Ohalo
Overview
Tissue Culture Lab Technician (2nd Shift) at Ohalo Genetics. Location: South San Francisco. Type: Full-time. Shift: 2nd Shift (3pm - 11pm). Desired start date: ASAP. Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands-on work, and responsible. Prior experience in a sterile work environment, plant breeding, or gene editing is a plus but not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role includes additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work and offers opportunities to contribute to various aspects of the lab\'s growth. Responsibilities
Protoplast Isolation, Transfection, and Culture
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts. Transfect protoplasts using PEG-mediated transfection or electroporation methods. Culture transfected protoplasts to regenerate callus. Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records. Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross-Functional Support and Collaboration
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes. Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance. Performs other duties as assigned.
Candidate Profile and Qualifications
Ability to accurately follow verbal and written instructions and to suggest optimizations. Ability to sit for extended periods and perform repetitive tasks. Proactive approach to routine tasks and a passion for learning and hands-on work with plants. Positive attitude and good interpersonal skills; ability to work independently and in a team environment with multiple departments. Ability to lift up to 50 pounds on an intermittent basis. Ability to ask clarifying questions and maintain safety as a priority. Desired skills/experience
Experience in a lab setting with sterile technique, micropropagation, media preparation, microscopy, and DNA extraction. Anticipated Compensation:
$33/hr Notes:
If you previously applied for a job at Ohalo Genetics, you are encouraged to restate your interest by submitting your application. No visa sponsorship is available for this position at this time. No recruiters, please.
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Tissue Culture Lab Technician (2nd Shift) at Ohalo Genetics. Location: South San Francisco. Type: Full-time. Shift: 2nd Shift (3pm - 11pm). Desired start date: ASAP. Ohalo Genetics is seeking a motivated Gene Editing Lab Technician who will contribute to the advancement of plant variety development. The successful candidate will be detail-oriented, enthusiastic about hands-on work, and responsible. Prior experience in a sterile work environment, plant breeding, or gene editing is a plus but not required. The Gene Editing Lab Technician will assist in specific duties to ensure the overall success of the gene editing laboratory. This role includes additional responsibilities related to lab operations, plant inventory maintenance, and process development. This role is not limited to only lab work and offers opportunities to contribute to various aspects of the lab\'s growth. Responsibilities
Protoplast Isolation, Transfection, and Culture
Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts. Transfect protoplasts using PEG-mediated transfection or electroporation methods. Culture transfected protoplasts to regenerate callus. Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging
Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis
Maintain accurate and detailed electronic lab notebooks (ELN) and batch records. Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation
Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross-Functional Support and Collaboration
Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance
Follow safety protocols and institutional guidelines for working with plant tissue and enzymes. Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance. Performs other duties as assigned.
Candidate Profile and Qualifications
Ability to accurately follow verbal and written instructions and to suggest optimizations. Ability to sit for extended periods and perform repetitive tasks. Proactive approach to routine tasks and a passion for learning and hands-on work with plants. Positive attitude and good interpersonal skills; ability to work independently and in a team environment with multiple departments. Ability to lift up to 50 pounds on an intermittent basis. Ability to ask clarifying questions and maintain safety as a priority. Desired skills/experience
Experience in a lab setting with sterile technique, micropropagation, media preparation, microscopy, and DNA extraction. Anticipated Compensation:
$33/hr Notes:
If you previously applied for a job at Ohalo Genetics, you are encouraged to restate your interest by submitting your application. No visa sponsorship is available for this position at this time. No recruiters, please.
#J-18808-Ljbffr