St. Jude Children's Research Hospital
Associate Scientist in Blue Sky Protein Kinases
St. Jude Children's Research Hospital, Memphis, Tennessee, us, 37544
Associate Scientist in Blue Sky Protein Kinases
We are looking for an Associate Scientist to participate in our exciting studies of the human protein kinome. At St. Jude Children’s Research Hospital, world‑class facilities, state‑of‑the‑art technologies, and an open, bench‑to‑bedside environment empower scientists to translate science into survival for children. We encourage you to dream big and stop at nothing in the quest to find cures and save children.
Located in Memphis, Tennessee, this position is part of the Blue Sky Initiative “Seeing the Invisible in Protein Kinases,” an internally funded, interdisciplinary project in the Department of Structural Biology. The project uses nuclear magnetic resonance (NMR) spectroscopy, cryo‑EM, X‑ray crystallography, data science, and computational approaches to structurally characterize the conformational landscape of the human kinome. The goal is to use knowledge of kinase conformations to discover new biology, understand the structural and functional consequences of disease‑associated mutations, and identify novel therapeutic opportunities.
As a member of the biochemistry team, you will prepare high‑quality proteins for structural studies of kinases by NMR, crystallography, and cryo‑EM. The work includes studying multiple kinases, optimizing biochemical and biophysical assays, and contributing to the structural characterization of protein kinases. Your research will advance biophysical and structural knowledge of protein kinases and uncover mechanisms of kinase regulation and new kinase‑mediated biology.
Key Responsibilities
Independently develop and implement construct design, protein purification, kinase activity assays, and biophysical characterization of proteins.
Leverage deep knowledge and hands‑on experience with versatile protein purification technologies to apply established chromatographic and analytical methods for producing high‑quality lead proteins for biochemical, biophysical, and structural studies.
Present problems and propose solutions in discussions with group members and offer insights based on experience to help solve technical or methodological challenges.
Work in a collaborative team environment where results are achieved through effective communication, cooperation, and incorporation of multiple viewpoints.
Perform tasks related to the research project independently but within specific guidelines and subject to regular review by supervisor.
Maintain familiarity with current scientific literature relevant to the research experiments or project.
Manage and train staff as appropriate in laboratory activities.
Preferred Skills and Experience
Experience with protein engineering and design of recombinant proteins.
Expertise in molecular biology and molecular cloning techniques including subcloning, site‑directed mutagenesis, PCR, and DNA manipulation.
Experience with multiple protein expression platforms, including E. coli, insect cell culture, and mammalian cell culture expression systems.
Expertise in protein purification by various chromatographic techniques, including affinity, ion exchange, and size exclusion chromatography.
Experience in protein characterization using techniques that include Mass spec, SEC‑MALS, Differential Scanning Fluorimetry, Dynamic Light Scattering, and Native PAGE.
Experience with in vitro kinase activity assays (preferred).
Experience with analysis of protein‑protein or protein‑ligand interactions using methods that include Surface Plasmon Resonance and Isothermal Titration Calorimetry (preferred).
Strong track record of scientific accomplishments demonstrated through publications in highly regarded journals.
Outstanding organizational and multi‑tasking skills with the ability to manage several simultaneous projects.
Ability and willingness to mentor others and perform as a team member with strong communication skills.
Minimum Education or Training
Bachelor’s degree in a relevant scientific area, such as biochemistry or molecular biology.
Minimum Experience
Seven (7) years of relevant post‑degree work experience for candidates with a bachelor’s degree.
Five (5) years of relevant post‑degree work experience for candidates with a master’s degree.
Two (2) years of relevant post‑degree work experience for candidates with a PhD.
Benefits St. Jude Children’s Research Hospital provides high‑quality health benefits, competitive medical and prescription drug plans, dental and vision coverage, financial security, a retirement plan, a wellness program, and comprehensive time‑off and educational benefits.
