Broad Institute
Description & Requirements
The Stevens lab is seeking a dynamic and motivated experimental biologist to join our group in the Program in Brain Health at Broad, to collaboratively design, execute, and manage research in neuroimmunology, synapse development and function, and the impacts of genetic variation on neurodevelopmental and neurodegenerative disorders, such as schizophrenia and Alzheimer’s disease.
The Stevens lab applies cutting‑edge methods in human tissues and human‑relevant models to understand how interactions between the brain’s immune cells, glia, and neurons influence the development and dysfunction of synapses and circuits. We center our work on assessment of gene expression and cellular function in human tissue samples, both from the brain and from peripheral immune compartments like the CSF and blood. Through the lens of human biology, we are able to better understand the fundamental biology and etiology of diseases that are uniquely found in human beings. Our lab collaborates closely across the neurobiology, genetics, and therapeutics groups at the Stanley Center for Psychiatric Research, as well as with many labs across Boston, the U.S., and abroad. A successful candidate in this role will contribute to multiple projects supporting the overall goals of the lab and the Program: to improve brain health through groundbreaking basic and translational research.
Candidates should possess a bachelor’s degree in biology or a related field and at least four years of wet‑lab research experience (or MS and 2+ years of experience). Relevant knowledge and skills include preparation and staining of human tissue; immunohistochemistry; RNAscope; fluorescent confocal microscopy and image analysis; flow cytometry; and basic molecular biology methods (e.g., PCR, DNA/RNA/protein purification, cloning, Western blotting).
Attention to detail, careful note‑keeping, and experience developing, modifying, and troubleshooting experimental protocols are critical, as is clear and open communication with colleagues both within and outside the lab.
The Stevens lab and the Broad Institute provide an outstanding environment for career development through formal scientific and leadership training programs, informal mentorship opportunities, and exposure to diverse, cutting‑edge science and scientists. Our ideal candidate is seeking a longer‑term position in which to grow and develop as a scientist and team leader.
Day‑to‑day responsibilities
Work with team members and collaborators to design and execute experiments essential to the group’s collective success
Work with project manager and lab manager to obtain, manage, and properly store human tissue samples
Section tissues, perform immunohistochemical staining, RNAscope, and other tissue analysis techniques
Perform confocal, light sheet, and light microscopy imaging of tissue samples
Quantitatively assess tissue features such as cellular morphology, synapse number, subcellular features, or frequency of expression of a marker
Document experiments and generate detailed reports in the form of figures, graphs, and slide decks
Consult the literature, supervisor, and scientific staff to research, try, troubleshoot, and optimize new methods that may be relevant to our projects
Liaise with Broad platforms and programs (CDoT, GP/BCL, STP) for access to methods and equipment enabling high-throughput assays
Attend and present data at team meetings and be an active contributor to lab discussions and collaborations
Requirements
Bachelor’s of science degree in cell or molecular biology, neuroscience, or a related field, plus four years of relevant lab experience (or Master’s degree and two years’ experience)
Excellent communication, organization, and time management skills
A high level of initiative and motivation
Ability to work both independently and with a team
Ability to think creatively to solve problems, and to know when to ask for help
Curiosity to learn new things and a passion to make a difference in the lives of patients by advancing the science of psychiatric and neurodegenerative diseases
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The Stevens lab applies cutting‑edge methods in human tissues and human‑relevant models to understand how interactions between the brain’s immune cells, glia, and neurons influence the development and dysfunction of synapses and circuits. We center our work on assessment of gene expression and cellular function in human tissue samples, both from the brain and from peripheral immune compartments like the CSF and blood. Through the lens of human biology, we are able to better understand the fundamental biology and etiology of diseases that are uniquely found in human beings. Our lab collaborates closely across the neurobiology, genetics, and therapeutics groups at the Stanley Center for Psychiatric Research, as well as with many labs across Boston, the U.S., and abroad. A successful candidate in this role will contribute to multiple projects supporting the overall goals of the lab and the Program: to improve brain health through groundbreaking basic and translational research.
Candidates should possess a bachelor’s degree in biology or a related field and at least four years of wet‑lab research experience (or MS and 2+ years of experience). Relevant knowledge and skills include preparation and staining of human tissue; immunohistochemistry; RNAscope; fluorescent confocal microscopy and image analysis; flow cytometry; and basic molecular biology methods (e.g., PCR, DNA/RNA/protein purification, cloning, Western blotting).
Attention to detail, careful note‑keeping, and experience developing, modifying, and troubleshooting experimental protocols are critical, as is clear and open communication with colleagues both within and outside the lab.
The Stevens lab and the Broad Institute provide an outstanding environment for career development through formal scientific and leadership training programs, informal mentorship opportunities, and exposure to diverse, cutting‑edge science and scientists. Our ideal candidate is seeking a longer‑term position in which to grow and develop as a scientist and team leader.
Day‑to‑day responsibilities
Work with team members and collaborators to design and execute experiments essential to the group’s collective success
Work with project manager and lab manager to obtain, manage, and properly store human tissue samples
Section tissues, perform immunohistochemical staining, RNAscope, and other tissue analysis techniques
Perform confocal, light sheet, and light microscopy imaging of tissue samples
Quantitatively assess tissue features such as cellular morphology, synapse number, subcellular features, or frequency of expression of a marker
Document experiments and generate detailed reports in the form of figures, graphs, and slide decks
Consult the literature, supervisor, and scientific staff to research, try, troubleshoot, and optimize new methods that may be relevant to our projects
Liaise with Broad platforms and programs (CDoT, GP/BCL, STP) for access to methods and equipment enabling high-throughput assays
Attend and present data at team meetings and be an active contributor to lab discussions and collaborations
Requirements
Bachelor’s of science degree in cell or molecular biology, neuroscience, or a related field, plus four years of relevant lab experience (or Master’s degree and two years’ experience)
Excellent communication, organization, and time management skills
A high level of initiative and motivation
Ability to work both independently and with a team
Ability to think creatively to solve problems, and to know when to ask for help
Curiosity to learn new things and a passion to make a difference in the lives of patients by advancing the science of psychiatric and neurodegenerative diseases
#J-18808-Ljbffr