Pacific States Marine Fisheries Commission
Microbiology Technician 2- Rockfish Genetics Analysis
Pacific States Marine Fisheries Commission, Juneau, Alaska, United States, 99811
Microbiology Technician Position
Group Definition: Microbiology Technicians are concerned with the laboratory techniques used in studying tissues, or microscopic and submicroscopic organisms. They use knowledge of procedural requirements and processes to support research into the causes and prevention of fish diseases. Position/Project Specifics: Rockfish are some of the most commercially important species in Alaska and are also captured as bycatch and in recreational fisheries. Estimating rockfish abundance and distribution patterns of rockfish is challenging because they often live in rocky habitats that are difficult to sample with nets. Additionally, they are difficult and tedious to age, leading to data deficiencies and inconsistencies for a primary data source that is used in stock assessment. Genetic tools have the potential to assist with both of these management challenges. (1) Environmental DNA (eDNA) can potentially be used to characterize rockfish abundance and distribution patterns, especially in untrawlable habitats that cannot be easily surveyed using other methods and (2) epigenetic aging techniques can be used to efficiently estimate rockfish ages. We are seeking a laboratory technician to assist with the continued advancement of these two lines of research. Specifically, the technician will assist with the generation of data from eDNA samples taken in trawlable and untrawlable habitats and assist with the development of a panel of genetic markers that can be used to age Alaskan rockfish with a focus on Pacific ocean perch. We seek someone with the skills to conduct laboratory analyses to produce eDNA data, generate sequencing libraries for epigenetic aging, and design amplicon sequencing panels. The work will entail eDNA extractions, PCR including PCR optimization, designing primers for targeted aging sites, testing those primers on tissue samples and optimizing PCR, and using statistical analyses to conduct quality control on data. Deliverables will include raw sequencing files from eDNA, whole genome methylation, and targeted methylation libraries, and reports summarizing data quality and characteristics from these sequencing files. Essential Functions: Conduct genetics laboratory work including PCR Following established protocols, carry genetics projects using molecular techniques such as DNA sequencing and polymerase chain reaction. Design primers for amplicon sequencing for both eDNA and methylation sequencing applications. Perform field collection activities including collecting samples from fish (e.g. blood, spleen, or kidney), water, and sediment. Identify and recommend minor modifications to laboratory techniques. Knowledge Required by the Position: Laboratory methods in molecular biology/genetics laboratories PCR: primer development, PCR optimization, analyzing results Natural sciences Knowledge of molecular biology. Knowledge of aquatic sciences. Knowledge of Windows-based computer applications such as word processing, spreadsheets, presentation software, database software, molecular biology software. Supervisory Controls: Work is assigned on a daily or weekly basis in terms of project objectives, individual objective, and basic priorities. The employee plans and carries out the assignment with independence, following established protocol. He/she seeks assistance only as needed. When common technical and procedural problems arise, the employee exercises initiative in developing solutions. Accuracy of the data produced, quality of the observations made, and sufficiency of planning and execution are normally accepted without detailed review. Reviews are utilized to ensure that the employee works effectively and produces high quality end products. Guidelines: Guides may be laboratory protocols, analytical protocols, sampling methodologies, standing operating procedures, oral instructions, equipment or instrument manuals, and/or hardware/software manuals. The guides often require careful study or cross-referencing to locate the applicable rule or answer and can involve some interpretation or judgment to resolve the issue. Complexity: The employee is expected to select and execute the proper approach to completing the work. The assigned duties often have steps or processes which vary, depending on factors such as the reason the work is being performed or the conditions under which it is being performed. The employee is expected to exercise independence in recognizing such differences, in choosing the right course of action, and then in selecting and executing the proper approach to completing the work. Scope and Effect: Work products directly affect the design and execution of experiments and the adequacy of long range investigations, tests, or research conclusions. Personal Contacts: Contacts are with employees in PSMFC; federal, state, or tribal agencies; university researchers, private/non-profit organizations, and the general public. Purpose of Contacts: The purpose of contacts is to exchange information and ensure that project goals are being met through coordination and planning. Communication between contacts affords resolution of problematic issues and serves as an information network. Physical Demands: The work is largely sedentary. There is some walking and bending and lifting of materials and supplies. Work Environment: The work is performed in a laboratory environment, with adequate heating, lighting, and ventilation.
