New River Community College
Lab Manager - Department of Cellular, Molecular, and Genetic Medicine
New River Community College, Richmond, Virginia, United States, 23214
Benefits : All full-time university staff are eligible for VCU's robust benefits package that includes comprehensive health benefits, paid annual and holiday leave granted up front, generous tuition benefits, retirement planning and savings options, tax-deferred annuity and cash match programs, employee discounts, well-being resources, abundant opportunities for career development and advancement, and more.
Unit:
School Of Medicine MBU
Department:
Cellular and Genetic Medicine
Position Summary The Department of Cellular, Molecular, and Genetic Medicine is in search of a Lab Manager to jointly work for the laboratories of Dr. Christopher Heier and Dr. Alyson Fiorillo at VCU's School of Medicine. These translational groups are focused on understanding the molecular pathogenesis of muscular dystrophies, on the development of therapies for these conditions, and on developing biomarkers for use in clinical trials. This position would focus on laboratory work that promotes these goals through preclinical drug trials and the characterization of novel mouse models. Additionally, this position will include the performance of molecular biology techniques such as qRT-PCR, Western blots or immunoassays, ELISAs, microscopy and/or immunostaining. In addition, cell culture techniques such as cell passaging, luciferase assays, drug treatments, and the transfection of cell lines is desirable.
Core Responsibilities
Carry out murine preclinical trials for various muscular dystrophy model mice
Mouse handling, mouse colony maintenance, genotyping, functional analysis
Keep up-to-date modifications of all IACUC protocols
Keep up-to-date modifications of all Biosafety protocols
Organize and implement tissue specimen collection and freezing
Prepare samples for RNA, DNA or protein extraction and/or histological analysis
Perform RNA extraction, cDNA synthesis and qPCR analysis using Taqman probes and a Quant Studio 7 Pro; understand the concept of utilizing delta delta ct values to determine relative gene expression
Perform protein extraction, protein quantification and subsequent Western or Jess assays (Protein Simple Analysis)
Perform ELISAs on serum samples or lysed muscle samples
Prepare muscle section for immunostaining or histology staining via cryosectioning
Perform histological stains such as H&E, Sirius Red/Fast Green, or trichrome
Perform immunofluorescence on muscle sections, image using a VS200 Slide Scanning microscope
Perform image analysis of muscle using Image J
Project Implementation
Help implement a data management system for the various projects in the lab
Keep meticulous records from multiple experiments
Write Lab SOPs for new methods developed
Keep inventory records of lab samples and reagents
Anticipate lab reagent needs on a weekly basis
Assist in writing/editing manuscripts
Lab Training
Training of graduate and undergraduate students in basic laboratory practices and techniques
Training new lab members in mouse handling
Qualifications Minimum Qualifications
BS in Science
5 or more years of experience in a laboratory
Standard molecular biology and/or histology techniques
Experience with animal working with mice, working with cell lines and processing tissue
Experience working independently in a laboratory setting
Demonstrated ability to work in and foster an environment of respect, professionalism and civility with a population of faculty, staff, and students from all backgrounds and experiences, or a commitment to do so as a staff member at VCU
Preferred Qualifications
Advanced degree (MS or PhD) in relevant scientific field
Experience with mouse phenotyping, genetic crosses, model characterization, and/or preclinical drug trials
Experience with heart function and/or muscle physiology
Experience with protein-based assays
Experience with cell-based assays
Experience in laboratory training or education
Experience with sequencing techniques or analysis (RNAseq, ChIP-seq, etc.)
Experience with a team science approach
Experience with IACUC including guidelines and protocol submissions
This is a restricted position with no set end date; continued employment is dependent upon project need, availability of funding, and performance.
University Job Code:
32301
VCU is committed to hiring veterans! VCU will include a veteran's period(s) of military service in the calculation of their annual leave accrual rate. This may provide veterans with an increased leave accrual!
