Research Assistant - RNA Therapeutics Core Facility

Research Assistant - RNA Therapeutics Core Facility

We are looking for a talented research assistant to participate in the development of the RNA Therapeutics Core Facility at the Sidney-Altman Hub at the Montreal Clinical Research Institute (IRCM).

You will play a central role in establishing the core facility services and in the execution of activities. This position offers a unique opportunity to contribute to cutting-edge research in RNA therapies and to stimulate innovation in RNA biology.

Summary of responsibilities

Project management and execution:

• Perform research design and planning of RNA therapeutic projects with the core facility manager.
• Carry out laboratory work, including:
• Synthesis, purification, and NGS of sc-pDNA plasmids and vectors.
• Design of RNA sequences and vectors.
• In vitro RNA synthesis, purification, translation, and quality control assays using HPLC, Nanodrop, ELISA, Flow Cytometry, etc.
• Analysis of DNA template quality such as Poly A tail, linearization efficiency, and quantification.
• Quality control and analysis of RNA using Nanodrop, ELISA, nanopore sequencing.
• Formulation of lipid nanoparticles and RNA encapsulation, with quality control monitoring.
• Screening in cell culture for dose-response effects.
• Assist with oligonucleotide synthesis (e.g., ASO, siRNA, sgRNA), including purification, lyophilization, and analysis.
• Apply molecular biology techniques and optimize experimental procedures for plasmid and PCR product analysis, bioconjugation, etc.
• Create, collect, and interpret experimental data following GLP guidelines.
• Assist in establishing quality control measures for RNA, oligonucleotides, and nanolipid particles.

Operational and collaboration:

• Contribute to developing the service offering of the RNA therapeutic core facility.
• Support collaborations with partner labs, institutions, and clients.

Required qualifications:

• Master’s degree (MSc) in molecular biology, biochemistry, bioengineering, or related field.
• Two to three years of industry experience.
• Experience in cell culture, cell-based assays, PCR, in vitro RNA synthesis, HPLC, FPLC, Mass Spectrometry, Capillary Electrophoresis, Lipid nanoparticles, DLS, UF/DF, Nanodrop.
• Strong background in mammalian and bacterial cell culture, molecular biology techniques, and bioanalytical methods.

Knowledge and skills:

• Previous exposure to therapeutic RNA applications.
• Knowledge of RNA biology principles.
• Proficiency with MS-Office, Smart Sheets, Project, Excel, etc.
• Strong analytical, problem-solving, and communication skills.
• Excellent organizational and documentation skills.
• Ability to multitask and prioritize.
• Basic knowledge of oligonucleotide synthesis and ligation.
• Understanding of cGMP requirements.
• Experience with LIMS and QMS is a plus.

Working conditions:

• Full-time temporary position (35 hours/week), with potential extension.
• Location: Offices near Mount Royal Park, Saint-Laurent Boulevard, and the Quartier des Spectacles.
• Competitive salary, benefits, pension plan, medical and disability insurance, telemedicine, EAP, and paid vacation.

How to apply:

Send your resume and cover letter to RecruteRH@ircm.qc.ca, mentioning "Research Assistant – RNA therapeutics core facility" in the subject line.

We thank all applicants; only those selected for an interview will be contacted.