Tufts University
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Research Technician
role at
Tufts University
Overview The Department of Immunology maintains research programs that focus on translational and basic research in areas relevant to human disease. Our goal is to become a world‑class center for the study of inflammation and the targeted development of novel therapeutics for both treatment and prevention of immune‑mediated diseases.
What You'll Do We are seeking a Research Technician to support studies in T‑cell immunology and autoimmunity. The lab focuses on:
Generating and stabilizing regulatory T (Treg) cells from effector CD4⁺ T cells
Dissecting epigenetic and metabolic pathways that govern Treg lineage stability and fitness (e.g., OXPHOS/heme biosynthesis)
Testing antigen‑specific Treg therapies in mouse models of multiple sclerosis (EAE)
The position involves primary T cell isolation and culture, differentiation assays (Treg/Th17), viral transduction and CRISPR/Cas9 perturbations, multi‑color flow cytometry, mouse colony support and EAE studies (induction, clinical scoring, and tissue processing), and standard molecular biology (qPCR, cloning, Western blotting). The technician will keep accurate records, perform basic analyses (FlowJo, GraphPad Prism; optional R/Python), and help maintain lab operations and regulatory compliance. This role offers substantial hands‑on training, authorship opportunities commensurate with contribution, and growth toward increasing independence. A two‑year commitment is preferred.
Conduct bench research experiments following appropriate protocols and procedures to achieve results
Participate in animal experiments
Record results and maintain accurate records of experiments
Perform limited data interpretation and assist with basic statistics and analysis
Maintain laboratory equipment and order/keep inventory of supplies
Prepare stock solutions, reagents and other media
Participate in the weekly lab Journal Club
What We're Looking For Basic Requirements
Knowledge and skills as typically acquired through a Bachelor’s degree in Biology, Immunology, Molecular Biology, Bioengineering, or related field
Up to 1 year of relevant laboratory experience (including coursework labs); strong sterile technique and attention to detail
Comfort working with mice and willingness to perform animal procedures after training
Proficiency with basic molecular/biochemical techniques (pipetting accuracy, PCR, gel electrophoresis) and basic data analysis (Excel/Prism; maintaining an electronic lab notebook)
Strong organizational and communication skills; reliability, initiative, and the ability to follow SOPs precisely
Ability to occasionally accommodate time‑sensitive experiments (e.g., EAE scoring) outside standard hours
Preferred Qualifications
Experience with primary T‑cell isolation and culture; Treg/Th17 polarization assays
Mouse handling and procedures (e.g., injections, EAE induction, clinical scoring, perfusion, tissue harvest)
Flow cytometry (panel design, compensation, analysis in FlowJo) and/or FACS experience
CRISPR/Cas9 editing; lentiviral/retroviral vector cloning and transduction
qPCR, Western blotting, ELISA, RNA isolation; basic cloning
Experience with data analysis tools (GraphPad Prism; optional R/Python) and figure preparation
Prior exposure to IACUC/IBC protocols and BSL‑2 practices
Interest in a two‑year commitment and growth toward semi‑independent project ownership
Pay Range Minimum $20.50, Midpoint $24.40, Maximum $28.30 Salary is based on related experience, expertise, and internal equity; generally, new hires can expect pay between the minimum and midpoint of the range.
Seniority Level Entry level
Employment Type Full‑time
Job Function Research, Analyst, and Information Technology
Waltham, MA
#J-18808-Ljbffr
Research Technician
role at
Tufts University
Overview The Department of Immunology maintains research programs that focus on translational and basic research in areas relevant to human disease. Our goal is to become a world‑class center for the study of inflammation and the targeted development of novel therapeutics for both treatment and prevention of immune‑mediated diseases.
What You'll Do We are seeking a Research Technician to support studies in T‑cell immunology and autoimmunity. The lab focuses on:
Generating and stabilizing regulatory T (Treg) cells from effector CD4⁺ T cells
Dissecting epigenetic and metabolic pathways that govern Treg lineage stability and fitness (e.g., OXPHOS/heme biosynthesis)
Testing antigen‑specific Treg therapies in mouse models of multiple sclerosis (EAE)
The position involves primary T cell isolation and culture, differentiation assays (Treg/Th17), viral transduction and CRISPR/Cas9 perturbations, multi‑color flow cytometry, mouse colony support and EAE studies (induction, clinical scoring, and tissue processing), and standard molecular biology (qPCR, cloning, Western blotting). The technician will keep accurate records, perform basic analyses (FlowJo, GraphPad Prism; optional R/Python), and help maintain lab operations and regulatory compliance. This role offers substantial hands‑on training, authorship opportunities commensurate with contribution, and growth toward increasing independence. A two‑year commitment is preferred.
Conduct bench research experiments following appropriate protocols and procedures to achieve results
Participate in animal experiments
Record results and maintain accurate records of experiments
Perform limited data interpretation and assist with basic statistics and analysis
Maintain laboratory equipment and order/keep inventory of supplies
Prepare stock solutions, reagents and other media
Participate in the weekly lab Journal Club
What We're Looking For Basic Requirements
Knowledge and skills as typically acquired through a Bachelor’s degree in Biology, Immunology, Molecular Biology, Bioengineering, or related field
Up to 1 year of relevant laboratory experience (including coursework labs); strong sterile technique and attention to detail
Comfort working with mice and willingness to perform animal procedures after training
Proficiency with basic molecular/biochemical techniques (pipetting accuracy, PCR, gel electrophoresis) and basic data analysis (Excel/Prism; maintaining an electronic lab notebook)
Strong organizational and communication skills; reliability, initiative, and the ability to follow SOPs precisely
Ability to occasionally accommodate time‑sensitive experiments (e.g., EAE scoring) outside standard hours
Preferred Qualifications
Experience with primary T‑cell isolation and culture; Treg/Th17 polarization assays
Mouse handling and procedures (e.g., injections, EAE induction, clinical scoring, perfusion, tissue harvest)
Flow cytometry (panel design, compensation, analysis in FlowJo) and/or FACS experience
CRISPR/Cas9 editing; lentiviral/retroviral vector cloning and transduction
qPCR, Western blotting, ELISA, RNA isolation; basic cloning
Experience with data analysis tools (GraphPad Prism; optional R/Python) and figure preparation
Prior exposure to IACUC/IBC protocols and BSL‑2 practices
Interest in a two‑year commitment and growth toward semi‑independent project ownership
Pay Range Minimum $20.50, Midpoint $24.40, Maximum $28.30 Salary is based on related experience, expertise, and internal equity; generally, new hires can expect pay between the minimum and midpoint of the range.
Seniority Level Entry level
Employment Type Full‑time
Job Function Research, Analyst, and Information Technology
Waltham, MA
#J-18808-Ljbffr