Salary Range:
up to $70,000
FLSA Exemption Status:
Exempt
Hours per Week:
40
Restricted Position:
No
ORP Eligible:
No
Flexible Work Arrangement:
Fully Onsite
Contact Information Contact Email:
SOMHR@vcuhealth.org
#J-18808-Ljbffr
Unit:
School Of Medicine MBU
Department:
Cellular and Genetic Medicine
Position Summary The Department of Cellular, Molecular, and Genetic Medicine is in search of a Lab Manager to jointly work for the laboratories of Dr. Christopher Heier and Dr. Alyson Fiorillo at VCU's School of Medicine. These translational groups are focused on understanding the molecular pathogenesis of muscular dystrophies, on the development of therapies for these conditions, and on developing biomarkers for use in clinical trials. This position would focus on laboratory work that promotes these goals through preclinical drug trials and the characterization of novel mouse models. Additionally, this position will include the performance of molecular biology techniques such as qRT-PCR, Western blots or immunoassays, ELISAs, microscopy and/or immunostaining. In addition, cell culture techniques such as cell passaging, luciferase assays, drug treatments, and the transfection of cell lines is desirable.
Core Responsibilities
Carry out murine preclinical trials for various muscular dystrophy model mice
Mouse handling, mouse colony maintenance, genotyping, functional analysis
Keep up-to-date modifications of all IACUC protocols
Keep up-to-date modifications of all Biosafety protocols
Organize and implement tissue specimen collection and freezing
Prepare samples for RNA, DNA or protein extraction and/or histological analysis
Perform RNA extraction, cDNA synthesis and qPCR analysis using Taqman probes and a Quant Studio 7 Pro; understand the concept of utilizing delta delta ct values to determine relative gene expression
Perform protein extraction, protein quantification and subsequent Western or Jess assays (Protein Simple Analysis)
Perform ELISAs on serum samples or lysed muscle samples
Prepare muscle section for immunostaining or histology staining via cryosectioning
Perform histological stains such as H&E, Sirius Red/Fast Green, or trichrome
Perform immunofluorescence on muscle sections, image using a VS200 Slide Scanning microscope
Perform image analysis of muscle using Image J
Project Implementation
Help implement a data management system for the various projects in the lab
Keep meticulous records from multiple experiments
Write Lab SOPs for new methods developed
Keep inventory records of lab samples and reagents
Anticipate lab reagent needs on a weekly basis
Assist in writing/editing manuscripts
Lab Training
Training of graduate and undergraduate students in basic laboratory practices and techniques
Training new lab members in mouse handling
Qualifications Minimum Qualifications
BS in Science
5 or more years of experience in a laboratory
Standard molecular biology and/or histology techniques
Experience with animal working with mice, working with cell lines and processing tissue
Experience working independently in a laboratory setting
Demonstrated ability to work in and foster an environment of respect, professionalism and civility with a population of faculty, staff, and students from all backgrounds and experiences, or a commitment to do so as a staff member at VCU
Preferred Qualifications
Advanced degree (MS or PhD) in relevant scientific field
Experience with mouse phenotyping, genetic crosses, model characterization, and/or preclinical drug trials
Experience with heart function and/or muscle physiology
Experience with protein-based assays
Experience with cell-based assays
Experience in laboratory training or education
Experience with sequencing techniques or analysis (RNAseq, ChIP-seq, etc.)
Experience with a team science approach
Experience with IACUC including guidelines and protocol submissions
This is a restricted position with no set end date; continued employment is dependent upon project need, availability of funding, and performance.
University Job Code:
32301
VCU is committed to hiring veterans! VCU will include a veteran's period(s) of military service in the calculation of their annual leave accrual rate. This may provide veterans with an increased leave accrual!
Salary Range:
up to $70,000
FLSA Exemption Status:
Exempt
Hours per Week:
40
Restricted Position:
No
ORP Eligible:
No
Flexible Work Arrangement:
Fully Onsite
Contact Information Contact Email:
SOMHR@vcuhealth.org
#J-18808-Ljbffr