Nesco Resource
Job Title: Scientist, Cell-Based Assays
Requirements:
BS/MS/PHD in Biology, Pharmacology, Biochemistry, or related field; BS with 5 years, Masters with 3 years and PHD with 1 year of industry or equivalent lab experience.
Proficiency in mammalian cell culture and 384-well assay workflows
Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays)
Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints)
Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment
Nice to Have:
Experience with automation/LIQ handlers, LIMS/ELN, and statistical QC (Z', CV, S/B)
Familiarity with GraphPad Prism, ActivityBase, or equivalent analysis tools
Hands‑on experience with Echo acoustic dispensing.
Note:
Onsite assignment @ Rahway, NJ.
100% Lab based role.
Overview:
We're seeking a hands‑on scientist to execute high‑throughput cell‑based assays in 384‑well format. You will culture and plate cells, dispense compounds using the Echo, run diverse detection readouts, and analyze dose–response data to deliver high‑quality EC50 results for discovery programs.
Key Responsibilities:
Maintain, expand, and plate mammalian cell lines for 384‑well assays
Set up and execute assays with compound dispensing via Labcyte/Beckman Echo
Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR‑FRET, fluorescence)
Generate dose–response curves (4‑parameter logistic fits) and report EC50/IC50 with QC
Troubleshoot assay performance, optimize Z' factors, and document SOPs/results
Collaborate with discovery teams to prioritize studies and timelines
Additional responsibilities may include setting up western blot assays and learning how to run in‑cell westerns
#J-18808-Ljbffr
BS/MS/PHD in Biology, Pharmacology, Biochemistry, or related field; BS with 5 years, Masters with 3 years and PHD with 1 year of industry or equivalent lab experience.
Proficiency in mammalian cell culture and 384-well assay workflows
Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays)
Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints)
Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment
Nice to Have:
Experience with automation/LIQ handlers, LIMS/ELN, and statistical QC (Z', CV, S/B)
Familiarity with GraphPad Prism, ActivityBase, or equivalent analysis tools
Hands‑on experience with Echo acoustic dispensing.
Note:
Onsite assignment @ Rahway, NJ.
100% Lab based role.
Overview:
We're seeking a hands‑on scientist to execute high‑throughput cell‑based assays in 384‑well format. You will culture and plate cells, dispense compounds using the Echo, run diverse detection readouts, and analyze dose–response data to deliver high‑quality EC50 results for discovery programs.
Key Responsibilities:
Maintain, expand, and plate mammalian cell lines for 384‑well assays
Set up and execute assays with compound dispensing via Labcyte/Beckman Echo
Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR‑FRET, fluorescence)
Generate dose–response curves (4‑parameter logistic fits) and report EC50/IC50 with QC
Troubleshoot assay performance, optimize Z' factors, and document SOPs/results
Collaborate with discovery teams to prioritize studies and timelines
Additional responsibilities may include setting up western blot assays and learning how to run in‑cell westerns
#J-18808-Ljbffr