Exelixis
Senior Scientist I, Protein & Antibody Production
Exelixis, Alameda, California, United States, 94501
Biotherapeutics Group Member
As a member of the Biotherapeutics group, the incumbent will play a key role in supporting the group's discovery and preclinical development efforts by possessing skills and experience in recombinant antibody production and purification/characterization and/or cell line development. The incumbent will be responsible for delivering high quality antibody leads for further development as potential therapeutic candidates or as ADCs. The ideal candidate will have excellent skills and experience in antibody production and characterization including but not limited to upstream protein production (antibody engineering, mammalian cell culture and harvest experience). Downstream protein/antibody experience (protein purification and analytical assessment of antibodies for purity, developability, and stability) and in vitro assessment of antibody binding and function is a plus. Candidates cross-trained on CLD applications (suspension CHO cell culture experience) are encouraged to apply. Essential Duties and Responsibilities: Produce high-quality recombinant antibodies, bispecific antibodies and recombinant proteins to support the group's discovery and pipeline efforts Evaluate, select, manage, and track activities of external CROs, as needed, to support all protein production/characterization needs Be proficient in transient expression technologies (HEK, CHO) to support protein production efforts Onboard and optimize stable cell lines for antigen and antibody production Provide scientific input on protein/antibody production and characterization to support the discovery and preclinical development of biotherapeutic candidates Play a key role in assessing protein structure/function and protein quality as applied towards the evaluation of biologics for developability and large-scale manufacturing Comply with company health and safety policies and general laboratory practices Supervisory Responsibilities: No supervisory responsibilities Education/Experience/Knowledge & Skills: Bachelor's degree in related scientific discipline such as molecular biology, biochemistry, biology or microbiology and a minimum of seven (7) years of experience in the biotechnology/pharmaceutical industry; or, Master's degree in related scientific discipline such as molecular biology, biochemistry, biology or microbiology and a minimum of five (5) years of experience in the biotechnology/pharmaceutical industry. PhD degree in related scientific discipline such as molecular biology, biochemistry, biology or microbiology and zero (0) years of experience in the biotechnology/pharmaceutical industry. Equivalent combination of education and experience Experienced and knowledgeable with demonstrated proficiency and proven track record in mammalian cell culture and mammalian protein expression Extensive hands-on experience in applying mammalian transient expression technologies (HEK, CHO) to support both large scale as well as miniature scale (96w, 24w) medium throughput protein expression Hands-on experience in mammalian cell pool development and single cell clone generation for research as well development/manufacturing applications highly preferred Experience with state-of-the art technologies to support ongoing protein/antibody production and characterization needs, including flow cytometry, ELISA, and high content imaging assays preferred Experience writing summaries and reports, and analyzing, interpreting and presenting scientific data/results to internal and external collaborators Knowledgeable in the purification of proteins and antibodies using various modes of chromatography (affinity, size-exclusion, ion-exchange and HIC) Knowledgeable in protein and antibody characterization suitable to ensure developability and large-scale manufacturing Knowledge/Skills: Solid understanding of mammalian protein/antibody expression (both transient and stable), structure, activity, and biochemical/biophysical characterization Extremely strong attention to technical detail and willingness to cross-train Ability to set goals with an understanding of priorities and impact to programs, the department and the organization Strong communication skills for interacting with external CRO scientists and representatives Ability to design and execute activities/studies aimed at producing and characterizing recombinant proteins/antibodies, to interpret experimental results and to formulate conclusions and next steps based on the data generated Familiarity with general components of biologics formulation and CMC is a plus Experience with automation is highly desirable (Tecan, Hamilton, etc) Prior experience with electronic laboratory notebook documentation and workflow management is a plus Working Conditions: Environment: primarily working in laboratories or in office Exposures encountered: Potential exposure to hazardous chemicals/materials.
As a member of the Biotherapeutics group, the incumbent will play a key role in supporting the group's discovery and preclinical development efforts by possessing skills and experience in recombinant antibody production and purification/characterization and/or cell line development. The incumbent will be responsible for delivering high quality antibody leads for further development as potential therapeutic candidates or as ADCs. The ideal candidate will have excellent skills and experience in antibody production and characterization including but not limited to upstream protein production (antibody engineering, mammalian cell culture and harvest experience). Downstream protein/antibody experience (protein purification and analytical assessment of antibodies for purity, developability, and stability) and in vitro assessment of antibody binding and function is a plus. Candidates cross-trained on CLD applications (suspension CHO cell culture experience) are encouraged to apply. Essential Duties and Responsibilities: Produce high-quality recombinant antibodies, bispecific antibodies and recombinant proteins to support the group's discovery and pipeline efforts Evaluate, select, manage, and track activities of external CROs, as needed, to support all protein production/characterization needs Be proficient in transient expression technologies (HEK, CHO) to support protein production efforts Onboard and optimize stable cell lines for antigen and antibody production Provide scientific input on protein/antibody production and characterization to support the discovery and preclinical development of biotherapeutic candidates Play a key role in assessing protein structure/function and protein quality as applied towards the evaluation of biologics for developability and large-scale manufacturing Comply with company health and safety policies and general laboratory practices Supervisory Responsibilities: No supervisory responsibilities Education/Experience/Knowledge & Skills: Bachelor's degree in related scientific discipline such as molecular biology, biochemistry, biology or microbiology and a minimum of seven (7) years of experience in the biotechnology/pharmaceutical industry; or, Master's degree in related scientific discipline such as molecular biology, biochemistry, biology or microbiology and a minimum of five (5) years of experience in the biotechnology/pharmaceutical industry. PhD degree in related scientific discipline such as molecular biology, biochemistry, biology or microbiology and zero (0) years of experience in the biotechnology/pharmaceutical industry. Equivalent combination of education and experience Experienced and knowledgeable with demonstrated proficiency and proven track record in mammalian cell culture and mammalian protein expression Extensive hands-on experience in applying mammalian transient expression technologies (HEK, CHO) to support both large scale as well as miniature scale (96w, 24w) medium throughput protein expression Hands-on experience in mammalian cell pool development and single cell clone generation for research as well development/manufacturing applications highly preferred Experience with state-of-the art technologies to support ongoing protein/antibody production and characterization needs, including flow cytometry, ELISA, and high content imaging assays preferred Experience writing summaries and reports, and analyzing, interpreting and presenting scientific data/results to internal and external collaborators Knowledgeable in the purification of proteins and antibodies using various modes of chromatography (affinity, size-exclusion, ion-exchange and HIC) Knowledgeable in protein and antibody characterization suitable to ensure developability and large-scale manufacturing Knowledge/Skills: Solid understanding of mammalian protein/antibody expression (both transient and stable), structure, activity, and biochemical/biophysical characterization Extremely strong attention to technical detail and willingness to cross-train Ability to set goals with an understanding of priorities and impact to programs, the department and the organization Strong communication skills for interacting with external CRO scientists and representatives Ability to design and execute activities/studies aimed at producing and characterizing recombinant proteins/antibodies, to interpret experimental results and to formulate conclusions and next steps based on the data generated Familiarity with general components of biologics formulation and CMC is a plus Experience with automation is highly desirable (Tecan, Hamilton, etc) Prior experience with electronic laboratory notebook documentation and workflow management is a plus Working Conditions: Environment: primarily working in laboratories or in office Exposures encountered: Potential exposure to hazardous chemicals/materials.