Group Definition: Microbiology Technicians are concerned with the laboratory techniques used in studying tissues, or microscopic and submicroscopic organisms. They use knowledge of procedural requirements and processes to support research into the causes and prevention of fish diseases. Position/Project Specifics: Rockfish are some of the most commercially important species in Alaska and are also captured as bycatch and in recreational fisheries. Estimating rockfish abundance and distribution patterns of rockfish is challenging because they often live in rocky habitats that are difficult to sample with nets. Additionally, they are difficult and tedious to age, leading to data deficiencies and inconsistencies for a primary data source that is used in stock assessment. Genetic tools have the potential to assist with both of these management challenges. (1) Environmental DNA (eDNA) can potentially be used to characterize rockfish abundance and distribution patterns, especially in untrawlable habitats that cannot be easily surveyed using other methods and (2) epigenetic aging techniques can be used to efficiently estimate rockfish ages. We are seeking a laboratory technician to assist with the continued advancement of these two lines of research. Specifically, the technician will assist with the generation of data from eDNA samples taken in trawlable and untrawlable habitats and assist with the development of a panel of genetic markers that can be used to age Alaskan rockfish with a focus on Pacific ocean perch. We seek someone with the skills to conduct laboratory analyses to produce eDNA data, generate sequencing libraries for epigenetic aging, and design amplicon sequencing panels. The work will entail eDNA extractions, PCR including PCR optimization, designing primers for targeted aging sites, testing those primers on tissue samples and optimizing PCR, and using statistical analyses to conduct quality control on data. Deliverables will include raw sequencing files from eDNA, whole genome methylation, and targeted methylation libraries, and reports summarizing data quality and characteristics from these sequencing files. Essential Functions: Conduct genetics laboratory work including PCR Following established protocols, carry genetics projects using molecular techniques such as DNA sequencing and polymerase chain reaction. Design primers for amplicon sequencing for both eDNA and methylation sequencing applications. Perform field collection activities including collecting samples from fish (e.g. blood, spleen, or kidney), water, and sediment. Identify and recommend minor modifications to laboratory techniques. Knowledge Required by the Position: Laboratory methods in molecular biology/genetics laboratories PCR: primer development, PCR optimization, analyzing results Natural sciences Knowledge of molecular biology. Knowledge of aquatic sciences. Knowledge of Windows-based computer applications such as word processing, spreadsheets, presentation software, database software, molecular biology software. Supervisory Controls: Work is assigned on a daily or weekly basis in terms of project objectives, individual objective, and basic priorities. The employee plans and carries out the assignment with independence, following established protocol. He/she seeks assistance only as needed. When common technical and procedural problems arise, the employee exercises initiative in developing solutions. Accuracy of the data produced, quality of the observations made, and sufficiency of planning and execution are normally accepted without detailed review. Reviews are utilized to ensure that the employee works effectively and produces high quality end products. Guidelines: Guides may be laboratory protocols, analytical protocols, sampling methodologies, standing operating procedures, oral instructions, equipment or instrument manuals, and/or hardware/software manuals. The guides often require careful study or cross-referencing to locate the applicable rule or answer and can involve some interpretation or judgment to resolve the issue. Complexity: The employee is expected to select and execute the proper approach to completing the work. The assigned duties often have steps or processes which vary, depending on factors such as the reason the work is being performed or the conditions under which it is being performed. The employee is expected to exercise independence in recognizing such differences, in choosing the right course of action, and then in selecting and executing the proper approach to completing the work. Scope and Effect: Work products directly affect the design and execution of experiments and the adequacy of long range investigations, tests, or research conclusions. Personal Contacts: Contacts are with employees in PSMFC; federal, state, or tribal agencies; university researchers, private/non-profit organizations, and the general public. Purpose of Contacts: The purpose of contacts is to exchange information and ensure that project goals are being met through coordination and planning. Communication between contacts affords resolution of problematic issues and serves as an information network. Physical Demands: The work is largely sedentary. There is some walking and bending and lifting of materials and supplies. Work Environment: The work is performed in a laboratory environment, with adequate heating, lighting, and ventilation.