NC State University
Postdoctoral Research Scholar
NC State University, Raleigh, North Carolina, United States, 27601
Department ID 196001 - Department of Population Health and Pathobiology
Position Information
Overview The research project will be part of a team focused on developing recombinant infectious laryngotracheitis virus (ILTV)–based vaccines using a CRISPR /Cas9 approach, as well as infectious bronchitis virus (IBV) vaccines using a reverse genetics system. The work will also involve evaluating in vivo vaccine protection and efficacy mechanisms, including animal inoculation, sample collection, data analysis, and interpretation of results.
Responsibilities
Molecular virology tools: Design, plan, and execute research experiments focused on CRISPR /Cas9 gene editing of DNA viruses and the use of reverse genetics systems to edit RNA viruses, including knockout of viral genes and subsequent evaluation of virus-host interactions. Apply advanced recombinant techniques to study and edit viral genomes.
Quantification of viral titers, infectivity, and neutralization titers: Design and develop viral titration and infectivity protocols, and develop neutralization assays and other serum-based fluorescent assays.
Vaccine protection studies: Evaluate the vaccine protection and/or efficacy of vectored and attenuated vaccines.
Data analysis, interpretation, and reporting: Perform statistical analyses of experimental data, interpret findings, and contribute to manuscript preparation, presentations, and research reports. Maintain detailed research records, datasets, and methodological documentation in accordance with project, institutional, and regulatory requirements.
Bioinformatics and transcriptomics: Perform bioinformatics analyses, including RNA‑seq data processing and interpretation.
Team collaboration and training: Work closely with team members and contribute to the training and mentoring of graduate students.
Other Work/Responsibilities
Assist Dr. Reddy in establishing, organizing, and maintaining the molecular virology laboratory.
Perks and Benefits As a Pack member, you belong here, and can enjoy exclusive perks designed to enhance your personal and professional well-being. As you consider this opportunity, we encourage you to review our Employee Value Proposition and learn more about what makes NC State the best place to learn and work for everyone.
Attend non-revenue generating sporting events for free.
Disclaimer: Perks and Benefit eligibility is based on Part-Time or Full-Time Employment status. Eligibility and Employer Sponsored Plans can be found within each of the links offered.
Requirements Preferred Years Experience, Skills, Training, Education
Experience working with gene editing molecular virology tools such as the reverse genetics system and the CRISPR /Cas9 system, with strong hands‑on expertise in molecular cloning strategies.
Proficiency in applying fluorescent microscopy methodologies to study virus‑host interactions.
Experience using advanced DNA and RNA sequencing approaches to characterize circulating viral strains and analyze differential gene expression.
Experience working with poultry research facilities, including isolators and/or floor‑pen systems.
Excellent writing and verbal communication skills in English.
Recruitment NC State University is an equal opportunity employer. All qualified applicants will receive equal opportunities for employment without regard to age, color, disability, gender identity, genetic information, national origin, race, religion, sex (including pregnancy), sexual orientation, and veteran status. The University encourages all qualified applicants, including protected veterans and individuals with disabilities, to apply. Individuals with disabilities requiring disability‑related accommodations in the application and interview process are welcome to contact 919‑513‑0574 to speak with a representative of the Office of Equal Opportunity.
Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. Degree(s) must be obtained prior to start date in order to meet qualifications and receive credit.
Application Process Required Documents: Resume, Cover Letter, Contact Information for References.
NC STATE UNIVERSITY RALEIGH, NC 27695 PHONE: (919) 515-2011
#J-18808-Ljbffr
Position Information
Overview The research project will be part of a team focused on developing recombinant infectious laryngotracheitis virus (ILTV)–based vaccines using a CRISPR /Cas9 approach, as well as infectious bronchitis virus (IBV) vaccines using a reverse genetics system. The work will also involve evaluating in vivo vaccine protection and efficacy mechanisms, including animal inoculation, sample collection, data analysis, and interpretation of results.
Responsibilities
Molecular virology tools: Design, plan, and execute research experiments focused on CRISPR /Cas9 gene editing of DNA viruses and the use of reverse genetics systems to edit RNA viruses, including knockout of viral genes and subsequent evaluation of virus-host interactions. Apply advanced recombinant techniques to study and edit viral genomes.
Quantification of viral titers, infectivity, and neutralization titers: Design and develop viral titration and infectivity protocols, and develop neutralization assays and other serum-based fluorescent assays.
Vaccine protection studies: Evaluate the vaccine protection and/or efficacy of vectored and attenuated vaccines.
Data analysis, interpretation, and reporting: Perform statistical analyses of experimental data, interpret findings, and contribute to manuscript preparation, presentations, and research reports. Maintain detailed research records, datasets, and methodological documentation in accordance with project, institutional, and regulatory requirements.
Bioinformatics and transcriptomics: Perform bioinformatics analyses, including RNA‑seq data processing and interpretation.
Team collaboration and training: Work closely with team members and contribute to the training and mentoring of graduate students.
Other Work/Responsibilities
Assist Dr. Reddy in establishing, organizing, and maintaining the molecular virology laboratory.
Perks and Benefits As a Pack member, you belong here, and can enjoy exclusive perks designed to enhance your personal and professional well-being. As you consider this opportunity, we encourage you to review our Employee Value Proposition and learn more about what makes NC State the best place to learn and work for everyone.
Attend non-revenue generating sporting events for free.
Disclaimer: Perks and Benefit eligibility is based on Part-Time or Full-Time Employment status. Eligibility and Employer Sponsored Plans can be found within each of the links offered.
Requirements Preferred Years Experience, Skills, Training, Education
Experience working with gene editing molecular virology tools such as the reverse genetics system and the CRISPR /Cas9 system, with strong hands‑on expertise in molecular cloning strategies.
Proficiency in applying fluorescent microscopy methodologies to study virus‑host interactions.
Experience using advanced DNA and RNA sequencing approaches to characterize circulating viral strains and analyze differential gene expression.
Experience working with poultry research facilities, including isolators and/or floor‑pen systems.
Excellent writing and verbal communication skills in English.
Recruitment NC State University is an equal opportunity employer. All qualified applicants will receive equal opportunities for employment without regard to age, color, disability, gender identity, genetic information, national origin, race, religion, sex (including pregnancy), sexual orientation, and veteran status. The University encourages all qualified applicants, including protected veterans and individuals with disabilities, to apply. Individuals with disabilities requiring disability‑related accommodations in the application and interview process are welcome to contact 919‑513‑0574 to speak with a representative of the Office of Equal Opportunity.
Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. Degree(s) must be obtained prior to start date in order to meet qualifications and receive credit.
Application Process Required Documents: Resume, Cover Letter, Contact Information for References.
NC STATE UNIVERSITY RALEIGH, NC 27695 PHONE: (919) 515-2011
#J-18808-Ljbffr