BioSpace
Job Description
Associate Scientist (Full‑time) – Takeda Development Center Americas, Inc., Cambridge, MA.
Spearhead the development and deployment of high‑throughput platforms to accelerate early discovery of engineered γδT cell therapies. Establish scalable workflows using automated liquid handling and high‑content imaging platforms to streamline construct screening across primary donor sources. Implement robotic protocols for cell isolation, transduction, and expansion in 96‑ and 384‑well formats, enabling parallel testing of CAR variants with consistent throughput and reproducibility. Execute high‑throughput screening of antibody discovery campaigns to identify lead candidates for CAR binder design and optimization. Perform array‑based pooled screening to evaluate novel CAR signaling domain constructs for functional activity. Design, execute, and optimize high‑throughput in‑vitro assays for lead hit identification—including cytokine profiling, 2‑D/3‑D cytotoxicity, and phenotypic flow cytometry. Collaborate with automation engineers and data scientists to troubleshoot workflow issues, validate assay performance, and drive seamless integration of experimental outputs into global systems. Develop standardized SOPs, define QC metrics, and contribute to global harmonization efforts to support assay transferability and alignment across biologics and cell therapy pipelines. Mentor junior scientists, manage laboratory resources, and coordinate cross‑functional project timelines within a matrixed team structure. Present key findings to cross‑functional stakeholders to support early go/no‑go decisions and alignment with pipeline strategy and IND‑enabling milestones.
Requirements
Master’s in Biology, Biotechnology, or related field plus 3 years of related experience OR Bachelor’s in Biology, Biotechnology, or related field plus 5 years of related experience.
Design and execute experiments to assess anti‑tumor functionality of allogeneic innate CAR cell therapies (e.g., Cord blood derived CAR‑NK, CAR‑γδT), including cytokine and cytotoxicity assays and repeat antigen stimulation.
Perform cell isolation, activation, transduction, expansion, and cryopreservation of PBMC‑derived CAR‑abT cells for functional characterization.
Exposure to automated or semi‑automated liquid handling for in‑vitro functional assay set‑up.
Conduct cell isolation, activation, transduction, expansion, and cryopreservation of cord‑blood derived NK cells, ensuring high viability and functionality.
Perform γ‑retroviral vector production and retroviral transduction of innate immune cells (e.g., NK or γδT).
Conduct multiparameter flow cytometry (8+ color) to characterize immune phenotypes.
Cell culture, immunofluorescence imaging, and plasmid cloning.
Compensation & Benefits Full time. Salary range: $99,500.00 – $170,600.00 per year. Competitive compensation and benefits package.
Location Cambridge, MA (United States).
Application Qualified applicants can apply at
https://jobs.takeda.com . Please reference job #R0163160.
Equal Opportunity Employer EOE.
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Requirements
Master’s in Biology, Biotechnology, or related field plus 3 years of related experience OR Bachelor’s in Biology, Biotechnology, or related field plus 5 years of related experience.
Design and execute experiments to assess anti‑tumor functionality of allogeneic innate CAR cell therapies (e.g., Cord blood derived CAR‑NK, CAR‑γδT), including cytokine and cytotoxicity assays and repeat antigen stimulation.
Perform cell isolation, activation, transduction, expansion, and cryopreservation of PBMC‑derived CAR‑abT cells for functional characterization.
Exposure to automated or semi‑automated liquid handling for in‑vitro functional assay set‑up.
Conduct cell isolation, activation, transduction, expansion, and cryopreservation of cord‑blood derived NK cells, ensuring high viability and functionality.
Perform γ‑retroviral vector production and retroviral transduction of innate immune cells (e.g., NK or γδT).
Conduct multiparameter flow cytometry (8+ color) to characterize immune phenotypes.
Cell culture, immunofluorescence imaging, and plasmid cloning.
Compensation & Benefits Full time. Salary range: $99,500.00 – $170,600.00 per year. Competitive compensation and benefits package.
Location Cambridge, MA (United States).
Application Qualified applicants can apply at
https://jobs.takeda.com . Please reference job #R0163160.
Equal Opportunity Employer EOE.
#J-18808-Ljbffr