Medical University of South Carolina
UNIV - Postdoctoral Scholar - Department of Neurosciences
Medical University of South Carolina, Charleston, South Carolina, United States, 29408
UNIV - Postdoctoral Scholar - Department of Neurosciences
Job Description Summary: The Bharioke Lab at the Medical University of South Carolina is seeking a Postdoctoral Scholar to join our lab in the Department of Neuroscience. This position is ideal for individuals interested in applying cutting edge imaging and computational methods to answer important questions in circuits and developmental neuroscience.
35% Optical recordings and manipulations in head-fixed mice: Learn and perform imaging from head-fixed mice using both one- and two-photon microscopy, to obtain recordings of activity of genetically defined neurons and/or manipulate the activity of groups of genetically defined neurons.
25% Computational analysis of imaging data: Use programming languages, such as MATLAB and Python, to design novel methods to analyze data derived from imaging and behavioral experiments.
20% Conducting behavioral monitoring, habituation, and training in mice: Design and train mice to perform cognitive tasks, reliant on one or more sensory inputs. This may include habituating mice to demonstrate changes in cognitive states (including transitions to and from unconsciousness, and transition to and from sleep), as well as monitoring mice during these changes, both with and without associated optogenetic and chemogenetic manipulations.
10% Conducting surgical procedures in mice: Learn and perform cranial surgeries in both embryonic and/or adult mice, for the purpose of optical imaging and/or manipulations of neurons within cortex. Learn and perform viral injections to target genetically defined populations of neurons (using BSL2 level procedures).
10% Wet lab procedures: Postmortem tissue collection and processing for various assays, including (but not limited to) immunohistochemistry, RNAseq, and spatial transcriptomics.
Additional information: The lab focuses on understanding the rules underlying the organization and function of cortical circuits, throughout the lifespan of the mouse. We employ a variety of techniques in vivo, combining experimental approaches (deep tissue calcium imaging, optogenetics, and chemogenetics) with computational analyses. Experience in these techniques (as demonstrated by prior work/publications) is a plus, along with an inquiring mind and enthusiasm for utilizing and potentially improving novel techniques. The ability to utilize computational methods to analyze large data sets will also be viewed as a plus, though the capacity to learn such methods with the assistance of other lab members will be necessary. The ideal candidate will work independently with initiative and strong work ethic, and will be encouraged to develop ideas collaboratively with the PI and other lab members. Strong communication skills are critical for participating in the lab environment and for collaborations within and beyond the department, and for communicating science to a wider audience. Prior experience presenting scientific work is viewed positively. A calm demeanor is a plus for surgeries and behavioral experiments with rodents.
Employer information and requirements: Medical University of South Carolina (MUSC) is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need. MUSC participates in the federal E-Verify program to confirm the identity and employment authorization of all newly hired employees.
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35% Optical recordings and manipulations in head-fixed mice: Learn and perform imaging from head-fixed mice using both one- and two-photon microscopy, to obtain recordings of activity of genetically defined neurons and/or manipulate the activity of groups of genetically defined neurons.
25% Computational analysis of imaging data: Use programming languages, such as MATLAB and Python, to design novel methods to analyze data derived from imaging and behavioral experiments.
20% Conducting behavioral monitoring, habituation, and training in mice: Design and train mice to perform cognitive tasks, reliant on one or more sensory inputs. This may include habituating mice to demonstrate changes in cognitive states (including transitions to and from unconsciousness, and transition to and from sleep), as well as monitoring mice during these changes, both with and without associated optogenetic and chemogenetic manipulations.
10% Conducting surgical procedures in mice: Learn and perform cranial surgeries in both embryonic and/or adult mice, for the purpose of optical imaging and/or manipulations of neurons within cortex. Learn and perform viral injections to target genetically defined populations of neurons (using BSL2 level procedures).
10% Wet lab procedures: Postmortem tissue collection and processing for various assays, including (but not limited to) immunohistochemistry, RNAseq, and spatial transcriptomics.
Additional information: The lab focuses on understanding the rules underlying the organization and function of cortical circuits, throughout the lifespan of the mouse. We employ a variety of techniques in vivo, combining experimental approaches (deep tissue calcium imaging, optogenetics, and chemogenetics) with computational analyses. Experience in these techniques (as demonstrated by prior work/publications) is a plus, along with an inquiring mind and enthusiasm for utilizing and potentially improving novel techniques. The ability to utilize computational methods to analyze large data sets will also be viewed as a plus, though the capacity to learn such methods with the assistance of other lab members will be necessary. The ideal candidate will work independently with initiative and strong work ethic, and will be encouraged to develop ideas collaboratively with the PI and other lab members. Strong communication skills are critical for participating in the lab environment and for collaborations within and beyond the department, and for communicating science to a wider audience. Prior experience presenting scientific work is viewed positively. A calm demeanor is a plus for surgeries and behavioral experiments with rodents.
Employer information and requirements: Medical University of South Carolina (MUSC) is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need. MUSC participates in the federal E-Verify program to confirm the identity and employment authorization of all newly hired employees.
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