Compensation The current salary range for this role is $65,520 – $114,400 per year. Compensation is based on skills, experience, training, licensure, and other business needs.
St. Jude is an Equal Opportunity Employer.
St. Jude Children’s Research Hospital does not accept unsolicited assistance from search firms for employment opportunities.
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Located in Memphis, Tennessee, this position is part of the Blue Sky Initiative “Seeing the Invisible in Protein Kinases,” an internally funded, interdisciplinary project in the Department of Structural Biology. The project uses nuclear magnetic resonance (NMR) spectroscopy, cryo‑EM, X‑ray crystallography, data science, and computational approaches to structurally characterize the conformational landscape of the human kinome. The goal is to use knowledge of kinase conformations to discover new biology, understand the structural and functional consequences of disease‑associated mutations, and identify novel therapeutic opportunities.
As a member of the biochemistry team, you will prepare high‑quality proteins for structural studies of kinases by NMR, crystallography, and cryo‑EM. The work includes studying multiple kinases, optimizing biochemical and biophysical assays, and contributing to the structural characterization of protein kinases. Your research will advance biophysical and structural knowledge of protein kinases and uncover mechanisms of kinase regulation and new kinase‑mediated biology.
Key Responsibilities
Independently develop and implement construct design, protein purification, kinase activity assays, and biophysical characterization of proteins.
Leverage deep knowledge and hands‑on experience with versatile protein purification technologies to apply established chromatographic and analytical methods for producing high‑quality lead proteins for biochemical, biophysical, and structural studies.
Present problems and propose solutions in discussions with group members and offer insights based on experience to help solve technical or methodological challenges.
Work in a collaborative team environment where results are achieved through effective communication, cooperation, and incorporation of multiple viewpoints.
Perform tasks related to the research project independently but within specific guidelines and subject to regular review by supervisor.
Maintain familiarity with current scientific literature relevant to the research experiments or project.
Manage and train staff as appropriate in laboratory activities.
Preferred Skills and Experience
Experience with protein engineering and design of recombinant proteins.
Expertise in molecular biology and molecular cloning techniques including subcloning, site‑directed mutagenesis, PCR, and DNA manipulation.
Experience with multiple protein expression platforms, including E. coli, insect cell culture, and mammalian cell culture expression systems.
Expertise in protein purification by various chromatographic techniques, including affinity, ion exchange, and size exclusion chromatography.
Experience in protein characterization using techniques that include Mass spec, SEC‑MALS, Differential Scanning Fluorimetry, Dynamic Light Scattering, and Native PAGE.
Experience with in vitro kinase activity assays (preferred).
Experience with analysis of protein‑protein or protein‑ligand interactions using methods that include Surface Plasmon Resonance and Isothermal Titration Calorimetry (preferred).
Strong track record of scientific accomplishments demonstrated through publications in highly regarded journals.
Outstanding organizational and multi‑tasking skills with the ability to manage several simultaneous projects.
Ability and willingness to mentor others and perform as a team member with strong communication skills.
Minimum Education or Training
Bachelor’s degree in a relevant scientific area, such as biochemistry or molecular biology.
Minimum Experience
Seven (7) years of relevant post‑degree work experience for candidates with a bachelor’s degree.
Five (5) years of relevant post‑degree work experience for candidates with a master’s degree.
Two (2) years of relevant post‑degree work experience for candidates with a PhD.
Benefits St. Jude Children’s Research Hospital provides high‑quality health benefits, competitive medical and prescription drug plans, dental and vision coverage, financial security, a retirement plan, a wellness program, and comprehensive time‑off and educational benefits.
Compensation The current salary range for this role is $65,520 – $114,400 per year. Compensation is based on skills, experience, training, licensure, and other business needs.
St. Jude is an Equal Opportunity Employer.
St. Jude Children’s Research Hospital does not accept unsolicited assistance from search firms for employment opportunities.
#J-18808-Ljbffr