specimens for downstream molecular and cellular analyses, including RNA/protein extraction, flow cytometry and cell sorting, and single‑cell suspensions for sequencing or culture.
Operate lab equipment including GentleMACS Cell Dissociator, flow cytometers, biosafety cabinets, and centrifuges.
Coordinate mouse studies: monitor health, measure tumor burden, maintain accurate animal records in compliance with IACUC protocols.
Prepare experimental reagents, solutions, and maintain lab stocks.
Organize and update biobank/sample databases and maintain meticulous experimental records in ELN or lab notebooks.
Assist in procurement of lab reagents and equipment; coordinate with administrative staff for ordering and service contracts.
Required Qualifications
Master of Science Degree in Life Sciences or a related field.
5 years of relevant lab experience, preferably in cancer biology.
Proficiency in cell culture and standard molecular biology techniques.
Experience handling mice, including tumor implantation and drug administration is a requirement for this position.
Excellent interpersonal skills, problem‑solving flexibility, and adaptability to interface with lab staff.
Adept at planning and prioritizing work to meet commitments with a keen eye for detail.
Action‑oriented and eager to take on new opportunities and tough challenges with a sense of urgency, high energy, and enthusiasm.
Application Requirements
Cover letter (maximum 2 pages) indicating research background, interest in the position, and expected start date.
Curriculum Vitae (CV).
Reprints or PDFs of up to two relevant publications (if available).
Names and contact information of three professional references.
Principal Investigator Dr. Triparna Sen
Professor, Department of Internal Medicine
Associate Director of Research, Division of Medical Oncology
Director, Preclinical Therapeutics Platform
The Ohio State University, The Arthur G. James Comprehensive Cancer Center
508 Biomedical Research Tower
460 W 12th Ave, Columbus, OH 43210
#J-18808-Ljbffr