NewCo
Senior Research Associate – iPSC and Cell Biology
NewCo, San Francisco, California, United States, 94199
Overview
We’re building a principled and practical approach to engineering complex tissues. As a Senior Research Associate focused on human iPSCs and advanced cell biology, you’ll help develop and execute the experimental foundation of our tissue engineering platform. This role is ideal for a versatile, hands-on experimentalist who loves lab work. Experience with flow cytometry, microscopy and molecular biology is strongly valued. Experience with 3D culture systems is a strong plus. You’ll work closely with our scientists to develop robust iPSC maintenance and differentiation workflows, advance our 2D/3D culture systems, and generate high-quality data across FACS and microscopy experiments. Your work will directly contribute to core milestones in our early-stage R&D pipeline, from iPSC banking and QC to functional characterization and multi-faceted profiling. What You’ll Do
Maintain, passage, bank, and QC human iPSC lines. Execute and optimize iPSC differentiation protocols (e.g., definitive endoderm, hepatic progenitors, hepatocyte-like cells) with quantitative readouts. Culture a variety of cells in 2D and 3D formats (organoids). Plan and perform flow cytometry panels (antibody selection, titration), sample prep and staining, acquisition/compensation, and gating; analyze data in FlowJo/FCS Express and summarize results. Perform core cell-biology assays: immunocytochemistry/IF, plate-based functional assays, ELISAs, viability and cell survival assays, qPCR/RT‑qPCR. Acquire and analyze microscopy data (widefield/fluorescence; high-content where applicable) and contribute to quantitative pipelines (Fiji/ImageJ). Collaborate across cell biology, tissue engineering, and computational teams to troubleshoot and iterate on design‑build‑test cycles that advance project milestones. Draft and refine SOPs; maintain meticulous records in ELNs (experience with Notion is a plus); contribute to lab operations (inventory, ordering, calibration, sample tracking) to ensure reproducibility and data integrity. Qualifications
B.S. in Cell Biology, Stem Cell Biology, Bioengineering, or a related field with 3–6 years of relevant experience; OR M.S. with 2–3 years of relevant experience. Hands-on experience culturing mammalian cells Practical experience planning and running FACS experiments and analyzing data (compensation, gating, statistics, clear result reporting). Strong foundation in mammalian cell culture, sterile technique, and basic molecular/cell‑biology workflows. Experience with the following: organoid culture; qPCR; high‑content microscopy imaging; ELISAs; functional assays. Proven attention to detail, data integrity, and clear documentation; ability to manage multiple experiments and deadlines in a fast‑moving environment. Strong written and verbal communication skills; comfortable presenting plans and results to interdisciplinary teammates. Preferred Experience
Experience with high-content imaging on multi-well plates, as well as quantitative confocal imaging of 3D cultures/organoids. Familiarity with hydrogels (e.g., Matrigel, PEG, alginate) and microfabrication or microfluidics for cell culture. Startup or industry experience in a research‑intensive environment; enthusiasm for building processes and best practices from the ground up. Who You Are
A careful and creative experimentalist who thrives on iteration and discovery. Excited by the opportunity to deepen your expertise in iPSC biology, advanced cell culture, flow cytometry, and microscopy. Curious and intellectually rigorous, with a hands-on approach to problem solving. Eager to be part of a highly interdisciplinary and collaborative team that values openness, humility, and a shared sense of purpose. Why Join Us
We are a small but mighty team of scientists who believe in a principled approach to tissue engineering. We engage in fun and rigorous research. We welcome scientists who seek an intellectual home but are driven to action—we understand the importance of controls, excellent experimental design, and mastery over the literature, but we are not willing to stop tinkering, exploring, and developing our ideas in the quest for perfection. We put our egos aside and credit teams, not individuals, for progress. That said, we value individuality, and we carefully select our team members, with an emphasis on respecting and encouraging all kinds of diversity. We believe that to understand how different cell types work together, we need many different ways of thinking and working. Join us in shaping the future of regenerative medicine and tissue engineering. Together, we’ll tackle ambitious challenges and push the boundaries of what’s possible. Apply now to be part of our journey! Employment details
Seniority level : Mid-Senior level Employment type : Full-time Job function : Research, Analyst, and Information Technology Base pay range:
$70,000.00/yr - $90,000.00/yr Pay Range:
$70,000–$90,000/year. Initial hire will be a contract position paid hourly, with expectations of conversion to full time after 2 months Work Authorization:
Work authorization in the USA required, we do not offer sponsorship. Start Date:
Immediately
#J-18808-Ljbffr
We’re building a principled and practical approach to engineering complex tissues. As a Senior Research Associate focused on human iPSCs and advanced cell biology, you’ll help develop and execute the experimental foundation of our tissue engineering platform. This role is ideal for a versatile, hands-on experimentalist who loves lab work. Experience with flow cytometry, microscopy and molecular biology is strongly valued. Experience with 3D culture systems is a strong plus. You’ll work closely with our scientists to develop robust iPSC maintenance and differentiation workflows, advance our 2D/3D culture systems, and generate high-quality data across FACS and microscopy experiments. Your work will directly contribute to core milestones in our early-stage R&D pipeline, from iPSC banking and QC to functional characterization and multi-faceted profiling. What You’ll Do
Maintain, passage, bank, and QC human iPSC lines. Execute and optimize iPSC differentiation protocols (e.g., definitive endoderm, hepatic progenitors, hepatocyte-like cells) with quantitative readouts. Culture a variety of cells in 2D and 3D formats (organoids). Plan and perform flow cytometry panels (antibody selection, titration), sample prep and staining, acquisition/compensation, and gating; analyze data in FlowJo/FCS Express and summarize results. Perform core cell-biology assays: immunocytochemistry/IF, plate-based functional assays, ELISAs, viability and cell survival assays, qPCR/RT‑qPCR. Acquire and analyze microscopy data (widefield/fluorescence; high-content where applicable) and contribute to quantitative pipelines (Fiji/ImageJ). Collaborate across cell biology, tissue engineering, and computational teams to troubleshoot and iterate on design‑build‑test cycles that advance project milestones. Draft and refine SOPs; maintain meticulous records in ELNs (experience with Notion is a plus); contribute to lab operations (inventory, ordering, calibration, sample tracking) to ensure reproducibility and data integrity. Qualifications
B.S. in Cell Biology, Stem Cell Biology, Bioengineering, or a related field with 3–6 years of relevant experience; OR M.S. with 2–3 years of relevant experience. Hands-on experience culturing mammalian cells Practical experience planning and running FACS experiments and analyzing data (compensation, gating, statistics, clear result reporting). Strong foundation in mammalian cell culture, sterile technique, and basic molecular/cell‑biology workflows. Experience with the following: organoid culture; qPCR; high‑content microscopy imaging; ELISAs; functional assays. Proven attention to detail, data integrity, and clear documentation; ability to manage multiple experiments and deadlines in a fast‑moving environment. Strong written and verbal communication skills; comfortable presenting plans and results to interdisciplinary teammates. Preferred Experience
Experience with high-content imaging on multi-well plates, as well as quantitative confocal imaging of 3D cultures/organoids. Familiarity with hydrogels (e.g., Matrigel, PEG, alginate) and microfabrication or microfluidics for cell culture. Startup or industry experience in a research‑intensive environment; enthusiasm for building processes and best practices from the ground up. Who You Are
A careful and creative experimentalist who thrives on iteration and discovery. Excited by the opportunity to deepen your expertise in iPSC biology, advanced cell culture, flow cytometry, and microscopy. Curious and intellectually rigorous, with a hands-on approach to problem solving. Eager to be part of a highly interdisciplinary and collaborative team that values openness, humility, and a shared sense of purpose. Why Join Us
We are a small but mighty team of scientists who believe in a principled approach to tissue engineering. We engage in fun and rigorous research. We welcome scientists who seek an intellectual home but are driven to action—we understand the importance of controls, excellent experimental design, and mastery over the literature, but we are not willing to stop tinkering, exploring, and developing our ideas in the quest for perfection. We put our egos aside and credit teams, not individuals, for progress. That said, we value individuality, and we carefully select our team members, with an emphasis on respecting and encouraging all kinds of diversity. We believe that to understand how different cell types work together, we need many different ways of thinking and working. Join us in shaping the future of regenerative medicine and tissue engineering. Together, we’ll tackle ambitious challenges and push the boundaries of what’s possible. Apply now to be part of our journey! Employment details
Seniority level : Mid-Senior level Employment type : Full-time Job function : Research, Analyst, and Information Technology Base pay range:
$70,000.00/yr - $90,000.00/yr Pay Range:
$70,000–$90,000/year. Initial hire will be a contract position paid hourly, with expectations of conversion to full time after 2 months Work Authorization:
Work authorization in the USA required, we do not offer sponsorship. Start Date:
Immediately
#J-18808-Ljbffr