University of Miami
Overview
The University of Miami/UHealth Department of Sylvester Comprehensive Cancer Center - Cancer Proteomics Shared Resource (CPSR) has an exciting opportunity for a Full Time Associate Scientist - Proteomics at the UHealth medical campus in Miami, FL. Job Summary
The Associate Scientist will join CPSR to apply state of the art LC-MS based methods and techniques for quantitative proteomics. You will be responsible for maintenance and operation of a modern high resolution Orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. You will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods for developing diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. Job Functions
Be the primary person responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications. Manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks. Prepare protein samples from a wide range of specimens (e.g., cells, tissues, biofluids, and SDS Page gels). Implement both bottom-up and top-down proteomic approaches. Be responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation). Perform relative quantification of proteins by label-free and stable-isotope labeling strategies (e.g., SILAC, TMT). Be responsible for structural proteomics (e.g., cross-linking, pull-down experiments). Conduct single-cell proteomics analysis. Perform intact protein analysis and molecular weight determination. Use proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis. Be responsible for operation, maintenance, and troubleshooting of a Orbitrap Ascend Tribrid Mass Spectrometer and other laboratory equipment as needed. Assess experimental results and troubleshoot methods and instruments to optimize research protocols. Collaborate with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues. Note: This list of duties is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary. Core Qualifications
Education: Doctorate degree - Philosophy (PhD) required. Ph.D. in Science or Engineering (Biochemistry, Bioengineering, Biology, Chemistry, or related field) preferred. Experience: Minimum 5 years of relevant experience required. Knowledge, Skills and Abilities: Learning Agility: Ability to learn new procedures, technologies, and protocols, and adapt to changing priorities and work demands. Teamwork: Ability to work collaboratively with others and contribute to a team environment. Technical Proficiency: Skilled in using office software, technology, and relevant computer applications. Communication: Strong and clear written and verbal communication skills for interacting with colleagues and stakeholders.
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The University of Miami/UHealth Department of Sylvester Comprehensive Cancer Center - Cancer Proteomics Shared Resource (CPSR) has an exciting opportunity for a Full Time Associate Scientist - Proteomics at the UHealth medical campus in Miami, FL. Job Summary
The Associate Scientist will join CPSR to apply state of the art LC-MS based methods and techniques for quantitative proteomics. You will be responsible for maintenance and operation of a modern high resolution Orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. You will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods for developing diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. Job Functions
Be the primary person responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications. Manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks. Prepare protein samples from a wide range of specimens (e.g., cells, tissues, biofluids, and SDS Page gels). Implement both bottom-up and top-down proteomic approaches. Be responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation). Perform relative quantification of proteins by label-free and stable-isotope labeling strategies (e.g., SILAC, TMT). Be responsible for structural proteomics (e.g., cross-linking, pull-down experiments). Conduct single-cell proteomics analysis. Perform intact protein analysis and molecular weight determination. Use proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis. Be responsible for operation, maintenance, and troubleshooting of a Orbitrap Ascend Tribrid Mass Spectrometer and other laboratory equipment as needed. Assess experimental results and troubleshoot methods and instruments to optimize research protocols. Collaborate with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues. Note: This list of duties is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary. Core Qualifications
Education: Doctorate degree - Philosophy (PhD) required. Ph.D. in Science or Engineering (Biochemistry, Bioengineering, Biology, Chemistry, or related field) preferred. Experience: Minimum 5 years of relevant experience required. Knowledge, Skills and Abilities: Learning Agility: Ability to learn new procedures, technologies, and protocols, and adapt to changing priorities and work demands. Teamwork: Ability to work collaboratively with others and contribute to a team environment. Technical Proficiency: Skilled in using office software, technology, and relevant computer applications. Communication: Strong and clear written and verbal communication skills for interacting with colleagues and stakeholders.
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