Ohalo Genetics, Inc
Gene Editing Lab Technician (1st Shift) South San Francisco
Ohalo Genetics, Inc, South San Francisco, California, us, 94083
Overview
Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands-on work, and responsible. Prior experience in a sterile work environment, plant breeding or gene editing is a plus, but is not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role will involve additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work, and offers opportunities to contribute to various aspects of our lab\'s growth.
Responsibilities
Protoplast Isolation, Transfection, and Culture:
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts. Transfect protoplasts using PEG-mediated transfection or electroporation methods. Culture transfected protoplasts to regenerate callus. Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging:
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis:
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records. Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation:
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross-Functional Support and Collaboration:
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes.
Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance.
Performs other duties as assigned
Candidate Profile
Ability to accurately follow verbal and written instructions as well as make suggestions to optimize methodologies
Ability to sit for extended periods of time and do repetitive motions
Ability to take a proactive approach to routine tasks
Must be passionate about learning and enthusiastic about plants and hands-on work
Must have a positive attitude and good interpersonal skills
Ability to ask clarifying questions
Ability to work independently and in a team environment with multiple departments
Must be able to lift up to 50 pounds on intermittent basis and possess the ability to sit for extended periods of time
Candidate must prioritize safety
Desired skills/experience
Experience working in a laboratory setting including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction
Compensation Anticipated Compensation: $29.50/hr
About Ohalo Genetics Ohalo accelerates evolution to unlock nature\’s potential. We use technology to transform agriculture providing farmers new plant varieties with unparalleled yield, efficiency, and profitability while shrinking the land, water and energy footprint of our crops.
Important notices No visa sponsorship is available for this position at this time. No recruiters, please.
#J-18808-Ljbffr
Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands-on work, and responsible. Prior experience in a sterile work environment, plant breeding or gene editing is a plus, but is not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role will involve additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work, and offers opportunities to contribute to various aspects of our lab\'s growth.
Responsibilities
Protoplast Isolation, Transfection, and Culture:
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts. Transfect protoplasts using PEG-mediated transfection or electroporation methods. Culture transfected protoplasts to regenerate callus. Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging:
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis:
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records. Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation:
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross-Functional Support and Collaboration:
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes.
Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance.
Performs other duties as assigned
Candidate Profile
Ability to accurately follow verbal and written instructions as well as make suggestions to optimize methodologies
Ability to sit for extended periods of time and do repetitive motions
Ability to take a proactive approach to routine tasks
Must be passionate about learning and enthusiastic about plants and hands-on work
Must have a positive attitude and good interpersonal skills
Ability to ask clarifying questions
Ability to work independently and in a team environment with multiple departments
Must be able to lift up to 50 pounds on intermittent basis and possess the ability to sit for extended periods of time
Candidate must prioritize safety
Desired skills/experience
Experience working in a laboratory setting including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction
Compensation Anticipated Compensation: $29.50/hr
About Ohalo Genetics Ohalo accelerates evolution to unlock nature\’s potential. We use technology to transform agriculture providing farmers new plant varieties with unparalleled yield, efficiency, and profitability while shrinking the land, water and energy footprint of our crops.
Important notices No visa sponsorship is available for this position at this time. No recruiters, please.
#J-18808-Ljbffr