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Shirley Ryan AbilityLab

Research Biologics Technologist 2 - Dr. Ishan Roy

Shirley Ryan AbilityLab, Chicago, Illinois, United States, 60290

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* Molecular Biology and Biochemistry:

+ DNA, RNA, Protein isolation, purification & expression

+ Southern, Northern, Western blotting

+ PCR, RT-PCR,

+ Plasmid preparation

+ Cloning, mapping & sequencing of DNA, single nucleotide polymorphisms detection

+ Recombinant virus production & purification

+ ELISA assays

+ HPLC

+ Mitochondria functional analysis (Oroboros)* Cellular Biology:

+ Cell isolation, cell culture and co-culture

+ Organoids culture

+ Functional studies

+ Transfection and transduction

+ Flow cytometry* Histology and Immunohistochemistry:

+ Tissue fixation/processing: embedding, preparation of blocks & sectioning, staining.

+ Immunohistochemistry

+ Microscopy

+ Tissue quantification* Animal:

+ Husbandry

+ Breeding & screening transgenic mice

+ Dissections

+ Blood collection

+ Surgical procedures

+ Database management of colony

+ Physical and cognitive functional assays* Organ and Tissue Physiology:

+ Making physiological solutions

+ Tissue Dissection

+ In vitro physiological assays

+ Electronic Equipment Calibration and Use* Completes other duties as assigned.* Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related field. Will consider appropriate combination of education and experience in lieu of bachelor’s degree.* Excellent oral and written communication skills in English.* Strong interpersonal and organizational skills needed to participate as a creative member of a multidisciplinary research team.* Ability to exchange ideas and to work closely with clinicians, physical and occupational therapists, patients and professional organizations.* Willingness to network within the Institute and to independently strengthen/develop new external collaborations.* Ability to timely plan and to coordinate project completions.* Laboratory-based position with substantial time spent conducting experiments at the bench.* Office setting for data analysis, writing, and administrative tasks.* Need for protective gear, such as lab coats, gloves, and safety glasses, depending on the experimental procedures and hazards involved.Shirley Ryan AbilityLab is the global leader in physical medicine and rehabilitation for adults and children with the most severe, complex conditions. By joining our team, you’ll be part of our life-changing Mission and Vision. You’ll work in a truly inclusive environment where

through words and actions. You’ll contribute to an innovative culture that is second to none, one that embraces curiosity, discovery and compassion. You’ll play a role in something that’s never been done before as we integrate science and clinical care to help patients achieve better, faster outcomes — as we Advance Human Ability, together. #J-18808-Ljbffr