Calyx
??Scientist II, In Vitro Oncology Research?for Cell Line Development
Calyx, Needham, Massachusetts, United States, 02492
Scientist II
We are seeking a motivated and skilled Scientist II to execute in vitro therapeutic binding, mechanistic and phenotypic assays, utilizing a panel of cancer cell line and organoid models, and is technically skilled with cancer cell banking, cancer cell culture maintenance, characterization and analysis, growth curve development, in support of pharmaceutical discovery and development. The qualified candidate will standardize the culture of cancer cell lines, handle patient-derived material, help develop rodent xenograft models of cancer. The ideal candidate will be experienced in data analysis, interpretation and, be able to prepare presentations to communicate with broader project teams. This position will provide an opportunity for growth and exposure to cutting-edge industry sponsor-driven research projects. Key Responsibilities: Scientific Capabilities: Preferred functioning set of classical skills required (cancer cell lines), including but not limited to: in vitro mechanistic assays and phenotypic assays applying flow cytometry, MSD, ELISA, RT-PCR. Significant experience with culturing immortalized cell lines and/or primary cells models including retrieval of cell lines from cryogenic storage. Expansion and banking of a variety of immortalized cell lines using best practice aseptic techniques. Ensuring the highest quality is maintained through knowledge or performance in cell line quality control methods such as mycoplasma testing, STR fingerprinting, and sterility testing. Data base management of cell line information. Data analysis and recording in ELNs. Maintaining an awareness of current developments in the literature and contributing to the continuous improvement process. Data analysis and recording in ELNs. SOP generation and compliance with in vitro best practices. Mentor and train junior members of the team. With some supervision model execution of: Complex multiplexed assays and high content imaging. Support in vivo studies and terminal necropsies and tissue harvests. Ex vivo assays with rodent tissues. Performs radiolabeling and in-vitro binding assays of radio labeled compounds. Work with radioactivity, radioactive and BSL2 samples. Gamma counting. Sample disposition. Proper handling and disposal of associated materials. Study Support & Execution: Understand and execute on key study steps with minimal supervision. Prioritize tasks according to study timelines. Monitor external partner and sub-contractors for study execution. Communicate with sponsors on an as-needed basis. Serve as an excellent team player and work in collaboration with cross discipline teams. Scientific Communication & Process Improvement: Present study data to internal teams and sponsors. Support regulatory and QA/QC inspections; ensure team readiness and documentation accuracy.
We are seeking a motivated and skilled Scientist II to execute in vitro therapeutic binding, mechanistic and phenotypic assays, utilizing a panel of cancer cell line and organoid models, and is technically skilled with cancer cell banking, cancer cell culture maintenance, characterization and analysis, growth curve development, in support of pharmaceutical discovery and development. The qualified candidate will standardize the culture of cancer cell lines, handle patient-derived material, help develop rodent xenograft models of cancer. The ideal candidate will be experienced in data analysis, interpretation and, be able to prepare presentations to communicate with broader project teams. This position will provide an opportunity for growth and exposure to cutting-edge industry sponsor-driven research projects. Key Responsibilities: Scientific Capabilities: Preferred functioning set of classical skills required (cancer cell lines), including but not limited to: in vitro mechanistic assays and phenotypic assays applying flow cytometry, MSD, ELISA, RT-PCR. Significant experience with culturing immortalized cell lines and/or primary cells models including retrieval of cell lines from cryogenic storage. Expansion and banking of a variety of immortalized cell lines using best practice aseptic techniques. Ensuring the highest quality is maintained through knowledge or performance in cell line quality control methods such as mycoplasma testing, STR fingerprinting, and sterility testing. Data base management of cell line information. Data analysis and recording in ELNs. Maintaining an awareness of current developments in the literature and contributing to the continuous improvement process. Data analysis and recording in ELNs. SOP generation and compliance with in vitro best practices. Mentor and train junior members of the team. With some supervision model execution of: Complex multiplexed assays and high content imaging. Support in vivo studies and terminal necropsies and tissue harvests. Ex vivo assays with rodent tissues. Performs radiolabeling and in-vitro binding assays of radio labeled compounds. Work with radioactivity, radioactive and BSL2 samples. Gamma counting. Sample disposition. Proper handling and disposal of associated materials. Study Support & Execution: Understand and execute on key study steps with minimal supervision. Prioritize tasks according to study timelines. Monitor external partner and sub-contractors for study execution. Communicate with sponsors on an as-needed basis. Serve as an excellent team player and work in collaboration with cross discipline teams. Scientific Communication & Process Improvement: Present study data to internal teams and sponsors. Support regulatory and QA/QC inspections; ensure team readiness and documentation accuracy.